Hindering the biofilm of microbial pathogens and cancer cell lines development using silver nanoparticles synthesized by epidermal mucus proteins from Clarias gariepinus

BMC Biotechnology, Journal Year: 2024, Volume and Issue: 24(1)

Published: May 4, 2024

Abstract Scientists know very little about the mechanisms underlying fish skin mucus, despite fact that it is a component of immune system. Fish mucus an important defence against invasive infections. Recently, and its are gaining interest among immunologists. Characterization was done on obtained silver nanoparticles Ag combined with Clarias gariepinus catfish epidermal proteins (EMP-Ag-NPs) through UV–vis, FTIR, XRD, TEM, SEM. Ag-NPs ranged in size from 4 to 20 nm, spherical form angles were 38.10°, 44.20°, 64.40°, 77.20°, Where wavelength change after formation EMP-Ag-NPs as indicate dark brown, broad band recorded at 391 nm. Additionally, antimicrobial, antibiofilm anticancer activities assessed. The present results demonstrate high activity unicellular fungi C. albicans , followed by E. faecalis . Antibiofilm showed strong both S. aureus P. aeruginosa pathogens dose-dependent manner, without affecting planktonic cell growth. Also, cytotoxicity effect investigated normal cells (Vero), breast cancer (Mcf7) hepatic carcinoma (HepG2) lines concentrations (200–6.25 µg/mL) current highly 100, 5 25 µg/mL exhibited rounding, shrinkage, deformation granulation Mcf7 HepG2 IC50 19.34 31.16 respectively while Vero appeared rounded concentration 50 shape 25, 12.5 6.25 µg/ml 35.85 µg/mL. This study evidence potential efficacy biologically generated substitute medicinal agent harmful microorganisms. Furthermore, highlights their inhibitory lines.

Language: Английский

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Biosynthesis and characterization of selenium and silver nanoparticles using Trichoderma viride filtrate and their impact on Culex pipiens

Green Processing and Synthesis, Journal Year: 2024, Volume and Issue: 13(1)

Published: Jan. 1, 2024

Abstract Some of the significant globally prevalent vector-borne illnesses are caused by Culex pipiens . Synthetic pesticides have been widely utilized to eradicate C. , which has led a number health risks for people, insect resistance, and environmental contamination. Alternative strategies therefore vitally needed. In current investigation, Trichoderma viride fungal culture filtrate was used create selenium silver nanoparticles (SeNPs AgNPs, respectively) test them on larvae in their fourth instar stage. The death rate increased significantly when SeNP AgNP concentrations increased, according results. SeNPs AgNPs affected developmental detoxification enzymes at 24 h after being treated with sublethal concentration tested NPs. As result insecticidal effect larvae, considered effective promising larvicidal agents.

Language: Английский

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Green synthesis of silver nanoparticles from Bauhinia variegata and their biological applications

Green Processing and Synthesis, Journal Year: 2024, Volume and Issue: 13(1)

Published: Jan. 1, 2024

Abstract The production of silver nanoparticles (Ag NPs) utilizing biological means with renewable resources is thought to be risk-free, environmentally benign, and safe. In this work, the capacity Bauhinia variegata produce Ag NPs was measured. Numerous methods, including UV–Vis spectroscopy, TEM, FTIR XRD, were employed for analysis produced NPs. NP antimicrobial has been examined through microtitreplate as well agar diffusion techniques. NPs’ ability scavenge free radicals at varying concentrations assessed using DPPH technique. MICs 1,000 µg·mL −1 against pathogenic microbes Staphylococcus aureus , Pseudomonas aeruginosa Candida albicans while 500 250 versus Bacillus subtilis Escherichia coli respectively. Silver showed an intriguing antioxidant capacity, achieving IC 50 46.23 μg·mL . Additionally, demonstrated possible anticancer action when applied carcinoma cell lines Caco-2, 396.2 cytotoxicity toward normal Vero 609.45 Furthermore, a range antibiofilm activities S. (MRSA). conclusion, biosynthesized via B. show promise variety safe applications.

Language: Английский

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Biosynthesis of Silver Nanoparticles from Endophytic Fungi and Their Role in Plant Disease Management

Published: Jan. 1, 2025

Language: Английский

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Myco-engineered Gold Nanoparticles and Their Employment as Biomedical Agents and Upscaling

Plasmonics, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Language: Английский

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Optimization of Phytosynthesized Zn–Se Nanocomposites via Response Surface Methodology: Characterization and Germination Impact on Gamma‐Irradiated Brassica napus L.

Microscopy Research and Technique, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

ABSTRACT The sustainable synthesis and optimization of nanocomposites (NCs) for agricultural applications remains a critical challenge. This study addresses this issue by developing Zn–Se NCs using green methods with Allium sativa Mentha arvensis plant extracts optimized via Response Surface Methodology (RSM) to achieve an average size 34.9 nm. For the first time, combined application gamma irradiation was evaluated enhance seed viability early growth in Brassica napus L. present study. results indicated that p ‐values 0.0001 0.0032 linear quadratic regression models, respectively, were significant. UV–visible spectrum showed sharp peak at 295 nm, while FTIR spectra identified functional groups acting as reducing agent, detailing their compound class, shape, intensity. Spherical synthesized effects on germination parameters assessed, revealing percentage non‐significant, ‐value > 0.05. In contrast, survival rate, seedling length, vigor index (SVI) significant, 0.002, 0.007, 0.015, respectively. demonstrated significant improvements vigor, highlighting potential nanobiofertilizer. presents novel approach extracts, achieving precise control (< 35 nm) through RSM optimization, enhancing bioavailability eco‐friendliness. recommends use plant‐mediated combination effective improve crop productivity promote eco‐friendly practices.

Language: Английский

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Biological synthesis of gold nanoparticles using endophytic fungus Cladosporium sphaerospermum (RUV07) extract: characterization and in vitro antiproliferative effect of A549 cells

Brazilian Journal of Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 26, 2025

Language: Английский

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Eco-friendly biosynthesis of silver nanoparticles using marine-derived Fusarium exquisite: optimization, characterization, and evaluation of antimicrobial, antioxidant, and cytotoxic activities

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(5)

Published: May 1, 2025

Abstract Fungi serve as efficient biocatalysts for the eco-friendly synthesis of metal nanoparticles, yielding stable and bioactive nanomaterials. In this study, silver nanoparticles were synthesized (AgNPs) using marine-derived fungus Fusarium equiseti characterized them with various analytical methods. UV–Vis spectroscopy detected a surface plasmon resonance peak at 420 nm, confirming AgNP formation, while X-ray diffraction (XRD) verified their crystalline structure. Scanning electron microscopy (SEM) transmission (TEM) revealed spherical averaging 50 nm. FTIR analysis confirmed that fungal metabolites cap stabilize AgNPs. We optimized extracellular biosynthesis 30°C, pH 8, 2 mM AgNO₃ over 72 h. The marine-adapted F. was selected its robust metabolic capacity enzyme secretion, enhancing nanoparticle stability bioactivity. Biological assessments showed these AgNPs outperformed in antimicrobial activity, minimum inhibitory concentrations (MICs) 6.5 µg/mL against Staphylococcus aureus 7.5 Escherichia coli , plus antifungal effects on Candida albicans solani . They also displayed strong antioxidant activity (IC₅₀ = 56.98 µg/mL) cytotoxicity MCF-7 breast cancer cells 24.38 µg/mL). These enhanced likely stem from acting natural capping agents, minimizing aggregation boosting bioavailability biological interactions. Molecular docking studies reinforced results, revealing binding to microbial cell wall proteins, human apoptotic regulator Bcl-2 (an anticancer target), peroxiredoxin-5 (PRDX5). This green method provides sustainable, non-toxic alternative conventional chemical approaches, avoiding hazardous reagents delivering stable, multifunctional Future vivo validation biocompatibility are planned explore clinical pharmaceutical potential -derived Graphical

Language: Английский

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Innovative Synthesis and Molecular Modeling of Actinomycetes-Derived Silver Nanoparticles for Biomedical Applications.

Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: unknown, P. 106990 - 106990

Published: Oct. 1, 2024

Language: Английский

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