Probiotic Potential of Enterococcus lactis GL3 Strain Isolated from Honeybee (Apis mellifera L.) Larvae: Insights into Its Antimicrobial Activity Against Paenibacillus larvae

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(2), P. 165 - 165

Published: Feb. 13, 2025

This study aimed to address the need for effective probiotics and antibacterial agents combat American foulbrood disease in honeybees, caused by Paenibacillus larvae. In context of declining honeybee populations due pathogens, we isolated eight lactic acid bacteria (LAB) strains from larvae (Apis mellifera L.) evaluated their probiotic potential inhibitory effects against P. Methods included property assessments, such as bile salt resistance, hydrophobicity, auto-aggregation, co-aggregation with larvae, antioxidant capacities, osmotolerance 50% sucrose, antibiotic susceptibility. Results indicated that GL3 strain exhibited superior attributes potent on Whole-genome sequencing revealed be an Enterococcus lactis genetic features tailored larval gut environment. Pangenome analysis highlighted diversity among E. strains, while molecular docking identified aborycin, a lasso peptide produced GL3, promising inhibitor bacterial cell wall synthesis. These findings suggested was candidate agent health management, warranting further investigation into its vivo efficacy applications beekeeping practices.

Language: Английский

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Gallic acid enhances honeybee larvae resistance to Paenibacillus larvae infections: Insights from molecular docking, bacterial community modulation, and whole‐genome sequencing

Insect Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Abstract American foulbrood (AFB) disease, caused by the bacterium Paenibacillus larvae , is a devastating disease affecting honeybee ( Apis mellifera L.) populations worldwide. Commonly treated with antibiotics, which have negative impacts on both honeybees and environment, there an urgent need for alternatives in AFB control. This study aimed to investigate effects of gallic acid (GA) challenged P . spores explore its modulation larval microbiota. Our results demonstrated that presence spores, coadministration 125 µ g/mL GA significantly increased survival rate body weight larvae. Molecular docking analyses revealed competitively binds spore germination proteins YndE GerM, affinities comparable L‐tyrosine stronger than uric acid, respectively, suggesting interference germination. 16S rRNA gene amplicon sequencing treatment augmented bacterial diversity enriched lactic bacteria (LAB) Whole‐genome 2 LAB strains, Apilactobacillus kunkeei GL‐2 Enterococcus faecium GL‐6, isolated from GA‐treated larvae, unveiled their potential produce antimicrobial secondary metabolites bacteriocins, may contribute competitive advantages against Notably, E. GL‐6 strain possessed genes encoding gallate decarboxylase, enabling utilization, putative bacteriocinogenic genetic clusters enterolysin A enterocin L50 a/b. These findings suggest hold as preventive measures through gut microbiota inhibition P.

Language: Английский

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Beyond Buro: Probiotic and Antifungal Traits of Limosilactobacillus fermentum, from Philippine Burong Isda (Fermented Fish), Revealed by Genomics and Assays

The Microbe, Journal Year: 2025, Volume and Issue: unknown, P. 100334 - 100334

Published: April 1, 2025

Language: Английский

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Isolation and characterization of a marine strain: Bacillus inaquosorum, and its feeding effects on Sebastes schlegelii as feed additive

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742556 - 742556

Published: April 1, 2025

Language: Английский

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Evaluation of the safety and probiotic properties of Limosilactobacillus fermentum BGI-AF16, a uric acid-lowering probiotic strain

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: unknown, P. 107382 - 107382

Published: Feb. 1, 2025

Language: Английский

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