A novel duplex qPCR-HRMA technique for simultaneous detection of Listeria monocytogenes and Salmonella typhimurium in meat products

Food Chemistry, Journal Year: 2025, Volume and Issue: 474, P. 143245 - 143245

Published: Feb. 5, 2025

Language: Английский

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Meat quality, safety, dietetics, environmental impact, and alternatives now and ten years ago: a critical review and perspective

Food Production Processing and Nutrition, Journal Year: 2025, Volume and Issue: 7(1)

Published: Feb. 13, 2025

Abstract Meat consumption is growing steadily. As with any research, meat investigation requires an overall view of the study field to identify current directions and reveal prospective trends. The number publications on research steadily reaching several thousand per year. This creates difficulties in covering all available information forces researchers increasingly limit themselves narrow issues their direction. We analysed main trends published recently ten years ago. identified areas based abstracts articles word “meat” title Web Science database time intervals 2000–2003, 2010–2013 2020–2023. also mapped terms from directly related using VOSviewer OpenAlex application programming interface. Among selected dominant Science, were systematised reviews: 1182 2013 2610 2023. Such increase indicates a sharp rise interest topic existence questions that need be resolved. Therefore, overview 2023 was presented. Research declining share actively developing identified, unresolved pressing revealed changes demonstrate shift microbiology technology obtaining products towards methods development, problems nutrition, global warming. In conclusion, prospects for these have been considered. regulate negative effects production justifies rationality interdisciplinary approaches integrating environmental, health, ethical perspectives. most promising further are rationale strategies reduce consumption. Graphical

Language: Английский

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Differential detection of sheep and goat meat using duplex real-time PCR and high-resolution melt analysis

Food Chemistry Advances, Journal Year: 2023, Volume and Issue: 2, P. 100309 - 100309

Published: May 9, 2023

Authenticity in meat and products is of paramount importance preventing adulteration ensuring food safety. Since morphologically indistinct samples like sheep goat requires accurate sensitive differentiation, we developed validated a duplex real-time polymerase chain reaction (qPCR) assay with high-resolution melt analysis (HRMA) for simultaneous identification meat. Species-specific amplicons melting temperature 78 °C 80 (with associated standard errors) was identified. The optimized basic PCR parameters the HRMA detection standardized based on curve shape, color inflexion points. absolute limit (LODabs) quantification (LOQ) were found to be 0.39 ng 0.78 target DNA respectively. A sensitivity 0.1% obtained both relative (LODrel) also 5%. linearity efficiency evaluated simplex assays. applicability using proficiency test commercially available raw processed samples. results showed that this closed-tube qPCR-HRMA method can rapid technique speciation.

Language: Английский

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Authentication of traditional meat products (Haleem) sold in India: a first report confirming species mislabelling

Journal of Food Science and Technology, Journal Year: 2024, Volume and Issue: 61(5), P. 1003 - 1012

Published: March 5, 2024

Language: Английский

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Evaluation of Antimicrobial Activity by Marine Nocardiopsis dassonvillei against Foodborne Listeria monocytogenes and Shiga Toxin-Producing Escherichia coli

Microorganisms, Journal Year: 2023, Volume and Issue: 11(10), P. 2539 - 2539

Published: Oct. 12, 2023

The emergence of multidrug-resistant pathogens creates public health challenges, prompting a continuous search for effective novel antimicrobials. This study aimed to isolate marine actinomycetes from South Africa, evaluate their in vitro antimicrobial activity against Listeria monocytogenes and Shiga toxin-producing Escherichia coli, characterize mechanisms action. Marine were isolated identified by 16S rRNA sequencing. Gas chromatography-mass spectrometry (GC-MS) was used identify the chemical constituents bioactive actinomycetes' secondary metabolites. Antibacterial metabolites assessed broth microdilution method, mode actions predicted using computational docking. While five strains showed antibacterial during primary screening, only Nocardiopsis dassonvillei strain SOD(B)ST2SA2 exhibited screening activity. GC-MS major compounds: 1-octadecene, diethyl phthalate, pentadecanoic acid, 6-octadecenoic trifluoroacetoxy hexadecane. SOD(B)ST2SA2's extract demonstrated minimum inhibitory concentration bactericidal concentration, ranging 0.78-25 mg/mL 3.13 > 25 mg/mL, respectively. Diethyl phthalate displayed lowest bacterial protein-binding energies (kcal/mol): -7.2, dihydrofolate reductase; -6.0, DNA gyrase B; -5.8, D-alanine:D-alanine ligase. Thus, N. is potentially good source compounds that can be control STEC monocytogenes.

Language: Английский

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Evaluation of Antimicrobial Activity by Marine <em>Nocardiopsis dassonvillei </em>against Foodborne <em>Listeria monocytogenes</em> and Shiga Toxin-Producing <em>Escherichia coli</em>

Published: Aug. 15, 2023

The emergence of multidrug-resistant pathogens such as Listeria monocytogenes and Shiga toxin-producing Escherichia coli (STEC) poses public health challenges, this has led to a continuous search for effective novel antimicrobial agents. This study aimed isolate marine actinomycetes from South Africa, evaluate their in vitro activity against STEC, characterize mechanisms action. Marine actinoymcetes were isolated identified by 16S rRNA sequencing. Gas chromatography-mass spectrometry (GC-MS) was used identify the chemical constituents bioactive secondary metabolites. Antibacterial metabolites assessed broth microdilution method mode actions predicted using computational docking. While five strains showed antibacterial during primary screening, only Nocardiopsis dassonvillei strain SOD(B)ST2SA2 exhibited screening activity. GC-MS major compounds: 1-octadecene, diethyl phthalate, pentadecanoic acid, 6-octadecenoic tri-fluoroacetoxy hexadecane. SOD(B)ST2SA2`s extract demonstrated minimum inhibitory con-centration bactericidal concentration ranging 0.78–25 mg/ml 3.13 &gt; 25 mg/ml, respectively. Diethyl phthalate displayed lowest bacterial protein binding energies (kcal/mol): −7.2, dihydrofolate reductase; −6.0, DNA gyrase B, −5.8, D-alanine:D-alanine lig-ase. Thus, N. is potentially good source compounds that can be control STEC monocytogenes.

Language: Английский

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Hybrid Nucleic Acid Detection Approach for Ion Concentration Polarization Enabled Polymerase Chain Reaction with Reduced Cycle Numbers

Published: Jan. 1, 2024

Polymerase chain reaction (PCR) plays important roles in the detection and quantification of nucleic acid targets. To cut-off time, also to reduce any potential by-products during thermal cycles, it is required keep cycle numbers as few possible. Here, this work, a hybrid PCR microfluidic device proposed perform test with only 25 which are much lower than 35 or 40 cycles standard operations. With novel design, target molecules were amplified by avoid by-products. And then actively enriched ion concentration polarization (ICP) effects increase local significantly. Owe function "amplification active concentration", PCR- ICP enables more credible allows take faster less cyclings. As demonstration, meat adulteration experiments taken show sensitivity limit device, verifying performance method.

Language: Английский

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Differential Expression of Skeletal Muscle Sites and Fast and Slow Muscle Fibers in Mongolian Horses

Russian Journal of Genetics, Journal Year: 2024, Volume and Issue: 60(11), P. 1504 - 1515

Published: Nov. 1, 2024

Language: Английский

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