Biological and molecular studies of phytoconstituents from Hordeum vulgare L. leaves: potential treatment for prostate (DU-145) and skin cancer (B16-F10) cell line DOI Creative Commons
Kiran Kangra, Somdutt Mujwar, Shruti Kakkar

et al.

Beni-Suef University Journal of Basic and Applied Sciences, Journal Year: 2025, Volume and Issue: 14(1)

Published: May 6, 2025

Abstract Barley, scientifically known as Hordeum vulgare ( H. ), is a versatile cereal grain that belongs to the family Poaceae. Barley has exhibited several pharmacological activities, including anti-diuretic, anti-malarial, anti-rheumatic, anti-dysentery, anti-diarrhoea, and in treatment of gastrointestinal disorders. The objective this study was assess antioxidant, anti-inflammatory, cytotoxic properties leaves vulgare. Eight extracts were prepared by Soxhlation maceration method using solvents like petroleum ether, chloroform, hydro-alcohol, water. antioxidant activity analysed through 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, while anti-inflammatory assessed protein denaturation, anti-protease, anti-lipoxygenase assays. Extracts from showed maximum so they further screened for cytotoxicity against skin prostate cancer cell lines. Chloroform extract (HSC) prominent compared cancer. To identify phytoconstituents present HSC extract, an analysis conducted high-performance thin-layer chromatography (HPTLC) gas chromatography–mass spectrometry (GC–MS) techniques. revealed presence 16 different compounds extract. Subsequently, all underwent silico modelling investigate their Notably, octadecenoic acid emerged most compound terms its efficacy inhibiting mitogen-activated kinase (MAPK). Molecular docking simulation studies carried out followed dynamic confirm thermodynamic stability. results strong binding affinity MAPK which concludes potential inhibitor MAP used

Language: Английский

Biological and molecular studies of phytoconstituents from Hordeum vulgare L. leaves: potential treatment for prostate (DU-145) and skin cancer (B16-F10) cell line DOI Creative Commons
Kiran Kangra, Somdutt Mujwar, Shruti Kakkar

et al.

Beni-Suef University Journal of Basic and Applied Sciences, Journal Year: 2025, Volume and Issue: 14(1)

Published: May 6, 2025

Abstract Barley, scientifically known as Hordeum vulgare ( H. ), is a versatile cereal grain that belongs to the family Poaceae. Barley has exhibited several pharmacological activities, including anti-diuretic, anti-malarial, anti-rheumatic, anti-dysentery, anti-diarrhoea, and in treatment of gastrointestinal disorders. The objective this study was assess antioxidant, anti-inflammatory, cytotoxic properties leaves vulgare. Eight extracts were prepared by Soxhlation maceration method using solvents like petroleum ether, chloroform, hydro-alcohol, water. antioxidant activity analysed through 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, while anti-inflammatory assessed protein denaturation, anti-protease, anti-lipoxygenase assays. Extracts from showed maximum so they further screened for cytotoxicity against skin prostate cancer cell lines. Chloroform extract (HSC) prominent compared cancer. To identify phytoconstituents present HSC extract, an analysis conducted high-performance thin-layer chromatography (HPTLC) gas chromatography–mass spectrometry (GC–MS) techniques. revealed presence 16 different compounds extract. Subsequently, all underwent silico modelling investigate their Notably, octadecenoic acid emerged most compound terms its efficacy inhibiting mitogen-activated kinase (MAPK). Molecular docking simulation studies carried out followed dynamic confirm thermodynamic stability. results strong binding affinity MAPK which concludes potential inhibitor MAP used

Language: Английский

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