Nanomaterials for mRNA‐based therapeutics: Challenges and opportunities

Bioengineering & Translational Medicine, Journal Year: 2023, Volume and Issue: 8(3)

Published: Jan. 29, 2023

Abstract Messenger RNA (mRNA) holds great potential in developing immunotherapy, protein replacement, and genome editing. In general, mRNA does not have the risk of being incorporated into host need to enter nucleus for transfection, it can be expressed even nondividing cells. Therefore, mRNA‐based therapeutics provide a promising strategy clinical treatment. However, efficient safe delivery remains crucial constraint application therapeutics. Although stability tolerability enhanced by directly retouching structure, there is still an urgent improve mRNA. Recently, significant progress has been made nanobiotechnology, providing tools nanocarriers. Nano‐drug system used loading, protecting, releasing biological microenvironment stimulate translation develop effective intervention strategies. present review, we summarized concept emerging nanomaterials latest enhancing function mRNA, primarily focusing on role exosomes delivery. Moreover, outlined its applications so far. Finally, key obstacles nanocarriers are emphasized, strategies overcome these proposed. Collectively, nano‐design materials exert functions specific applications, new perception next‐generation nanomaterials, thus revolution technology.

Language: Английский

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Research Status and Prospect of Non-Viral Vectors Based on siRNA: A Review

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(4), P. 3375 - 3375

Published: Feb. 8, 2023

Gene therapy has attracted much attention because of its unique mechanism action, non-toxicity, and good tolerance, which can kill cancer cells without damaging healthy tissues. siRNA-based gene downregulate, enhance, or correct expression by introducing some nucleic acid into patient Routine treatment hemophilia requires frequent intravenous injections missing clotting protein. The high cost combined causes most patients to lack the best resources. siRNA potential lasting even curing diseases. Compared with traditional surgery chemotherapy, fewer side effects less damage normal cells. available therapies for degenerative diseases only alleviate symptoms patients, while drugs upregulate expression, modify epigenetic changes, stop disease. In addition, also plays an important role in cardiovascular diseases, gastrointestinal hepatitis B. However, free is easily degraded nuclease a short half-life blood. Research found that be delivered specific through appropriate vector selection design improve therapeutic effect. application viral vectors limited their immunogenicity low capacity, non-viral are widely used immunogenicity, production cost, safety. This paper reviews common recent years introduces advantages disadvantages, as well latest examples.

Language: Английский

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Expanding CAR-T cell immunotherapy horizons through microfluidics

Lab on a Chip, Journal Year: 2024, Volume and Issue: 24(5), P. 1088 - 1120

Published: Jan. 1, 2024

This review outlines Chimeric antigen receptor (CAR)-T cell manufacturing, highlights challenges, and explores successful microfluidic approaches related technologies to address them.

Language: Английский

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Advances in liposome-based delivery of RNA therapeutics for cancer treatment

Progress in molecular biology and translational science, Journal Year: 2024, Volume and Issue: unknown, P. 177 - 218

Published: Jan. 1, 2024

Language: Английский

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Anticancer and Antibacterial Properties of Curcumin-Loaded Mannosylated Solid Lipid Nanoparticles for the Treatment of Lung Diseases

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(4), P. 2175 - 2185

Published: March 13, 2024

Lung cancer and Mycobacterium avium complex infection are lung diseases associated with high incidence mortality rates. Most conventional anticancer drugs antibiotics have certain limitations, including drug resistance rates adverse effects. Herein, we aimed to synthesize mannose surface-modified solid lipid nanoparticles (SLNs) loaded curcumin (Man-CUR SLN) for the effective treatment of disease. The synthesized Man-CUR SLNs were analyzed using various instrumental techniques structural physicochemical characterization. Loading into improved encapsulation efficiency release capacity, as demonstrated by high-performance liquid chromatography analysis. Furthermore, characterized effect A549 cell line. Cells treated SLN exhibited an increased cellular uptake cytotoxicity. Moreover, free CUR could more effectively reduce migration than SLNs. Similarly, elicited a stronger apoptosis-inducing that SLNs, reverse transcription-quantitative PCR Finally, examined antibacterial effects against intracellulare (M.i.) M.i.-infected macrophages, revealing exerted strongest effect. Collectively, these findings indicate mannose-receptor-targeted delivery be in treating diseases. Accordingly, this system can used target variety cancers immune cells.

Language: Английский

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Advancing Therapeutic Strategies with Polymeric Drug Conjugates for Nucleic Acid Delivery and Treatment

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 25 - 52

Published: Jan. 1, 2025

Abstract: The effective clinical translation of messenger RNA (mRNA), small interfering (siRNA), and microRNA (miRNA) for therapeutic purposes hinges on the development efficient delivery systems. Key challenges include their susceptibility to degradation, limited cellular uptake, inefficient intracellular release. Polymeric drug conjugates (PDCs) offer a promising solution, combining benefits polymeric carriers agents targeted treatment. This comprehensive review explores nucleic acid therapeutics, focusing conjugates. It investigates how these address obstacles, enhance systemic circulation, reduce immunogenicity, provide controlled release, improving safety profiles. delves into conjugation strategies, preparation methods, various classes PDCs, as well strategic design, highlighting role in delivery. Applications PDCs treating diseases such cancer, immune disorders, fibrosis are also discussed. Despite significant advancements, adoption persist. concludes with insights future directions this transformative technology, underscoring potential advance acid-based therapies combat infectious significantly. Keywords: polymer conjugates,

Language: Английский

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Integrating RNA Interference and Nanotechnology: A Transformative Approach in Plant Protection

Plants, Journal Year: 2025, Volume and Issue: 14(6), P. 977 - 977

Published: March 20, 2025

RNA interference (RNAi) has emerged as a potent mechanism for combating pathogenic fungi and oomycetes over the past decades. It offers promising gene-silencing approach by targeting crucial genes involved in diseases caused economically scientifically significant fungal pathogens, such Botrytis cinerea Fusarium species. Simultaneously, nano-agro-products have gained attention alternatives to traditional fungicides plant protection strategies. However, instability of naked molecules outside cellular environment presents challenge, they degrade rapidly, limiting their efficacy prolonged disease control. Concerns regarding toxicity protective nanoparticles non-target organisms also arisen. Integrating RNAi with nano-agro-products, particularly nanocarriers, form RNA-nano complexes demonstrated potential, providing enhanced stability, reduced toxicity, extended This review explores mechanisms complexes-mediated protection, addressing stability nano-toxicity issues while examining prospects complex research pathogen management.

Language: Английский

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Development of mRNA Vaccines/Therapeutics and Their Delivery System

Molecules and Cells, Journal Year: 2023, Volume and Issue: 46(1), P. 41 - 47

Published: Jan. 1, 2023

The rapid development of mRNA vaccines has contributed to the management current coronavirus disease 2019 (COVID-19) pandemic, suggesting that this technology may be used manage future outbreaks infectious diseases. Because antigens targeted by can easily altered simply changing sequence present in coding region structures, it is more appropriate develop vaccines, especially during rapidly developing In addition allowing development, have great potential inducing successful antigen-specific immunity expressing target cells and simultaneously triggering immune responses. Indeed, two COVID-19 approved U.S. Food Drug Administration shown significant efficacy preventing infections. ability mRNAs produce proteins are defective specific diseases enabled options treat intractable Clinical applications vaccines/therapeutics require strategies safely deliver RNA molecules into cells. review summarizes knowledge about vaccines/ therapeutics, their clinical applications, delivery strategies.

Language: Английский

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Lipid-based colloidal nanoparticles for applications in targeted vaccine delivery

Nanoscale Advances, Journal Year: 2023, Volume and Issue: 5(7), P. 1853 - 1869

Published: Jan. 1, 2023

This review article demonstrated nucleic acid-based therapeutics and lipid nanoparticle type as a carrier of acid for further applications LNPs flexible carriers in immuno-therapeutics anti-cancer reagents.

Language: Английский

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Novel Vectors and Administrations for mRNA Delivery

Small, Journal Year: 2023, Volume and Issue: 19(46)

Published: July 20, 2023

Abstract mRNA therapy has shown great potential in infectious disease vaccines, cancer immunotherapy, protein replacement therapy, gene editing, and other fields due to its central role all life processes. However, is challenging pass through the cell membrane significant negative charges degradation from RNase, so key efficient packaging delivery of it with appropriate vectors. Presently researchers have developed various vectors such as viruses liposomes, but these conventional are now difficult meet growing requirement like safety, efficiency, targeting, many novel unique advantages emerged recently. This review mainly introduces two categories vectors: biomacromolecules inorganic nanoparticles, well methods control administration based on controlled‐release non‐invasive administration. These strategies high biocompatibility, versatility, intelligence, targeting. paper analyzes challenges faced by field depth, discusses how use characteristics administrations solve problems.

Language: Английский

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