New Species of Diaporthales (Ascomycota) from Diseased Leaves in Fujian Province, China DOI Creative Commons
Xiayu Guan, Taichang Mu, Nemat O. Keyhani

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 11(1), P. 8 - 8

Published: Dec. 26, 2024

Fungal biota represents important constituents of phyllosphere microorganisms. It is taxonomically highly diverse and influences plant physiology, metabolism health. Members the order Diaporthales are distributed worldwide include devastating pathogens as well endophytes saprophytes. However, many species remain uncharacterized, with studies examining their diversity needed. Here, we report on identification several diaporthalean taxa samples collected from diseased leaves Cinnamomum camphora (Lauraceae), Castanopsis fordii (Fagaceae) Schima superba (Theaceae) in Fujian province, China. Based morphological features coupled to multigene phylogenetic analyses internal transcribed spacer (ITS) region, large subunit nuclear ribosomal RNA (LSU), partial beta-tubulin (tub2), histone H3 (his3), DNA-directed polymerase II (rpb2), translation elongation factor 1-α (tef1) calmodulin (cal) genes, three new introduced, namely, Diaporthe wuyishanensis, Gnomoniopsis wuyishanensis Paratubakia schimae. This study contributes our understanding biodiversity fungi that inhabitants trees native Asia.

Language: Английский

Three New Records of Pathogens Causing Stem Blight on Vaccinium corymbosum in China DOI Creative Commons
Yueyan Zhou,

Linna Wu,

K. J. Ren

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(5), P. 647 - 647

Published: Feb. 20, 2025

Stem blight is a significant disease affecting blueberries worldwide, caused by various pathogens. This study investigated stem in Ji’an, Jilin Province, China. Fungi isolated from diseased stems were identified as Colletotrichum temperatum, Curvularia austriaca, and Diaporthe unshiuensis based on morphological characters multi-locus phylogenetic analyses using the internal transcribed spacer (ITS) region, glyceraldehyde 3-phosphate dehydrogenase (gapdh), chitin synthase (chs), actin (act), β-tubulin (tub2), translation elongation factor 1-alpha (tef1-α), calmodulin (cal), histone 3 (his3) regions. Pathogenicity test was conducted detached green blueberry shoots, all shoots inoculated mycelium plugs presented necrotic lesions with dark brown margins, while control (PDA plugs) group did not show any symptoms. Koch’s postulates confirmed re-isolating pathogen The provides three new host–pathogen records of fungi associated blight.

Language: Английский

Citations

1
Novel genus and species of Diaporthostomataceae (Diaporthales) in China DOI Creative Commons
Ning Jiang, Han Xue, Yong Li

et al.

IMA Fungus, Journal Year: 2025, Volume and Issue: 16

Published: March 7, 2025

Diaporthales is a significant fungal order comprising species that predominantly inhabit plant tissues, being pathogens, endophytes, and saprobes. Recent studies have uncovered extensive diversity across various hosts, utilizing both morphological characteristics molecular phylogenetic analyses. In this study, samples of leaf spots branch cankers were collected from China, isolations established. Species identification was conducted using approach based on combined sequence data the internal transcribed spacer (ITS) region, large subunit ribosomal DNA (LSU), DNA-directed RNA polymerase II second largest (rpb2), translation elongation factor 1-alpha (tef1) genes, together with observations. As result, novel genus Tiania proposed, three newly described species: T.chinensis, T.lithocarpicola, T.quercicola. These are validated by pairwise homoplasy index (PHI) analysis, ensuring robust support for their distinction. This study explores rare family Diaporthostomataceae, providing first descriptions anamorphic forms. By offering detailed data, research lays foundation future taxonomic systematic Diaporthales.

Language: Английский

Citations

1
Mango Microcosm: Unveiling the species diversity and pathogenic potential of fungi isolated from mango (Mangifera indica) in northern Thailand DOI

Alireza Armand,

Maryam Fallahi, Sahar Absalan

et al.

Fungal Biology, Journal Year: 2025, Volume and Issue: 129(3), P. 101552 - 101552

Published: Feb. 22, 2025

Language: Английский

Citations

0
The six whole mitochondrial genomes for the Diaporthe species: features, evolution and phylogeny DOI Creative Commons

Shunpei Xie,

Xuyang Ma,

Haiyan Wu

et al.

IMA Fungus, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 28, 2025

In this study, the complete mitogenomes of three Diaporthe species (Diaportheeres ZM79-3, D.phaseolorum ZM33-4 and sp. ZM41-5) were sequenced, assembled compared with other previously sequenced (D.caulivora VNIIKR SE Dcaul3, D.longicolla MSPL 10-6 D.sojae Dps12). The six found to be circular DNA molecules, lengths ranging from 53,646 bp 108,865 bp. mainly comprised same set 15 core protein-coding genes (PCGs), two rRNAs, a certain number tRNAs unidentified open reading frames (ORFs). PCG length, AT skew GC showed large variability among PCGs in mitogenomes. nad1 gene had least K2P genetic distance 13 Diaporthales species, indicating that was highly conserved. Ka/Ks values for all < 1, suggesting these subject purifying selection. Comparative mitogenome analysis introns contributed most size variation Frequent intron loss/gain events detected have occurred cox1 during evolution Although undergone large-scale rearrangements, identical arrangements. Phylogenetic based on combined mitochondrial datasets formed well-supported topologies. To our knowledge, study is first report ZM41-5, as well comparison species. Our findings will further promote investigations genetics, phylogeny

Language: Английский

Citations

0
Challenges and update on fungal endophytes: classification, definition, diversity, ecology, evolution and functions DOI Creative Commons
Chun-Fang Liao, Mingkwan Doilom,

Rajesh Jeewon

et al.

Fungal Diversity, Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

Language: Английский

Citations

0
Micro-fungi in southern China and northern Thailand: emphasis on medicinal plants DOI
Ya-Ru Sun, Kevin D. Hyde, Ning-Guo Liu

et al.

Fungal Diversity, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

Language: Английский

Citations

0
Unveiling Sordariomycetes taxa associated with woody litter in Northern Thailand DOI Creative Commons
Chayanard Phukhamsakda, Kevin D. Hyde, Milan C. Samarakoon

et al.

MycoKeys, Journal Year: 2025, Volume and Issue: 115, P. 155 - 185

Published: March 17, 2025

Sordariomycetes species are abundant in woody litter samples. In this study, we introduce two novel species, Diaporthe thailandica (Diaporthaceae) and Occultitheca chiangraiensis (Xylariaceae), from materials. We also describe a new host record of D. tulliensis geographical for melonis. All collections were identified based on morphology phylogenetic analyses combined datasets. The morphologies the taxa fit generic concepts , respectively. formed sister clade with raonikayaporum but differs sizes conidiomata, conidiogenous cells, beta conidia. by absence gamma taxon O. rosae having smaller ascomata thicker mucilaginous sheath. provide synopsis details their morphology, host, country. These findings valuable insights into diversity ecological roles Sordariomycetes, emphasising need continued exploration fungal biodiversity various environments.

Language: Английский

Citations

0
Deciphering the evolutionary and taxonomic complexity of Diaporthales (Sordariomycetes, Ascomycota) through integrated phylogenomic and divergence time estimation DOI Creative Commons
Zhaoxue Zhang,

Yu-Xin Shang,

Qiyun Liu

et al.

Fungal Diversity, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

Language: Английский

Citations

0
Exploring Fungicide Sensitivity in Soybean Stem Blight Pathogen Diaporthe longicolla, Emphasizing Genetic Variability Impact on Response to SDHI Fungicides Fluopyram and Pydiflumetofen DOI Creative Commons
Shanshan Chen,

Zhanyun Liu,

Zhenyun Chang

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(4), P. 292 - 292

Published: April 8, 2025

Diaporthe species are critical plant pathogens that contribute to a disease complex responsible for substantial yield losses in soybean production worldwide. However, reports on the primary causing stem blight and their sensitivity various fungicides scarce China. In this study, total of 46 D. longicolla strains were isolated identified from diseased stems rots collected 14 regions Heilongjiang province 2021 2022. Among eight examined, fludioxonil, mefentrifluconazole, tebuconazole, azoxystrobin demonstrated effective inhibition longicolla, with EC50 values < 0.3 µg/mL. Interestingly, two succinate dehydrogenase inhibitors (SDHIs), pydiflumetofen fluopyram, 5.47 µg/mL over 100 µg/mL, respectively. molecular dynamics simulations, exhibited smaller RMSD, while fluopyram had higher binding free energy Sdh proteins compared pydiflumetofen. This difference may activity longicolla. Further analysis electrostatic potential structural conformations pocket revealed formed more hydrophobic interactions SdhC SdhD was positioned closer subunit. A mixture fludioxonil mefentrifluconazole at ratio 1:5, as well synergistic effects. These findings several could be utilized control blight, affinity subunit impacts

Language: Английский

Citations

0
Species of Diaporthe (Diaporthaceae, Diaporthales) associated with Alnus nepalensis leaf spot and branch canker diseases in Xizang, China DOI Creative Commons

Jie‐Ting Li,

Li Yi,

Jiangrong Li

et al.

MycoKeys, Journal Year: 2025, Volume and Issue: 116, P. 185 - 204

Published: April 23, 2025

Alnusnepalensis is an important tree species in the Himalayas with significant ecological and economic roles. During disease surveys Xizang, China, we observed leaf spot branch canker symptoms on this tree. Fungal isolates associated these diseases were collected identified based morphological characteristics phylogenetic analysis of ITS, cal, his3, tef1, tub2 sequences. As a result, Diaporthealnicola sp. nov. D.amygdali from spots, while D.linzhiensis was to be cankered branches. This study identifies pathogenic alder trees, providing foundation for future management forest health research.

Language: Английский

Citations

0