
Technology in Agronomy, Journal Year: 2024, Volume and Issue: 4(1), P. 0 - 0
Published: Jan. 1, 2024
Language: Английский
Technology in Agronomy, Journal Year: 2024, Volume and Issue: 4(1), P. 0 - 0
Published: Jan. 1, 2024
Language: Английский
Molecular Genetics and Genomics, Journal Year: 2025, Volume and Issue: 300(1)
Published: Feb. 21, 2025
Language: Английский
Citations
0The Nucleus, Journal Year: 2024, Volume and Issue: 67(1), P. 1 - 4
Published: April 1, 2024
A new era of genetics dawned in the 2010s with discovery revolutionary CRISPR/Cas technology, which enables targeting and editing specific genes or genomic loci high precision [4].Since then, CRISPR-Cas has become a household name, as it quite literally democratized genome across globe due to its versatility, flexibility, efficiency, relative ease adoption diverse biological systems.Conventional CRISPR-Cas9 advanced tools like base editors prime have principal for disrupting gene functions, replacing alleles, creating precise insertions deletions, conducting large-scale chromosome engineering [11].However, there are numerous challenges that must be addressed fully realize potential this technology.Base Editing (BE), Prime (PE) Homology-Directed Repair (HDR) offer capability allele swapping.However, efficiency PE HDR higher eukaryotes, particularly plants, is currently low requires further enhancement enable routine application crop improvement [17].The delivery CRISPR reagents cell types their encapsulation within cargo vehicles pose significant challenges.Furthermore, regeneration edited plants remains problematic many recalcitrant crops.Addressing polyploid crops necessitates dedicated attention.
Language: Английский
Citations
3The Nucleus, Journal Year: 2024, Volume and Issue: unknown
Published: June 25, 2024
Language: Английский
Citations
0Technology in Agronomy, Journal Year: 2024, Volume and Issue: 4(1), P. 0 - 0
Published: Jan. 1, 2024
Language: Английский
Citations
0