One-Step Immunoassay for the Detection of Influenza Virus Based on Screened Nucleoprotein (NP)-like Mimotopes from Fv-Antibody Library DOI
Tae‐Hun Kim, Jaeyong Jung,

Jeong Soo Sung

et al.

BioChip Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 29, 2024

Language: Английский

Screening of deoxyribonuclease I inhibitors from autodisplayed Fv-antibody library DOI
Hyung Eun Bae, Jaeyong Jung,

Jeong Soo Sung

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140770 - 140770

Published: Feb. 1, 2025

Language: Английский

Citations

0
Oxygen-Terminated Silicon Carbide (SiC) Particle-Coated Electrodes for Highly Sensitive Electrochemical Immunoassays DOI
Eun Young Kim, Seong‐Min Jeong, Ga-Yeon Lee

et al.

BioChip Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Language: Английский

Citations

0
Transmembrane protease serine 2 (TMPRSS2) inhibitors screened from an Fv-antibody library for preventing SARS-CoV-2 infection DOI Creative Commons
Jaeyong Jung,

Jeong Soo Sung,

Soonil Kwon

et al.

RSC Medicinal Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

TMPRSS2 inhibitors used to prevent SARS-CoV-2 infection, which were screened from an Fv-antibody library.

Language: Английский

Citations

0
Preventing SARS-CoV-2 infection using Fv-antibodies targeting the proprotein convertase (PPC) cleavage site DOI
Jaeyong Jung,

Jeong Soo Sung,

Soonil Kwon

et al.

RSC Medicinal Chemistry, Journal Year: 2024, Volume and Issue: 15(11), P. 3704 - 3710

Published: Jan. 1, 2024

Fv-antibodies targeting the proprotein convertase (PPC) region of SARS-CoV-2 spike protein (SP) were screened from an Fv-antibody library to inhibit infection. Two selected expressed as soluble recombinant proteins, and their binding affinities assessed using a surface plasmon resonance biosensor. The regions these corresponded cleavage sites furin (S1/S2) transmembrane serine protease 2 (TMPRSS2, S2'). neutralizing activities two demonstrated cell-based infection assay with pseudo-viruses carrying SP four different variants: wild-type (D614), delta (B.1.617.2), omicron (BA.2), (BA.4/5).

Language: Английский

Citations

1
Screened Fv-Antibodies against the Angiotensin-Converting Enzyme 2 (ACE2) Receptor Neutralizing the Infection of SARS-CoV-2 DOI
Jaeyong Jung, Soonil Kwon,

Jeong Soo Sung

et al.

ACS Pharmacology & Translational Science, Journal Year: 2024, Volume and Issue: 7(12), P. 3914 - 3920

Published: Nov. 19, 2024

For the prevention of SARS-CoV-2 infection, four Fv-antibodies with binding affinity for ACE2 receptor were screened from an Fv-antibody library. The expressed as soluble proteins and estimated to have a high affinity, comparable that between receptor. interaction was analyzed using docking simulation, significant attributed homology in amino acid sequence neutralizing activities demonstrated cell-based infection assay based on pseudo-virus types variant spike (Wild-type D614, Delta B.1.617.2, Omicron BA.2, BA.4/5).

Language: Английский

Citations

1
Plasma Deposition of Parylene-like Films with Chemical Functional Groups for Immunoassays DOI
Zhiquan Song, Jaeyong Jung, Tae‐Hun Kim

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(46), P. 63255 - 63267

Published: Nov. 6, 2024

Parylene-like films obtained via the plasma decomposition of parylene precursors with functional groups (amino and formyl) are proposed as an alternative to those thermal method. To analyze chemical after deposition, a surface analysis using two different deposition methods was performed Fourier transform infrared (FT-IR) X-ray photoelectron spectroscopy (XPS). The FT-IR revealed that featured peaks were maintained in parylene-like XPS parylene-AM parylene-H deposition. energy estimated by contact angle measurements. plasma-deposited then employed for protein immobilization horseradish peroxidase (44 kDa) green fluorescent (25 model proteins. exhibited higher efficiencies than did same Finally, competitive immunoassay immobilizing Fv-antibodies on covalent bonding. Using heat-deactivated SARS-CoV-2 real sample, limit detection at feasible level medical diagnosis COVID-19 achieved based immobilized films.

Language: Английский

Citations

0
One-step Ultra-Rapid Immunoassay of Calcitonin Gene-Related Peptide for Migraine Diagnosis DOI

Jeong Soo Sung,

Jaeyong Jung, Soon Kil Kwon

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 270, P. 116980 - 116980

Published: Nov. 23, 2024

Language: Английский

Citations

0
One-Step Immunoassay for the Detection of Influenza Virus Based on Screened Nucleoprotein (NP)-like Mimotopes from Fv-Antibody Library DOI
Tae‐Hun Kim, Jaeyong Jung,

Jeong Soo Sung

et al.

BioChip Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 29, 2024

Language: Английский

Citations

0