Studies on the Probiotic, Adhesion and Induction Properties of Artisanal Lactic Acid Bacteria: To Customize a Gastrointestinal Niche to Trigger Anti-Obesity Functions DOI Creative Commons
Aslıhan KAMBER, Çisem Bulut Albayrak,

Hayriye Sebnem Harsa

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 24, 2024

Abstract Aims: The primary goals of this work are to explore the potential probiotic LAB's mucin/mucus layer thickening properties and identify anti-obesity candidate strains that improve appropriate habitat for use with Akkermansia group population in future. Methods Results: The HT-29 cell binding, antimicrobial properties, adhesion layer, growth presence mucin, stability during in vitrogastrointestinal (GI) conditions, biofilm formation, thickness increment abilities were all assessed artisanal LAB strains. Sixteen out 40 chosen further analysis based on their ability withstand GI conditions. Thirteen maintained vitality simulated intestinal fluid, whereas majority displayed high viability gastric juice simulation. Furthermore, 35.2-65.4% those 16 bacteria adhered mucin layer. Besides, different lactate levels produced, Streptococcus thermophilusUIN9 exhibited highest development. Also, a 50 mM concentration had been injected originally, Lacticaseibacillus casei NRRL-B 441 maximum amount (321.6 µg/mL). Conclusions: Two isolates olive from Lactiplantibacillus plantarum as prototype candidates; these did not consume sugars. Significance Impact Study: Probiotic attachment colonic mucosa its stimulate cells secrete mucus critical mechanisms may support development Akkermansia.

Language: Английский

From Bacteria to Host: Deciphering the Impact of Sphingolipid Metabolism on Food Allergic Reactions DOI Creative Commons
Elisa Zubeldia‐Varela, Andrea Macías‐Camero, Marina Pérez‐Gordo

et al.

Current Treatment Options in Allergy, Journal Year: 2023, Volume and Issue: 10(4), P. 384 - 400

Published: Dec. 26, 2023

Abstract Purpose of Review Allergic diseases have become a burden in industrialized societies. Among children, food allergy (FA) constitutes major impairment quality life. FA is partly due to lack or loss tolerance antigens at the level intestinal mucosa, where microbiota plays crucial role. Early changes composition gut may influence development immune system and can be related risk allergic diseases, including FA. This review will focus on role sphingolipids bacteria involved their metabolism, antigen sensitization Recent Findings Numerous studies identified different patterns microbial between individuals with without FA, pointing an interaction microbiota, enterocytes, cells. When this lost imbalance occurs, integrity epithelial barrier altered, leading permeability Gram-negative bacteria, especially those Proteobacteria phylum, been associated Investigating interactions system, function, production metabolites signaling molecules contribute understanding pathogenesis Summary Sphingolipids, class bioactive amphipathic lipids found cell membranes, emerged as critical regulators inflammation. In review, we attempt summarize existing knowledge these metabolism mechanisms underlying

Language: Английский

Citations

1
Heat-killed Lactobacillus paracasei JY56 can relieve ovalbumin-induced food allergy via regulating immuno-inflammatory responses and gut microbiota DOI Creative Commons
Miao Zheng, Ming Ma,

Ruxue Fang

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: unknown

Published: June 1, 2024

Food allergy (FA) is an aberrant immune response triggered by the ingestion of a food antigen. Ovalbumin (OVA)-sensitized and challenged BALB/c mice were orally administered heat-killed (HK)-L. paracasei JY56. In this work, HK-L. paracasei JY56 alleviated FA-induced decrease in body weight rectal temperature reduced score. Serum analysis showed that levels specific antibodies (OVA-sIgE OVA-sIgG) allergic mediators (histamine mast cell protease) FA mice. addition, JY56 also could alleviate OVA-induced suppressing Th2 Th17-type responses, which was evidenced regulation splenic lymphocyte subpopulations associated cytokine secretion. Moreover, jejunal histological intestinal barrier function related gene expression measurement performed to verify repair Meanwhile, TLR4/NF-κB inflammatory pathway activation inhibited at protein levels. Finally, modulate gut microbiota structure increase short-chain fatty acids. conclusion, multiple ways, study provides theoretical basis for application inactivated probiotics functional foods FA.

Language: Английский

Citations

0
Studies on the Probiotic, Adhesion and Induction Properties of Artisanal Lactic Acid Bacteria: To Customize a Gastrointestinal Niche to Trigger Anti-Obesity Functions DOI Creative Commons
Aslıhan KAMBER, Çisem Bulut Albayrak,

Hayriye Sebnem Harsa

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 24, 2024

Abstract Aims: The primary goals of this work are to explore the potential probiotic LAB's mucin/mucus layer thickening properties and identify anti-obesity candidate strains that improve appropriate habitat for use with Akkermansia group population in future. Methods Results: The HT-29 cell binding, antimicrobial properties, adhesion layer, growth presence mucin, stability during in vitrogastrointestinal (GI) conditions, biofilm formation, thickness increment abilities were all assessed artisanal LAB strains. Sixteen out 40 chosen further analysis based on their ability withstand GI conditions. Thirteen maintained vitality simulated intestinal fluid, whereas majority displayed high viability gastric juice simulation. Furthermore, 35.2-65.4% those 16 bacteria adhered mucin layer. Besides, different lactate levels produced, Streptococcus thermophilusUIN9 exhibited highest development. Also, a 50 mM concentration had been injected originally, Lacticaseibacillus casei NRRL-B 441 maximum amount (321.6 µg/mL). Conclusions: Two isolates olive from Lactiplantibacillus plantarum as prototype candidates; these did not consume sugars. Significance Impact Study: Probiotic attachment colonic mucosa its stimulate cells secrete mucus critical mechanisms may support development Akkermansia.

Language: Английский

Citations

0