Caenorhabditis elegans as a Screening Model for Probiotics with Properties against Metabolic Syndrome

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 1321 - 1321

Published: Jan. 22, 2024

There is a growing need to develop new approaches prevent and treat diseases related metabolic syndromes, including obesity or type 2 diabetes, that focus on the different factors involved in pathogenesis of these diseases. Due role gut microbiota regulation glucose insulin homeostasis, probiotics with beneficial properties have emerged as an alternative therapeutic tool ameliorate diseases-related disturbances, fat excess inflammation. In last few years, strains bacteria, mainly lactic acid bacteria (LAB) species from genus Bifidobacterium, potential due their anti-obesogenic and/or anti-diabetic properties. However, vivo studies are needed demonstrate mechanisms probiotic features. this context, Caenorhabditis elegans has very powerful simple model study physiological molecular effects applications regarding pathologies syndrome. This review aims summarize main describing anti-obesogenic, anti-diabetic, anti-inflammatory using C. research model, well providing description activities.

Language: Английский

---

Association between gut microbiota and diabetic nephropathy: a mendelian randomization study

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: March 27, 2024

Background The correlation between diabetic nephropathy (DN) and gut microbiota (GM) has been suggested in numerous animal experiments cross-sectional studies. However, a causal association GM DN not ascertained. Methods This research adopted MR analysis to evaluate the link derived from data acquired through publicly available genome-wide studies (GWAS). study utilized inverse variance weighted (IVW) approach assess DN. Four additional methods including MR-Egger, median, mode, simple mode were employed ensure comprehensive robust results. Cochran’s Q test MR-Egger method conducted identify heterogeneity horizontal pleiotropy, respectively. leave-one-out was stability of Finally, reverse performed Results According IVW analysis, Class Verrucomicrobiae ( p = 0.003), Order Verrucomicrobiales Family Verrucomicrobiaceae Genus Akkermansia Catenibacterium 0.031), Coprococcus 1 0.022), Eubacterium hallii group 0.018), Marvinbryantia 0.023) associated with higher risk On contrary, Actinobacteria 0.037), Group ventriosum 0.030), Ruminococcus gauvreauii 0.048), Lactobacillales 0.045), Phylum Proteobacteria 0.017) lower sensitivity did any substantial pleiotropy or outcomes. We found effects on 11 species analysis. Notably, are mutually causalities. Conclusion identified which may enhance understanding intestinal-renal axis provide novel potential targets for early non-invasive diagnosis treatment

Language: Английский

---

Coffee and Tea Consumption and Risk of Type 2 Diabetes in Older Australians

Diabetology, Journal Year: 2025, Volume and Issue: 6(2), P. 12 - 12

Published: Feb. 11, 2025

Background: The prospective relationship between coffee and tea consumption the risk of developing type 2 diabetes mellitus (T2DM) is seldom assessed in older adults. This study investigated association 10-year incidence T2DM Australian Method: Data were collected from participants aged 49 years or above at baseline Blue Mountains Eye Study (n = 1668). Coffee intakes using a validated food frequency questionnaire. was ascertained by self-reported history, fasting blood glucose ≥ 7.0 mmol/L, use medication. Associations discrete-time logistic regression, adjusting for lifestyle demographic factors. Results: Compared to no consumption, intake 1 cup/day associated with lower (multivariate-adjusted HR: 0.46, 95% CI: 0.23, 0.91) follow-up period. However, 2–3 cups/day (HR: 0.66, 0.37, 1.18) ≥4 1.04, 0.52, 2.08) showed significant association. Tea any level not significantly incidence. Results similar after excluding implausible energy intake. Conclusions: In adults, moderate (1 cup/day) incidence, while higher not. lack dose-dependent effect warrants further investigation. These findings should be verified larger studies, considering different types potential age-related genetic

Language: Английский

---

Nanobiotechnology Unveils the Power of Probiotics: A Comprehensive Review on the Synergistic Role of Probiotics and Advanced Nanotechnology in Enhancing Geriatric Health.

Cureus, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

The geriatric population, comprising ages 65 and above, encounters distinct health obstacles because of physiological changes heightened vulnerability to diseases. New technologies are being investigated tackle the intricate requirements this population. Recent advancements in probiotics nanotechnology offer promising strategies enhance by improving nutrient absorption, modulating gut microbiota, delivering targeted therapeutic agents. Probiotics play a crucial role maintaining homeostasis, reducing inflammation, supporting metabolic functions. However, challenges such as limited viability efficacy harsh gastrointestinal conditions hinder their potential. Advanced can overcome these constraints enhancing through nano-encapsulation, controlled delivery, improvement bioavailability. This review explores synergistic potential advanced addressing age-related concerns. It highlights key developments probiotic formulations, nano-based delivery systems, combined impact on health, immunity, neuroprotection. convergence represents novel transformative approach promoting healthy aging, paving way for innovative interventions.

Language: Английский

---

Probiotics, prebiotics, synbiotics and other microbiome-based innovative therapeutics to mitigate obesity and enhance longevity via the gut-brain axis

Microbiome Research Reports, Journal Year: 2024, Volume and Issue: 3(2)

Published: May 17, 2024

The global prevalence of obesity currently exceeds 1 billion people and is accompanied by an increase in the aging population. Obesity share many hallmarks are leading risk factors for cardiometabolic disease premature death. Current anti-obesity pro-longevity pharmacotherapies limited side effects, warranting development novel therapies. gut microbiota plays a major role human health disease, with dysbiotic composition evident obese aged individuals. bidirectional communication system between central nervous system, known as gut-brain axis, may link to unhealthy aging. Modulating microbiome-targeted therapies, such biotics, strategy treat and/or manage promote longevity. Biotics represent material derived from living or once-living organisms, which have therapeutic effects. Pre-, pro-, syn- post-biotics beneficially modulate microbial function improve process. However, investigation biotics next-generation therapeutics has only just begun. Further research needed identify understand their mechanisms action. Investigating axis lead strategies obese, comorbid (e.g., sarcopenic obese) patient populations. This review discusses interrelationship aging, particular emphasis on microbiome, presents agents obesity, related states.

Language: Английский

---

Time‐restricted feeding mediated modulation of microbiota leads to changes in muscle physiology in Drosophila obesity models

Aging Cell, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 24, 2024

Abstract Recent research has highlighted the essential role of microbiome in maintaining skeletal muscle physiology. The microbiota influences health by regulating lipid metabolism, protein synthesis, and insulin sensitivity. However, metabolic disturbances such as obesity can lead to dysbiosis, impairing function. Time‐restricted feeding (TRF) been shown mitigate obesity‐related dysfunction, but its effects on restoring healthy microbiomes remain poorly understood. This study utilizes 16S analysis bacterial supplementation investigate communities influenced TRF that may benefit In wild‐type obese Drosophila models (axenic devoid natural microbial communities), absence influence performance metabolism differently. Specifically, axenic exhibited reduced performance, higher glucose levels, resistance, ectopic accumulation, decreased ATP levels. Interestingly, (induced a high‐fat diet or predisposed mutant Sk2), improved lowered increased was found modulate composition, notably increasing Acetobacter pasteurianus (AP) decreasing Staphylococcus aureus (SA) both models. Supplementation with AP while SA had opposite effect. provides novel insights into complex interactions between TRF, microbiota, physiology different

Language: Английский

---

Circulatory dietary and gut-derived metabolites predict preclinical Alzheimer's disease.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 10, 2024

Abstract A key component of disease prevention is the identification at-risk individuals. Microbial dysbiosis and microbe-derived metabolites (MDM) can influence central nervous system, but their role in progression as prognostic indicators unknown. To identify preclinical factors associated with Alzheimer’s (AD), we compared gut microbiome metabolome profiles cognitively healthy subjects, subjective cognitive impairment (SCI) participants mild (MCI) (n=50 per group, matched for age, BMI sex), targeting previously health (TMAO, bile acids, tryptophan, p -cresol derivatives). 16S rRNA bacterial sequencing targeted LC-MS/MS were employed faecal speciation serum MDM quantification. Microbiome beta diversity differed between controls SCI participants. Multiple linear regression modelling highlighted five (indoxyl sulfate, choline, 5-hydroxyindole acetic acid, indole-3-propionic acid (IPA) kynurenic acid) significantly altered AD. Neuroprotective metabolites, including IPA, exhibited lower concentrations MCI comparison to controls, while cytotoxic metabolite indoxyl sulfate had higher levels. Random Forest algorithm multiclass classification confirmed extended our results, identifying six kynurenine) predictors early decline, an area under curve 0.74. In summary, a combined statistical machine learning approach identified novel composite risk factor future dementia risk.

Language: Английский

---

Distinct Gut Microbiota Signatures in Order People with Sarcopenia without Obesity and Sarcopenic Obesity

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 30, 2024

Background Previous evidence suggests that gut dysbiosis plays an important role in the development and progression of sarcopenia sarcopenic obesity, but supporting this association is lacking. Thus, study aimed to investigate characteristics microbiota older people with obesity. Methods The involved 60 elderly individuals aged 65 years or older, recruited from Chongming, Shanghai, China. Participants were categorized into three groups based on Asian Working Group for Sarcopenia 2019 (AWGS 2019) World Health Organization obesity guidelines: (SO, n = 20), without (Sar, 18), control (Con, 22). was analyzed by sequencing V3-V4 regions 16S rRNA gene. Results Significant differences diversity composition observed Sar SO groups. A reduction alpha diversity, as measured Chao1 ACE index, found group. Beta unweighted Unifrac significantly different between SO, Sar, Con Bacterial community analysis showed at family genus level, relative abundance Rikenellaceae, Christensenellacea, Alistipes, Christensenellaceae R-7 group, Ruminococcus, Odoribacter abundant group, whereas Enterobacteriaceae more enriched Furthermore, revealed linear discriminant effect size (LEfSe) receiver operating curve (ROC) analysis, Alistipes group identified biomarker diagnosis a Conclusion This demonstrated have distinct profiles. results provide new insights highlighting potential therapeutic target condition.

Language: Английский

---

Postcholecystectomy Gut Microbiome Changes and the Clinical Impact: A Systematic Review With Narrative Synthesis

Journal of Gastroenterology and Hepatology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 15, 2024

ABSTRACT Background Cholecystectomy (CCE) can affect the enterohepatic circulation of bile acids and result in gut microbiome changes. This systematic review aimed to clarify effect CCE on composition its clinical impact. Method A search was conducted PubMed, Web Science, Scopus, combining keywords such as “cholecystectomy” or “post‐cholecystectomy” with “gut microbiome,” “stool dysbiosis.” Data were extracted synthesized using narrative review. Study quality assessed Newcastle‐Ottawa scale. Results total 1373 articles screened 14 studies selected. Significant but inconsistent changes reported. Changes observed alpha beta diversity. At phylum level, an increase Bacteroides Ascomycota, decrease Firmicutes, Actinomycetes, Basidiomycota, both Fusobacteria genus Prevotella a Faecalibacterium In post‐CCE diarrhea, decreased diversity, F/B ratio, , Phocaeicola vulgatus copri noted. For syndrome, higher abundance Proteobacteria Firmicutes/Bacteroides (F/B) ratio relative Bifidobacterium longum subsp . from controls without colonic neoplasia neoplasia, increased Candida glabrata controls, Conclusion Patients who underwent had significant dysbiosis. However, current could not detailed microbial structural functional associated CCE.

Language: Английский

---

The Ambiguous Correlation of Blautia with Obesity: A Systematic Review

Microorganisms, Journal Year: 2024, Volume and Issue: 12(9), P. 1768 - 1768

Published: Aug. 26, 2024

Obesity is a complex and multifactorial disease with global epidemic proportions, posing significant health economic challenges. Whilst diet lifestyle are well-established contributors to the pathogenesis, gut microbiota’s role in obesity development increasingly recognized. Blautia, as one of major intestinal bacteria Firmicutes phylum, reported both potential probiotic properties causal factors for different studies, making its controversial. To summarize current understanding Blautia–obesity correlation evaluate evidence from animal clinical we used “Blautia” AND “obesity” keywords searching through PubMed SpringerLink databases research articles. After removing duplicates inadequate articles using exclusion criteria, observed results between studies supporting opposing beneficial Blautia at genus level. Additionally, several showed effectiveness species level coccoides, B. wexlerae, hansenii, producta, luti. Therefore, does not demonstrate Blautia’s direct involvement pathogenic microbe or progression, which informs future therapeutic strategies targeting management.

Language: Английский

---