Superior Probiotic Potential of Saccharomyces cerevisiae YH14: In vitro and In vivo Evaluation for Animal Nutrition and Health DOI

Kittiya Khongkool,

Malai Taweechotipatr, Sunchai Payungporn

et al.

Published: May 7, 2025

Abstract The gastrointestinal tract of native animals represents a valuable reservoir probiotic candidates. In this study, yeast strains were isolated from the feces healthy pigs raised in southern Thailand and evaluated for potential, safety, extracellular enzyme production vitro. most promising isolate, Saccharomyces cerevisiae YH14, was selected in vivo evaluation murine model. YH14 exhibited robust growth at 37°C demonstrated high tolerance to acidic (127.38%) bile salt (139.94%) conditions. strain showed strong auto-aggregation (90.09%), cell surface hydrophobicity (59.21%), notable adhesion Caco-2 cells (43.18%). Co-aggregation with enteric pathogens—including enterohemorrhagic E. coli (55.37%), enteropathogenic (57.91%), Salmonella Typhimurium (56.62%)—was observed intermediate levels. non-hemolytic produced beneficial feed enzymes, including xylanase phytase. In vivo, administration induced no mortality, behavioral changes, or adverse effects on intake body weight gain. Histological examination revealed preserved tissue architecture intestine, liver, spleen, signs inflammation. Moreover, modulated gut microbiota by promoting bacterial populations suppressing potential pathogens, without significantly altering overall microbiome diversity. These findings support application S. as additive improving health digestive efficiency livestock.

Language: Английский

Superior Probiotic Potential of Saccharomyces cerevisiae YH14: In vitro and In vivo Evaluation for Animal Nutrition and Health DOI

Kittiya Khongkool,

Malai Taweechotipatr, Sunchai Payungporn

et al.

Published: May 7, 2025

Abstract The gastrointestinal tract of native animals represents a valuable reservoir probiotic candidates. In this study, yeast strains were isolated from the feces healthy pigs raised in southern Thailand and evaluated for potential, safety, extracellular enzyme production vitro. most promising isolate, Saccharomyces cerevisiae YH14, was selected in vivo evaluation murine model. YH14 exhibited robust growth at 37°C demonstrated high tolerance to acidic (127.38%) bile salt (139.94%) conditions. strain showed strong auto-aggregation (90.09%), cell surface hydrophobicity (59.21%), notable adhesion Caco-2 cells (43.18%). Co-aggregation with enteric pathogens—including enterohemorrhagic E. coli (55.37%), enteropathogenic (57.91%), Salmonella Typhimurium (56.62%)—was observed intermediate levels. non-hemolytic produced beneficial feed enzymes, including xylanase phytase. In vivo, administration induced no mortality, behavioral changes, or adverse effects on intake body weight gain. Histological examination revealed preserved tissue architecture intestine, liver, spleen, signs inflammation. Moreover, modulated gut microbiota by promoting bacterial populations suppressing potential pathogens, without significantly altering overall microbiome diversity. These findings support application S. as additive improving health digestive efficiency livestock.

Language: Английский

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