LINC00704 facilitates cell proliferation, migration, and invasion via miR-323a-3p/SLC44A1 axis in epithelial ovarian cancer

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 29, 2025

Epithelial ovarian cancer (EOC) is the most common subtype of and a highly recurrent lethal malignancy female reproductive system. Accumulating evidence has indicated that long non-coding RNAs (lncRNAs) play pivotal roles in tumorigenesis. Long intergenic non-protein coding RNA 704 (LINC00704) been recognized as an oncogenic lncRNA several malignancies. However, its specific role EOC remains unclear. In this study, RT-qPCR revealed upregulation LINC00704 tissues cell lines. CCK-8 EdU assays showed knockdown inhibited proliferation. Flow cytometry analysis demonstrated silencing induced cycle arrest apoptosis. Transwell inhibitory effects on migration invasion. Mechanistically, bioinformatics analysis, pull-down, luciferase reporter immunoprecipitation upregulated SLC44A1 expression by competitively binding to miR-323a-3p. Moreover, rescue experiments overexpression could reverse silencing-mediated malignant behaviors cells. conclusion, promotes aggressiveness vitro regulating miR-323a-3p/SLC44A1 axis.

Language: Английский

NRF1-Induced lncRNA DDX11-AS1 Contributes to the Progression of Hepatocellular Carcinoma via Activating CA9 Expression and the MEK/ERK Pathway

Journal of Hepatocellular Carcinoma, Journal Year: 2025, Volume and Issue: Volume 12, P. 891 - 908

Published: May 1, 2025

DDX11 antisense RNA 1 (DDX11-AS1) has been recognized for its strong correlation with hepatocellular carcinoma (HCC). Nevertheless, the exact biological functions and fundamental molecular processes of DDX11-AS1 in HCC require further in-depth investigation. A comprehensive bioinformatics analysis was carried out to explore expression clinical implication utilizing TCGA data. qRT-PCR employed validate tissues/cell lines. fluorescence situ hybridization (RNA-FISH) used observe subcellular localization cells. Loss-of-function experiments, both vitro vivo, were executed elucidate HCC. sequencing (RNA-seq) identify genes signaling pathways potentially regulated by DDX11-AS1. Rescue experiments conducted that carbonic anhydrase IX (CA9) mediates promoting progression. The influence nuclear respiratory factor (NRF1) on transcription investigated through dual-luciferase reporter assays ChIP-qPCR. increased is positively associated several aggressive characteristics (pathologic T stage, histologic grade, AFP level, vascular invasion), closely linked unfavorable outcomes patients, acting as a separate hazardous overall survival. predominantly situated nucleus knockdown impeded growth, migration, invasion capabilities cells vitro, reduced tumor enlargement subcutaneous mouse model. RNA-Seq unveiled silencing lessened CA9 suppressed activity MEK/ERK cascade uncovered acts downstream target facilitating cancer-causing roles Furthermore, revealed be transcriptionally NRF1. DDX11-AS1, NRF1-induced lncRNA, facilitates development upregulating activating cascade.

Language: Английский

Global research status and frontiers on ferroptosis in hepatocellular carcinoma: a comprehensive bibliometric and visualized analysis

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: May 2, 2025

Objective The purpose of this study was to analyze the research hotspots and future trends in field ferroptosis hepatocellular carcinoma past 10 years by using bibliometrics visualization software, provide reference for directions field. Methods Web Science database searched from January 1, 2012, October 30, 2024, annual publication volume, countries, institutions, journals, authors, references, keywords, other information were analyzed bibliometrics, VOSviewer, CiteSpace. Results A total 645 English articles 729 institutions 32 countries included study, with 4545 authors published 261 journals. In three years, 518 published, accounting 80.3%. China has most publications, followed United States. Frontiers Oncology had highest number papers (n=26), while Cell citations (n=1206). current mainly focuses on two aspects: one is mechanism explore new therapeutic targets, exploration methods, such as photodynamic therapy nanomaterials, order inhibit proliferation tumor cells, reduce drug resistance, enhance efficacy regulating ferroptosis, which may become a development trend. Conclusion recent there have been increasing studies association between carcinoma. This first comprehensive bibliometric provides reliable promotes its further development.

Language: Английский