Synergistic anticancer potential of biogenic nanoparticles and cryptomeridiol from Sphaeranthus indicus: targeting gastric cancer through apoptosis and cell cycle arrest DOI Creative Commons
Pan Zhao, Jigang Wang, Chang Zou

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 3, 2025

S. indicus has demonstrated promising therapeutic potential due to its bioactive compounds. This study investigated the cytotoxic and pro-apoptotic effects of Sphaeranthus extract, active fraction, biosynthesized silver nanoparticles (AgNPs) on human gastric cancer (HGT-1) cells. The plant was collected subjected Soxhlet extraction using ethanol, followed by sequential solvent partitioning silica gel column chromatography isolate fractions. Green synthesis AgNPs conducted extract. Structural characterization via UV- spectroscopy, FTIR, XRD, FESEM. Cytotoxicity assessed MTT CCK-8 assays, while apoptosis induction evaluated through qPCR Western blot analysis key apoptotic markers. ethanolic extract exhibited moderate cytotoxicity against HGT-1 cells, whereas enhanced anticancer activity with reduced toxicity normal hepatocytes. identified as cryptomeridiol, showed highest selectivity potency revealed significant upregulation p21 downregulation CDK2, suggesting cell cycle arrest. confirmed increased expression caspase-3 caspase-9 a reduction in XIAP, indicating activation. underscores compounds agents, particularly cancer. findings provide basis for further exploration into their mechanism action broader pharmacological applications. Keywords: indicus, Bioactive compounds, Silver (AgNPs), Gastric cancer-HGT-1, Apoptosis.

Language: Английский

Synergistic anticancer potential of biogenic nanoparticles and cryptomeridiol from Sphaeranthus indicus: targeting gastric cancer through apoptosis and cell cycle arrest DOI Creative Commons
Pan Zhao, Jigang Wang, Chang Zou

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 3, 2025

S. indicus has demonstrated promising therapeutic potential due to its bioactive compounds. This study investigated the cytotoxic and pro-apoptotic effects of Sphaeranthus extract, active fraction, biosynthesized silver nanoparticles (AgNPs) on human gastric cancer (HGT-1) cells. The plant was collected subjected Soxhlet extraction using ethanol, followed by sequential solvent partitioning silica gel column chromatography isolate fractions. Green synthesis AgNPs conducted extract. Structural characterization via UV- spectroscopy, FTIR, XRD, FESEM. Cytotoxicity assessed MTT CCK-8 assays, while apoptosis induction evaluated through qPCR Western blot analysis key apoptotic markers. ethanolic extract exhibited moderate cytotoxicity against HGT-1 cells, whereas enhanced anticancer activity with reduced toxicity normal hepatocytes. identified as cryptomeridiol, showed highest selectivity potency revealed significant upregulation p21 downregulation CDK2, suggesting cell cycle arrest. confirmed increased expression caspase-3 caspase-9 a reduction in XIAP, indicating activation. underscores compounds agents, particularly cancer. findings provide basis for further exploration into their mechanism action broader pharmacological applications. Keywords: indicus, Bioactive compounds, Silver (AgNPs), Gastric cancer-HGT-1, Apoptosis.

Language: Английский

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