iJAZ-based approach to engineer lepidopteran pest resistance in multiple crop species

Nature Plants, Journal Year: 2024, Volume and Issue: 10(5), P. 771 - 784

Published: April 29, 2024

Language: Английский

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Differential detoxification enzyme profiles in C-corn strain and R-rice strain of Spodoptera frugiperda by comparative genomic analysis: insights into host adaptation

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: Jan. 6, 2025

The fall armyworm (FAW) Spodoptera frugiperda, a highly invasive, polyphagous pest, poses global agricultural threat. It has two strains, the C-corn and R-rice each with distinct host preferences. This study compares detoxification enzyme gene families across these strains related species to explore their adaptation diverse plant metabolites. A total of 1,995 detoxification-related genes, including cytochrome P450 monooxygenases (CYPs), carboxylesterases (COEs), glutathione S-transferases (GSTs), UDP-glucuronosyltransferases (UGTs), ATP-binding cassette transporters (ABCs), were identified genus Spodoptera, S. littoralis, litura, picta, exigua, both FAW strains. higher abundance phase I enzymes (CYPs COEs) GSTs was observed in species, while exhibited fewer notable differences copy numbers between C R Analyses at subfamily level revealed significant variation distribution expression, particularly within II enzymes. Expansions CYP6AE detected strain, contractions GST-ε, CYP9A, CYP4M, UGT33B, UGT33F occurred In contrast, no substantial III ABC Functional predictions protein interaction networks suggest broader expansion metabolism-related genes strain compared strain. These findings emphasize pivotal role adaptation, providing molecular insights into FAW's capacity for range expansion, which are crucial devising targeted sustainable pest management strategies. differential expression different frugiperda.

Language: Английский

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Geographic Monitoring of Insecticide Resistance Mutations in Native and Invasive Populations of the Fall Armyworm

Insects, Journal Year: 2021, Volume and Issue: 12(5), P. 468 - 468

Published: May 18, 2021

Field evolved resistance to insecticides is one of the main challenges in pest control. The fall armyworm (FAW) a lepidopteran species causing severe crop losses, especially corn. While native Americas, presence FAW was confirmed West Africa 2016. Since then, has been detected over 70 countries covering sub-Saharan Africa, Middle East, North South Asia, Southeast and Oceania. In this study, we tested whether invasion accompanied by spread mutations from invasive areas. We observed that Bt at ABCC2 genes were only populations where initially reported. Invasive found have higher gene numbers cytochrome P450 than proportion multiple acetylcholinesterase genes, supporting strong selective pressure for against synthetic insecticides. This result explains susceptibility various Chinese populations. These results highlight necessity regular standardized monitoring insecticide using both genomic approaches bioassay experiments.

Language: Английский

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Comparison of Gut Bacterial Communities of Fall Armyworm (Spodoptera frugiperda) Reared on Different Host Plants

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(20), P. 11266 - 11266

Published: Oct. 19, 2021

Spodoptera frugiperda is a highly polyphagous and invasive agricultural pest that can harm more than 300 plants cause huge economic losses to crops. Symbiotic bacteria play an important role in the host biology ecology of herbivores, have wide range effects on growth adaptation. In this study, high-throughput sequencing technology was used investigate different hosts (corn, wild oat, oilseed rape, pepper, artificial diet) gut microbial community structure diversity. Corn one most favored S. frugiperda. We compared microbiota corn with without seed coating agent. The results showed Firmicutes Bacteroidetes dominated community. abundance rape highest, diversity oat lowest, agent significantly higher such PCoA analysis there were significant differences among hosts. PICRUSt functional prediction categories related metabolic cellular processes. affected not only by species, but also treatments, which played It deepen our understanding symbiotic relationships between organisms microorganisms. study adaptability insects contributes development effective environmentally friendly management strategies.

Language: Английский

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Soil-derived bacteria endow Camellia weevil with more ability to resist plant chemical defense

Microbiome, Journal Year: 2022, Volume and Issue: 10(1)

Published: June 25, 2022

Abstract Background Herbivorous insects acquire their gut microbiota from diverse sources, and these microorganisms play significant roles in insect hosts’ tolerance to plant secondary defensive compounds. Camellia weevil ( Curculio chinensis ) (CW) is an obligate seed parasite of oleifera plants. Our previous study linked the CW’s microbiome tea saponin (TS) C. seeds. However, source microbiomes, key bacteria involved TS tolerance, degradation functions remain unresolved. Results indicated that CW was more affected by soil than fruits. The soil-derived Acinetobacter served as core bacterial genus, sp. putatively regarded responsible for saponin-degradation guts. Subsequent experiments using fluorescently labeled cultures verified isolate AS23 can migrate into larval guts, ultimately endow its host with ability degrade saponin, thereby allowing subsist a pest within fruits resisting higher concentration chemical. Conclusions systematic studies sources microorganisms, screening taxa metabolite degradation, investigation toxicity mitigation provide clarified evidence intestinal mediate herbivorous against toxins.

Language: Английский

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Global genomic signature reveals the evolution of fall armyworm in the Eastern hemisphere

Molecular Ecology, Journal Year: 2023, Volume and Issue: 32(20), P. 5463 - 5478

Published: Aug. 28, 2023

The major plant pest fall armyworm (FAW), Spodoptera frugiperda, is native to the Americas and has colonized Africa Asia within Eastern hemisphere since 2016, causing severe damage multiple agricultural crop species. However, genetic origin of these invasive populations requires more in-depth exploration. We analysed variation across genomes 280 FAW individuals from both Americas. global range-wide structure shows that in America experienced deep differentiation, largely consistent with Z-chromosomal Tpi haplotypes commonly used differentiate 'corn-strain' 'rice-strain' populations. are different American ones have a relatively homogeneous population structure, common recent spreading Asia. Our analyses suggest north- central most likely sources invasion into hemisphere. Furthermore, evidence based on genomic, transcriptomic mitochondrial haplotype network indicates an earlier, independent introduction Africa, subsequent migration population.

Language: Английский

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Molecular interaction network of plant-herbivorous insects

Advanced Agrochem, Journal Year: 2023, Volume and Issue: 3(1), P. 74 - 82

Published: Sept. 2, 2023

The interactions between plants and herbivorous insects are complex involve multiple factors, driving species formation leading to the beginning of co-evolution diversification plant insect molecules. Various molecular processes regulate insects. Here, we discuss patterns perception feeding through activation early signaling components, crosstalk defense network composed hormones, various adaptive changes in responses defenses. Both defenses counter-defenses adaptation each other. Molecular models plant-herbivorous can more intuitively help us understand co-evolutionary arms race These results will provide detailed evidence elucidate enrich interaction

Language: Английский

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Chromosome-level genome of black cutworm provides novel insights into polyphagy and seasonal migration in insects

BMC Biology, Journal Year: 2023, Volume and Issue: 21(1)

Published: Jan. 5, 2023

Abstract Background The black cutworm, Agrotis ipsilon , is a serious global underground pest. Its distinct phenotypic traits, especially its polyphagy and ability to migrate long distances, contribute widening distribution increasing difficulty of control. However, knowledge about these traits still limited. Results We generated high-quality chromosome-level assembly A. using PacBio Hi-C technology with contig N50 length ~ 6.7 Mb. Comparative genomic transcriptomic analyses showed that detoxification-associated gene families were highly expanded induced after insects fed on specific host plants. Knockout genes encoded two ABC transporters CRISPR/Cas9 significantly reduced larval growth rate, consistent their contribution adaptation. A comparative analysis between tethered-flight moths migrating expression changes in the circadian rhythm AiCry2 involved sensing photoperiod variations may receipt magnetic fields accompanied by MagR regulate juvenile hormone pathway energy metabolism, all migration processes. Conclusions This study provides valuable resources for elucidating mechanisms moth developing innovative control strategies.

Language: Английский

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Omics: A new, promising technologies for boosting crop yield and stress resilience in African agriculture

Plant Stress, Journal Year: 2024, Volume and Issue: 11, P. 100366 - 100366

Published: Jan. 17, 2024

African agricultural systems are now in crucial need to assure food for the overgrowing population. To meet this challenges, we better way identify and modify key proteins genes involved yield potential, nutrient value, stress tolerance crops like maize, sorghum, soybeans, cassava, rice, etc. The deep understanding of metabolomics, transcriptomics, proteomics, genomics has proven be a useful tool increasing crop resilience Africa. However, there is very little information available regarding recent use omics technology increase yields This review provided advances plants molecular profiling techniques enhance our breeding strategies grown study will plant technologies inform strategies.

Language: Английский

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Knockout of the odorant receptor co-receptor, orco, impairs feeding, mating and egg-laying behavior in the fall armyworm Spodoptera frugiperda

Insect Biochemistry and Molecular Biology, Journal Year: 2022, Volume and Issue: 152, P. 103889 - 103889

Published: Dec. 7, 2022

Language: Английский

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