Quantitative analysis of phenolics in Trifolium pratense L. flowers and evaluation of antioxidant activity by sensory DOI Creative Commons
Ramazan Erenler, İbrahim HOSAFLIOĞLU, İlyas Yıldız

et al.

Journal of New Results in Science, Journal Year: 2024, Volume and Issue: 13(2), P. 165 - 174

Published: Aug. 31, 2024

Trifolium pratense L. flowers (TPF) were collected and dried in shade this study. After extraction methanol, a diluted solution was applied to the liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) device determine bioactive compounds quantitatively. Isoquercitrin (38.64 mg/g extract), coumarin (13.66 catechin (12.52 extract) verified as major products. Antioxidant activity of TPF performed using potentiometric PVC membrane sensor evaluate 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging total phenolic content TPF. inhibited DPPH 31.4 ± 0.02% at 500-ppm concentration. However, gallic acid determined 81.43 0.07% 92.44 0.1% concentrations 12.5 ppm 25 ppm, respectively. In addition, calculated be 82.4 0.15 mg eq/g extract. It observed that has potential an antioxidant valuable source isoquercitrin, coumarin, catechin.

Language: Английский

Phytochemistry of Centaurea saligna: Silver nanoparticle synthesis, quantification of natural compounds, antioxidant and antibacterial activity DOI
Esma Nur Geçer

Published: April 3, 2025

Abstract Nanotechnology, which has widespread application areas, is a significant discipline of science. Due to the nanoparticles, many studies have been executed. Herein, silver nanoparticles (AgNPs@cs) were produced via leaves Centaurea saligna, and quantification phenolics existing in was established by LC-MS/MS. Furthermore, antioxidant antibacterial activities displayed for both extract nanoparticles. The analytical techniques, including FTIR, UV-VIS, XRD, TEM, employed elucidate structure. Moreover, stability ascertained Zeta potential. Scutellarin (8.67 mg/g extract), shikimic acid (3.97), chrysin (2.67) found as major compounds In DPPH radical scavenging activity, AgNPs@cs an excellent effect (IC50, µg/mL, 9.27 ± 0.14) association BHT (12.45 0.41, IC50, µg/mL). addition, revealed good activity against bacteria tested. absorption UV-Vis analysis at 441 nm nanoparticle formation. size calculated using TEM XRD measurements, 16.2 18.5 nm, respectively. zeta potential measurement confirmed stability. It may be possible use saligna production foods medications.

Language: Английский

Citations

0
Biofabrication of nano-silver composites from Indian catmint-Anisomeles ovata flower buds extract and evaluation of their potential in-vitro biological applications DOI

Shashiraj Kariyellappa Nagaraja,

Bidhayak Chakraborty, Hakan Anıl

et al.

Pharmacological Research - Natural Products, Journal Year: 2025, Volume and Issue: unknown, P. 100246 - 100246

Published: April 1, 2025

Language: Английский

Citations

0
Eco-friendly biosynthesis of silver nanoparticles using marine-derived Fusarium exquisite: optimization, characterization, and evaluation of antimicrobial, antioxidant, and cytotoxic activities DOI Creative Commons
Sally A. Ali, Mohamed Osman, Eslam Mohamed

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(5)

Published: May 1, 2025

Abstract Fungi serve as efficient biocatalysts for the eco-friendly synthesis of metal nanoparticles, yielding stable and bioactive nanomaterials. In this study, silver nanoparticles were synthesized (AgNPs) using marine-derived fungus Fusarium equiseti characterized them with various analytical methods. UV–Vis spectroscopy detected a surface plasmon resonance peak at 420 nm, confirming AgNP formation, while X-ray diffraction (XRD) verified their crystalline structure. Scanning electron microscopy (SEM) transmission (TEM) revealed spherical averaging 50 nm. FTIR analysis confirmed that fungal metabolites cap stabilize AgNPs. We optimized extracellular biosynthesis 30°C, pH 8, 2 mM AgNO₃ over 72 h. The marine-adapted F. was selected its robust metabolic capacity enzyme secretion, enhancing nanoparticle stability bioactivity. Biological assessments showed these AgNPs outperformed in antimicrobial activity, minimum inhibitory concentrations (MICs) 6.5 µg/mL against Staphylococcus aureus 7.5 Escherichia coli , plus antifungal effects on Candida albicans solani . They also displayed strong antioxidant activity (IC₅₀ = 56.98 µg/mL) cytotoxicity MCF-7 breast cancer cells 24.38 µg/mL). These enhanced likely stem from acting natural capping agents, minimizing aggregation boosting bioavailability biological interactions. Molecular docking studies reinforced results, revealing binding to microbial cell wall proteins, human apoptotic regulator Bcl-2 (an anticancer target), peroxiredoxin-5 (PRDX5). This green method provides sustainable, non-toxic alternative conventional chemical approaches, avoiding hazardous reagents delivering stable, multifunctional Future vivo validation biocompatibility are planned explore clinical pharmaceutical potential -derived Graphical

Language: Английский

Citations

0
Quantitative analysis of phenolics in Trifolium pratense L. flowers and evaluation of antioxidant activity by sensory DOI Creative Commons
Ramazan Erenler, İbrahim HOSAFLIOĞLU, İlyas Yıldız

et al.

Journal of New Results in Science, Journal Year: 2024, Volume and Issue: 13(2), P. 165 - 174

Published: Aug. 31, 2024

Trifolium pratense L. flowers (TPF) were collected and dried in shade this study. After extraction methanol, a diluted solution was applied to the liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) device determine bioactive compounds quantitatively. Isoquercitrin (38.64 mg/g extract), coumarin (13.66 catechin (12.52 extract) verified as major products. Antioxidant activity of TPF performed using potentiometric PVC membrane sensor evaluate 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging total phenolic content TPF. inhibited DPPH 31.4 ± 0.02% at 500-ppm concentration. However, gallic acid determined 81.43 0.07% 92.44 0.1% concentrations 12.5 ppm 25 ppm, respectively. In addition, calculated be 82.4 0.15 mg eq/g extract. It observed that has potential an antioxidant valuable source isoquercitrin, coumarin, catechin.

Language: Английский

Citations

0