Integrated Transcriptomic and Metabolomic Analysis of G. hirsutum and G. barbadense Responses to Verticillium Wilt Infection DOI Open Access

Baoguang Xing,

Pengtao Li,

Yanfang Li

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 28 - 28

Published: Dec. 24, 2024

Verticillium wilt (VW) caused by dahliae (Vd) is a devastating fungal cotton disease characterized high pathogenicity, widespread distribution, and frequent variation. It leads to significant losses in both the yield quality of cotton. Identifying key non-synonymous single nucleotide polymorphism (SNP) markers crucial genes associated with VW resistance Gossypium hirsutum barbadense, subsequently breeding new disease-resistant varieties, are essential for management. Here, we sequenced transcriptome metabolome roots TM-1 (G. hirsutum) Hai7124 barbadense) after 0, 1, 2 days V991 inoculation. Transcriptome analysis identified total 72,752 genes, 5814 differentially expressed (DEGs) determined through multiple group comparisons. KEGG enrichment revealed that pathways enriched DEGs obtained from longitudinal transverse comparisons contained glutathione metabolism pathway. Metabolome 995 metabolites, 22 accumulated metabolites (DAMs), which were correlated including metabolism, degradation valine, leucine, isoleucine, biosynthesis terpenoids, alkaloids, pyridine, piperidine. The conjoint transcriptomic metabolomic sequencing DAMs pathway, candidate gene GH_D11G2329 (glutathione S-transferase, GSTF8) potentially response infection was selected. These findings establish basis investigating mechanisms underlying plant’s VW.

Language: Английский

Genetic diversity and population structure analysis of short‐day onions using molecular markers in association with resistance to Fusarium basal rot DOI Open Access

SaeidReza Poursakhi,

Hossein Ali Asadi‐Gharneh, Mehdi Nasr‐Esfahani

et al.

Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(1)

Published: Jan. 1, 2025

Abstract In this research, we analyzed Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeats (ISSR) and Sequence‐related amplified polymorphism (SRAP) markers to evaluate the genetic diversity of eighteen different onion genotypes with various resistant levels FOC . The results showed that means between RAPD primers was 61.11 81.81%; ISSR primers, 62.50 SRAP 56.25 76.25%. Overall, by assessing MI, PIC, I H indices, indicating best thrive in evaluating related populations. There is a significant correlation generated dendrograms based on similarity matrices. classification pattern shows corresponding disease severity bunches. So all three studied, ‘Saba’ ‘Saba ‐ HS’, most ones disease, were grouped branch, ‘Sahar HS’ ‘Golden Eye’, susceptible also another branch separately. This finding indicates predominant act as linked resistance gene(s) against , which can be used select onions any breeding scheme.

Language: Английский

Citations

1
Recent Advances in Molecular Genetics of Onion DOI Creative Commons
Suman Sharma, Anil Khar, Jiffinvir Singh Khosa

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(3), P. 256 - 256

Published: March 7, 2024

Onion is an important vegetable crop because it adds nutritional value and diversity to food preparation. Understanding recent advancements in onion molecular genetics essential improve production, quality, disease resistance. Cutting-edge genomic technologies like genetic mapping RNA sequencing reveal genes pathways. The review examines the progress utilizing various markers study divergence. exploration extends understanding pathways responsible for bulb color chemical composition factors influencing bulbing, flowering, vernalization. Additionally, article explores quantitative trait loci associated with resistance major damaging diseases delves into role of different male sterility hybrid development. publication whole genome sequence onions will lead further identification their roles functions metabolic

Language: Английский

Citations

6
Improving detection of potato virus X resistance genes Rx1 and Rx2 in tetraploid potato using optimized PCR and KASP markers DOI Creative Commons
Saiful Islam, Jiana Li, Jie Zheng

et al.

Horticultural Plant Journal, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Bulked Segregant RNA Sequencing (BSR-Seq) Combined with SNP Genotyping Towards Mapping and Characterization of a Purple Blotch Resistance Gene in Onion (Allium cepa L.) DOI

Jayashree Sahoo,

Rukmini Mishra, Raj Kumar Joshi

et al.

Plant Molecular Biology Reporter, Journal Year: 2024, Volume and Issue: unknown

Published: May 18, 2024

Language: Английский

Citations

1
Priority directions of onion breeding (Allium cepa L.) DOI Creative Commons
M. Marcheva, Т. М. Середин, I. M. Kaigorodova

et al.

VEGETABLE CROPS OF RUSSIA, Journal Year: 2024, Volume and Issue: 6, P. 30 - 43

Published: Nov. 30, 2024

Relevance . Onion ( Allium cepa L.) is a valuable and demanded vegetable crop, which occupies one of the leading places in terms sown areas gross yields among other crops genus L. popular folk evidence-based medicine, widely used for prevention treatment many diseases due to its rich biochemical composition useful properties. As part import substitution achieving food security country, scientists Federal State Budgetary Scientific Institution «Federal Vegetable Center» (FSBSI FSVC) are working intensively create new generation varieties hybrids. Methodology Various resources on topic were selected, publications scientific industry journals databases studied, review works onion selection (yield, species diversity shape color, maturity, shelf life, parameters, commercial seed productivity, resistance biotic abiotic stresses), as well properties humans use medicine was made. Results The analysis shows that wide distribution Russian Federation have such FSBSI FSVC, Myachkovskij 300, Cherni prince, Al'ba, Atas, Ampeks others. In recent years, created medium-maturing hybrid F 1 Drakon with brown dry scales maximum yield 391.0 c/ha, transferred variety trial winter Novator scales. Propagation hybrids both production cultivation household farms underway. Conclusion order conveyor production, it necessary different maturity groups, work improving marketability, productivity various stresses.

Language: Английский

Citations

0
Integrated Transcriptomic and Metabolomic Analysis of G. hirsutum and G. barbadense Responses to Verticillium Wilt Infection DOI Open Access

Baoguang Xing,

Pengtao Li,

Yanfang Li

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 28 - 28

Published: Dec. 24, 2024

Verticillium wilt (VW) caused by dahliae (Vd) is a devastating fungal cotton disease characterized high pathogenicity, widespread distribution, and frequent variation. It leads to significant losses in both the yield quality of cotton. Identifying key non-synonymous single nucleotide polymorphism (SNP) markers crucial genes associated with VW resistance Gossypium hirsutum barbadense, subsequently breeding new disease-resistant varieties, are essential for management. Here, we sequenced transcriptome metabolome roots TM-1 (G. hirsutum) Hai7124 barbadense) after 0, 1, 2 days V991 inoculation. Transcriptome analysis identified total 72,752 genes, 5814 differentially expressed (DEGs) determined through multiple group comparisons. KEGG enrichment revealed that pathways enriched DEGs obtained from longitudinal transverse comparisons contained glutathione metabolism pathway. Metabolome 995 metabolites, 22 accumulated metabolites (DAMs), which were correlated including metabolism, degradation valine, leucine, isoleucine, biosynthesis terpenoids, alkaloids, pyridine, piperidine. The conjoint transcriptomic metabolomic sequencing DAMs pathway, candidate gene GH_D11G2329 (glutathione S-transferase, GSTF8) potentially response infection was selected. These findings establish basis investigating mechanisms underlying plant’s VW.

Language: Английский

Citations

0