Unveiling the potency of ZnO and CuO nanocomposites in combating hepatocellular carcinoma by inducing cell death and suppressing migration DOI Creative Commons
Rasha Mahmoud Allam,

Nesma M. E. Abo El-Nasr,

Marawan A. Elbaset

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: May 3, 2025

Abstract Human hepatocellular carcinoma (HCC) is recognized as one of the leading causes death globally and resistant to several anticancer drugs. As a result, it critical identify more effective druggable therapies. Metal oxide nanoparticles (MO-NPs), especially nanocomposites, have recently received much attention owing their potential applications in cancer therapy. In this study, we synthesized zinc (ZnO) copper (CuO) nanocomposites different ratios (N1, N2, N3). We evaluated cytotoxicity against two HCC cell lines (HepG2 HuH-7) normal liver (BNL), compared with Sorafenib standard Then, investigated underlying mechanisms action employing flow cytometry, migration assay, western blot. The results showed that nanocomposite an equal ratio both ZnO CuO-NPs (N1) exhibited highest cytotoxic activity on HuH7 line while exerting no detrimental impact rat epithelial cells. Further investigation into toxicity N1 revealed three modalities induced (apoptotic, necrotic, autophagic) along S- G2/M cycle arrest, suggesting mitotic catastrophe. Furthermore, displayed potent anti-migratory activity, surpassing sorafenib, upregulated protein level autophagy marker beclin-1, downregulated EMT-marker vimentin. Overall, our findings combining ZnO-NPs intriguing combating HCC, providing prospective guidance for evolving therapy bimetallic NPs.

Language: Английский

Microplastics – A Growing Concern as Carcinogens in Cancer Etiology: Emphasis on Biochemical and Molecular Mechanisms DOI
Naveen Kumar,

Mridul Lamba,

Ashok Kumar Pachar

et al.

Cell Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: 82(4), P. 3109 - 3121

Published: July 20, 2024

Language: Английский

Citations

12
Pharmacological Insights: Mitochondrial ROS Generation by FNC (Azvudine) in Dalton’s Lymphoma Cells Revealed by Super Resolution Imaging DOI
Naveen Kumar,

Vikram Delu,

Ilya V. Ulasov

et al.

Cell Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: 82(2), P. 873 - 883

Published: March 14, 2024

Language: Английский

Citations

6
Fluorescent tetrazolylpyrene unnatural nucleoside in sensing BSA protein DOI
Subhendu Sekhar Bag, Hiranya Gogoi, Sujata Roy

et al.

Journal of Photochemistry and Photobiology A Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 116290 - 116290

Published: Jan. 1, 2025

Citations

0
Oxadiazole-2-thione based Co(II) Complexes as Antiproliferative Agents: Synthesis and Structural Characterization DOI

Ajay K. Vishwakarma,

Alok Shukla,

Sankatha P Sonkar

et al.

Journal of Molecular Structure, Journal Year: 2025, Volume and Issue: unknown, P. 141781 - 141781

Published: Feb. 1, 2025

Language: Английский

Citations

0
An electrochemical detection method for polyvinyl chloride microplastics in water bodies DOI
Özge Sürücü

Journal of Molecular Structure, Journal Year: 2025, Volume and Issue: unknown, P. 142086 - 142086

Published: March 1, 2025

Language: Английский

Citations

0
Potent cytotoxicity and induction of ROS-mediated genomic instability, mitochondrial dysfunction, and apoptosis by Y2O3 NPs in Hep-G2 hepatic cancer cells DOI Creative Commons
Hanan R. H. Mohamed,

Rawan Essam,

Basma A. Mohamed

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Abstract Hepatic cancer, one of the most prevalent and lethal cancers globally, remains a significant health challenge, with limited treatment options underscoring urgent need for novel, more effective therapies. Yttrium oxide nanoparticles (Y 2 O 3 NPs) have attracted attention in nanomedicine due to their promising properties, including enhanced drug delivery, imaging capabilities, therapeutic effects. However, specific impact Y NPs on hepatic cancer is largely unexplored. Therefore, this study was conducted assess cytotoxic effects cell viability, reactive oxygen species (ROS) generation, genomic stability, mitochondrial integrity, apoptosis induction Hep-G2 cells. The results from SRB cytotoxicity assay demonstrated strong concentration-dependent decrease notably low half-maximal inhibitory concentration (IC50) value 13.15 µg/ml. Exposure IC50 led increased ROS DNA damage induction, loss membrane potential. Furthermore, expression pro-apoptotic p53 ND3 genes significantly upregulated, while anti-apoptotic Bcl-2 gene markedly downregulated, triggering cells after 72 h exposure NPs. Collectively, these findings highlight potential emphasizing further research fully explore efficacy as option liver cancer.

Language: Английский

Citations

0
Antitumor potential of ivermectin against T-cell lymphoma-bearing hosts DOI
Alok Kumar Shukla,

Arpit Sharma,

Shivani Gupta

et al.

Medical Oncology, Journal Year: 2025, Volume and Issue: 42(5)

Published: April 21, 2025

Language: Английский

Citations

0
Unveiling the potency of ZnO and CuO nanocomposites in combating hepatocellular carcinoma by inducing cell death and suppressing migration DOI Creative Commons
Rasha Mahmoud Allam,

Nesma M. E. Abo El-Nasr,

Marawan A. Elbaset

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: May 3, 2025

Abstract Human hepatocellular carcinoma (HCC) is recognized as one of the leading causes death globally and resistant to several anticancer drugs. As a result, it critical identify more effective druggable therapies. Metal oxide nanoparticles (MO-NPs), especially nanocomposites, have recently received much attention owing their potential applications in cancer therapy. In this study, we synthesized zinc (ZnO) copper (CuO) nanocomposites different ratios (N1, N2, N3). We evaluated cytotoxicity against two HCC cell lines (HepG2 HuH-7) normal liver (BNL), compared with Sorafenib standard Then, investigated underlying mechanisms action employing flow cytometry, migration assay, western blot. The results showed that nanocomposite an equal ratio both ZnO CuO-NPs (N1) exhibited highest cytotoxic activity on HuH7 line while exerting no detrimental impact rat epithelial cells. Further investigation into toxicity N1 revealed three modalities induced (apoptotic, necrotic, autophagic) along S- G2/M cycle arrest, suggesting mitotic catastrophe. Furthermore, displayed potent anti-migratory activity, surpassing sorafenib, upregulated protein level autophagy marker beclin-1, downregulated EMT-marker vimentin. Overall, our findings combining ZnO-NPs intriguing combating HCC, providing prospective guidance for evolving therapy bimetallic NPs.

Language: Английский

Citations

0