Tandem Mass Spectrometry Imaging to Assess Illudin S Distributions in Omphalotus Illudens Mushrooms DOI
Kōichi Inoue,

Shigeki Matsui,

Takahiro Takayama

et al.

Published: Jan. 1, 2024

Poisoning metabolites within mushrooms typically exert varying physiological and biological effects; however, their distributions are seldom investigated. This study focused on imaging illudin S, a representative poisoning metabolite Omphalotus illudens mushroom, using desorption electrospray ionization–tandem mass spectrometry (DESI–MS/MS) coupled with liquid chromatography–electrospray (LC–ESI–MS/MS). Initially, LC–ESI–MS/MS conditions were established for the selective detection of S (m/z 247→159, positive mode) from extracted mushroom. Based this MS/MS condition, each mushroom was divided into nine sections, section subjected to specific DESI–MS/MS mode (pixel size: 200 × μm). The results revealed high concentrations in stalks growing trees. Further, re-evaluations LC–MS/MS assay seven-fold difference between stalk other regions. marks first attempt at assessing toxic poisonous mushrooms, thus offering valuable insights including efficient utilization analogs applications practices mitigating food risks.

Language: Английский

New findings in metabolomics in food mycology DOI Creative Commons
Marta Sousa Silva, Carlos Cordeiro

Current Opinion in Food Science, Journal Year: 2024, Volume and Issue: 57, P. 101175 - 101175

Published: May 10, 2024

In the domain of food, fungi exhibit a dual role: while some enhance flavour and texture others cause spoilage contamination, posing risks to human health through mycotoxins. Fungi leave their unique chemical signature on foods they colonize synthesis primary secondary metabolites. Metabolomics, focusing study small molecules present in biological systems, provides insights into fungal metabolic processes impact food quality safety. This review explores recent advancements metabolomics for mycology, highlighting its applications understanding interactions with substrates. Moreover, future directions this area are discussed.

Language: Английский

Citations

4
Mass spectrometry imaging in plants, microbes, and food: a review DOI Creative Commons

Mudita Vats,

Berta Cillero‐Pastor, Eva Cuypers

et al.

The Analyst, Journal Year: 2024, Volume and Issue: 149(18), P. 4553 - 4582

Published: Jan. 1, 2024

Plant health, which affects the nutritional quality and safety of derivative food products, is influenced by symbiotic interactions with microorganisms. These influence local molecular profile at tissue level. Therefore, studying distribution molecules within plants, microbes, plant-based crucial to assess plant ensure agricultural products that become part our supply, plan management practices. Within this framework, samples can be visualized mass spectrometry imaging (MSI). This review describes key MSI methodologies, highlighting role they play in unraveling localization metabolites, lipids, proteins, pigments, elemental components across products. Furthermore, investigations involve multimodal approaches combining other techniques are described. The advantages limitations different their applicability diverse agro-food studies described enable informed choices for tailored analyses. For example, some technologies meticulous sample preparation while others compromise spatial resolution gain throughput. Key parameters such as sensitivity, ionization bias fragmentation, reference database compound class specificity discussed review. With ongoing refinements instrumentation, data analysis, integration complementary techniques, deepens insight into biology ecosystem. turn empowers quest sustainable productive

Language: Английский

Citations

4
Promising mass spectrometry imaging: exploring microscale insights in food DOI
Xinyu Cao,

Peixu Cong,

Yu Song

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 32

Published: Jan. 16, 2025

This review focused on mass spectrometry imaging (MSI), a powerful tool in food analysis, covering its ion source schemes and procedures their applications quality, safety, nutrition to provide detailed insights into these aspects. The presented introduction both commonly used emerging ionization sources, including nanoparticle laser desorption/ionization (NPs-LDI), air flow-assisted (AFAI), desorption with through-hole alumina membrane (DIUTHAME), plasma-assisted (PALDI), low-temperature plasma (LTP). In the MSI process, particular emphasis was placed quantitative (QMSI) super-resolution algorithms. These two aspects synergistically enhanced MSI's analytical capabilities: QMSI enabled accurate relative absolute quantification, providing reliable data for composition while algorithms improved molecular spatial resolution, facilitating biomarker trace substance detection. outperformed conventional methods comprehensively exploring functional factors, discovery, monitoring processing/storage effects by discerning species distributions. However, challenges such as immature techniques, complex processing, non-standardized instruments, high costs existed. Future trends instrument enhancement, multispectral integration, analysis improvement were expected deepen our understanding of chemistry highlighting revolutionary potential research.

Language: Английский

Citations

0
Recent advances in metabolomic research within food mycology DOI Creative Commons

Wei-Feng HE

Journal of Food Protection, Journal Year: 2025, Volume and Issue: unknown, P. 100494 - 100494

Published: March 1, 2025

In food science, fungi demonstrate a paradoxical role: some species contribute positively by improving food's flavor, aroma, and texture, while others undermine safety quality, leading to spoilage or contamination through the production of harmful mycotoxins. When colonize materials, they produce distinct chemical profiles consisting both primary secondary metabolites. Metabolomics, field dedicated analysis small molecular compounds within biological entities, offers valuable insights into fungal metabolic activities their impact on quality. This paper explores recent progress in applying metabolomics investigate systems, focusing complex interactions between environments. Additionally, it identifies promising directions for advancing research this rapidly evolving domain.

Language: Английский

Citations

0
Quality Control Standards for Batch Effect Evaluation and Correction in Mass Spectrometry Imaging DOI Creative Commons
Luojiao Huang,

Y. Kim,

Benjamin Balluff

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 12, 2025

Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) allows spatial molecular profiling. Despite many successful applications, an appropriate control of technical variations is still lacking for result reproducibility assessment and maximizing the MSI data quality. To address this, we introduce a novel quality standard (QCS) design analysis pipeline accounting variability due to sample preparation instrument performance. Firstly, created tissue mimicking QCS consisting propranolol in gelatin matrix. We showed that this mimics ion suppression tissue. Next, three-day batch experiment demonstrated QCS's performance longitudinal variations, establishing it as effective indicator effects. Then three computational approaches effect correction were applied first time MALDI-MSI data, leading significant reduction variation improved clustering by using multivariate principal component analysis. Altogether, offer designed combination with users evaluation correction.

Language: Английский

Citations

0
Tandem mass spectrometry imaging to assess illudin S distributions in Omphalotus illudens mushrooms DOI Creative Commons

Shigeki Matsui,

Takahiro Takayama, Kōichi Inoue

et al.

Talanta Open, Journal Year: 2024, Volume and Issue: 10, P. 100335 - 100335

Published: June 4, 2024

Poisoning metabolites within mushrooms typically exert varying physiological and biological effects; however, their distributions are seldom investigated. This study focused on imaging illudin S, a representative poisoning metabolite Omphalotus illudens mushroom, using desorption electrospray ionization–tandem mass spectrometry (DESI–MS/MS) coupled with liquid chromatography–electrospray (LC–ESI–MS/MS). Initially, LC–ESI–MS/MS conditions were established for the selective detection of S (m/z 247→159, positive mode) from extracted mushroom. Based this MS/MS condition, each mushroom was divided into nine sections, section subjected to specific DESI–MS/MS mode (pixel size: 200 × μm). The results revealed high concentrations in stalks growing trees. Further, re-evaluations LC–MS/MS assay seven-fold difference between stalk other regions. marks first attempt at assessing toxic poisonous mushrooms, thus offering valuable insights including efficient utilization analogs applications.

Language: Английский

Citations

1
Tandem Mass Spectrometry Imaging to Assess Illudin S Distributions in Omphalotus Illudens Mushrooms DOI
Kōichi Inoue,

Shigeki Matsui,

Takahiro Takayama

et al.

Published: Jan. 1, 2024

Poisoning metabolites within mushrooms typically exert varying physiological and biological effects; however, their distributions are seldom investigated. This study focused on imaging illudin S, a representative poisoning metabolite Omphalotus illudens mushroom, using desorption electrospray ionization–tandem mass spectrometry (DESI–MS/MS) coupled with liquid chromatography–electrospray (LC–ESI–MS/MS). Initially, LC–ESI–MS/MS conditions were established for the selective detection of S (m/z 247→159, positive mode) from extracted mushroom. Based this MS/MS condition, each mushroom was divided into nine sections, section subjected to specific DESI–MS/MS mode (pixel size: 200 × μm). The results revealed high concentrations in stalks growing trees. Further, re-evaluations LC–MS/MS assay seven-fold difference between stalk other regions. marks first attempt at assessing toxic poisonous mushrooms, thus offering valuable insights including efficient utilization analogs applications practices mitigating food risks.

Language: Английский

Citations

0