CRISPR/Cas genome editing for cotton precision breeding: mechanisms, advances, and prospects

Journal of Cotton Research, Journal Year: 2025, Volume and Issue: 8(1)

Published: Feb. 3, 2025

Abstract Cotton ( Gossypium hirsutum L.) is one of the most important global crops that supports textile industry and provides a living for millions farmers. The constantly increasing demand needs significant rise in cotton production. Genome editing technology, specifically with clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) tools, has opened new possibilities trait development cotton. It allows precise efficient manipulation within genome when compared other genetic engineering tools. Current developments CRISPR/Cas including prime editing, base multiplexing have expanded scope traits breeding can be targeted. been employed to generate effectively CRISPRized plants enhanced agronomic traits, fiber yield quality, oil improvement, stress resistance, nutrition. Here we summarized various target genes which successfully altered However, some challenges remain, tetraploid having redundant gene sets homologs making editing. To ensure specificity avoiding off-target effects, need optimize parameters such as site, guide RNA design, choosing right Cas variants. We outline future prospects breeding, suggesting areas further research innovation. A combination speed might useful fastening potentials create customized cultivars meet higher demands agriculture industry.

Language: Английский

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Decoding Phytohormone Signaling in Plant Stress Physiology: Insights, Challenges, and Future Directions

Environmental and Experimental Botany, Journal Year: 2025, Volume and Issue: unknown, P. 106099 - 106099

Published: Feb. 1, 2025

Language: Английский

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Genome editing for food, nutrition, and health

The Nucleus, Journal Year: 2024, Volume and Issue: 67(1), P. 1 - 4

Published: April 1, 2024

A new era of genetics dawned in the 2010s with discovery revolutionary CRISPR/Cas technology, which enables targeting and editing specific genes or genomic loci high precision [4].Since then, CRISPR-Cas has become a household name, as it quite literally democratized genome across globe due to its versatility, flexibility, efficiency, relative ease adoption diverse biological systems.Conventional CRISPR-Cas9 advanced tools like base editors prime have principal for disrupting gene functions, replacing alleles, creating precise insertions deletions, conducting large-scale chromosome engineering [11].However, there are numerous challenges that must be addressed fully realize potential this technology.Base Editing (BE), Prime (PE) Homology-Directed Repair (HDR) offer capability allele swapping.However, efficiency PE HDR higher eukaryotes, particularly plants, is currently low requires further enhancement enable routine application crop improvement [17].The delivery CRISPR reagents cell types their encapsulation within cargo vehicles pose significant challenges.Furthermore, regeneration edited plants remains problematic many recalcitrant crops.Addressing polyploid crops necessitates dedicated attention.

Language: Английский

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Abiotic Stress in Cotton: Insights into Plant Responses and Biotechnological Solutions

Agriculture, Journal Year: 2024, Volume and Issue: 14(9), P. 1638 - 1638

Published: Sept. 19, 2024

Climate change has rapidly increased incidences of frequent extreme abiotic stresses, such as heat, drought, salinity, and waterlogging. Each these stressors negatively affects the cotton crop (Gossypium spp.) results in significant yield decreases. Every stressful event causes specific changes metabolism physiology plants, which are linked to complex molecular alterations. Understanding mechanisms that regulate a plant’s response stress is essential developing stress-resistant varieties can withstand various factors. Gene expressions multiple stresses have been studied mapped. These genes include ion transporters heat shock proteins, vital allowing adaptive responses. approaches showed ability employ advanced genome sequencing multi-omics techniques identify dynamic gene expression patterns elucidate intricate regulatory networks. Using genetic variation combination with techniques, it would be possible generate stress-resilient enable sustainable output face stresses. Here, we reviewed effects major on heavy metals, We also examine vast network genes, stress-sensitive signaling pathways help tolerate stress.

Language: Английский

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Revolutionizing Cotton Cultivation: A Comprehensive Review of Genome Editing Technologies and Their Impact on Breeding and Production

Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 742, P. 151084 - 151084

Published: Nov. 27, 2024

Language: Английский

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Development of virus-resistant horticultural crops through CRISPR/Cas mediated genome editing: applications and future prospects

The Nucleus, Journal Year: 2024, Volume and Issue: unknown

Published: June 25, 2024

Language: Английский

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