BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)
Published: Nov. 29, 2024
Abstract Background Cellulose is the major part of lignocellulosic biomass. It can be hydrolyzed into glucose units via specific enzymes called cellulases that have been applied in many commercial fields. There are several studies illustrate influence on apple juice clarification. However, to best our knowledge, effect microbial cellulase volatile compounds not well known. The present study aimed assess from a new bacterial isolate physicochemical properties as compounds. hydrolysis some polysaccharides (cellulose, hemicellulose, pectin) and polyphenols during production necessary reduce cloud sedimentation or color deterioration increase yield juice. So, isolates serve processing aids obtain clear with high yield. Results Cellulase-producing bacterium was isolated, characterized molecularly identified Bacillus licheniformis strain-MA1 an accession number ON840115. Optimization medium parameters implemented using Plackett–Burman design (PBd) followed by Box-Behnken (BBd) response surface methodology (RSM). PBd revealed three most important (significant) variables including carboxymethyl cellulose (CMC), corn cob, peptone had positive impact production. Additionally, agricultural residue (corn cob) strain carbon source helps reducing costs enzyme production, recycling by-products, preserving environment. optimized BBd enhanced B. 6.8-fold. A remarkable observed treated-juice sample (88.2 ± 0.15%) comparison control (75.4 0.09%). total phenolic contents cloudy clarified juices were 0.957 0.09 0.412 0.03 mg/mL, respectively. Also, DPPH FRAP assays showed antioxidant activity (Low IC 50 ) compared treatment. Twenty-seven extracted headspace solid-phase microextraction-gas analysis performed GC–MS. constituents belonged chemical classes: 15 esters; 6 alcohols; 4 aldehydes 2 acids. predominant class fraction esters sweet fruity odor. Conclusion crude obtained novel successfully clarifying agent
Language: Английский