Double Peptide-Functionalized Carboxymethyl Chitosan-Coated Liposomes Loaded with Dexamethasone as a Potential Strategy for Active Targeting Drug Delivery

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 922 - 922

Published: Jan. 22, 2025

Liposomes are intensively used as nanocarriers for biology, biochemistry, medicine, and in the cosmetics industry their non-toxic biocompatible nature makes these vesicles attractive systems biomedical applications. Moreover, conjugation of specific ligands to liposomes increases cellular uptake therapeutic efficiency. Considering aspects, aim present study was obtain new formulations cationic coated with dual-peptide functionalized carboxymethyl chitosan (CMCS) treatment inner ear diseases. In order achieve efficient active targeting ensuring a high efficacy treatment, CMCS Tet1 peptide, target cells, TAT ensure penetration. Furthermore, dexamethasone phosphate loaded model drug inflammation. The infrared spectroscopy confirmed functionalization two peptides. mean diameter uncovered varied between 167 198 nm whereas CMCS-coated ranged from 179 202 nm. TEM analysis showed spherical shape unilamellar structure liposomes. release efficiency after 24 h uncoated 37 40% it appeared that modulated this release. obtained results demonstrated hemocompatible since, tested concentration 100 µg/mL, liposome suspension had lysis erythrocytes lower than 2.5% 180 min incubation. addition, peptide-functionalized induced non-significant effect on viability normal V79-4 cells 48 h, at highest doses. Values 71.31% were recorded (CLCP-1), 77.28% (CLCP-2) 74.36% (CLCP-3), correlated cytotoxic effects 28.69%, 22.72%, 25.64%.

Language: Английский

---

Innovative Approaches to Enhancing the Biomedical Properties of Liposomes

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(12), P. 1525 - 1525

Published: Nov. 27, 2024

Liposomes represent a promising class of drug delivery systems that enhance the therapeutic efficacy and safety various pharmaceutical agents. Also, they offer numerous advantages compared to traditional methods, including targeted specific sites, controlled release, fewer side effects. This review meticulously examines methodologies employed in preparation characterization liposomal formulations. With rising incidence adverse reactions, there is pressing need for innovative strategies prioritize selectivity, specificity, safety. Nanomedicine promises revolutionize diagnostics treatments, addressing current limitations improving disease management, cancer, which remains major global health challenge. paper aims conduct comprehensive study on interest biomedical research regarding nanotechnology its implications further applications.

Language: Английский

---

Using Immunoliposomes as Carriers to Enhance the Therapeutic Effectiveness of Macamide N-3-Methoxybenzyl-Linoleamide

Neurology International, Journal Year: 2025, Volume and Issue: 17(3), P. 38 - 38

Published: March 3, 2025

Background/Objectives: Epilepsy is one of the most common chronic neurological disorders, characterized by alterations in neuronal electrical activity that result recurrent seizures and involuntary body movements. Anticonvulsants are primary treatment for this condition, helping patients improve their quality life. However, development new drugs with fewer side effects greater economic accessibility remains a key focus nanomedicine. Macamides, secondary metabolites derived from Maca (Lepidium meyenii), represent promising class novel diverse therapeutic applications, particularly disorders. Methods: In study, we optimized potential macamide N-3-methoxybenzyl-linoleamide (3-MBL) as an anticonvulsant agent through its encapsulation PEGylated liposomes conjugated OX26 F(ab′)2 fragments. Results: These immunoliposomes exhibited size 120.52 ± 9.46 nm zeta −8.57 0.80 mV. Furthermore, vivo tests using pilocarpine-induced status epilepticus model revealed provided efficacy against epileptic compared to free form at same dose. Notably, observed effect was comparable carbamazepine, traditional FDA-approved antiepileptic drug. Conclusions: This pioneering work employs liposomal nanocarriers deliver macamides brain, aiming set standard use modified epilepsy treatment.

Language: Английский

---

Choline metabolism in ischemic stroke: An underappreciated "two-edged sword"

Pharmacological Research, Journal Year: 2025, Volume and Issue: 214, P. 107685 - 107685

Published: March 5, 2025

Ischemic stroke (IS) is an important cause of death and disability worldwide, but the molecular mechanisms involved are not fully understood. In this context, choline metabolism plays increasingly role in IS due to its multifaceted involving neuroprotection, neuroregeneration, inflammatory response, immune regulation, long-term health effects. With deepening research on metabolites, such as trimethylamine-N-oxide (TMAO), scientists have gradually realized key occurrence, development potential treatment IS. This review summarizes importance neuroprotection disease management, highlighting complexity affecting cerebrovascular through gut microbes. Although metabolites exhibit a protective effect, excessive intake increases some may confer risk, suggesting need carefully balance dietary intake. The purpose integrate existing results provide theoretical basis for further exploring mechanism, prognosis evaluation clinical intervention ischemic IS, hoping new perspective enlightenment formulation effective prevention strategies, promote comprehensive understanding heart brain optimize methods.

Language: Английский

---

New Nanoparticle-Based Drug: A 2024 Update and Future Prospects

BioNanoScience, Journal Year: 2025, Volume and Issue: 15(3)

Published: May 19, 2025

Language: Английский

---

Avicularin Treatment Ameliorates Ischemic Stroke Damage by Regulating Microglia Polarization and its Exosomes via the NLRP3 Pathway

Journal of Integrative Neuroscience, Journal Year: 2024, Volume and Issue: 23(11)

Published: Oct. 30, 2024

Background: Avicularin (AL), an ingredient of Banxia, has anti-inflammatory properties in cerebral disease and regulates polarization macrophages, but its effects on ischemic stroke (IS) damage have not been studied. Methods: In vivo, AL was administered by oral gavage to middle artery occlusion/reperfusion (MCAO/R) C57BL/6J mice doses 1.25, 2.5, 5 mg/kg/day for seven days, and, vitro, added treat oxygen-glucose deprivation (OGD)-BV2 cells. Modified neurological severity score, Triphenyltetrazolium chloride (TTC) staining, brain-water-content detection, TdT-mediated dUTP nick-end labeling (TUNEL) assay, flow cytometry, immunofluorescence Enzyme linked immunosorbent assay (ELISA), Western-blot analysis were used investigate the functions mechanism effect treatment IS. The exosomes AL-treated microglia studied transmission electron microscope (TEM), nanoparticle tracking analyzer (NTA), analysis. Results: reduced infarct volume, brain-water content, neuronal apoptosis, release inflammatory factors, that induced MCAO/R. Notably, M2 promoted M1 inhibited penumbra MCAO/R mice. Subsequently, polarization-regulating verified vitro. Suppressed NOD-like receptor thermal protein domain associated 3 (NLRP3) inflammasome activation found animal Oxygen-Glucose Deprivation/Reoxygenation (OGD/R) cells treated with AL, as evidenced decreasing NLRP3-inflammasome-related downstream factors. After treatment, anti-apoptosis microglial OGD/R primary cortical neurons increased. Conclusion: reduce responses neuron death IS-associated models regulating NLRP3 pathway affecting exosomes.

Language: Английский

---

Unraveling the impact of different liposomal formulations on the plasma protein corona composition might give hints on targeting capability of nanoparticles

Nanoscale Advances, Journal Year: 2024, Volume and Issue: 6(17), P. 4434 - 4449

Published: Jan. 1, 2024

Nanoparticles (NPs) interact with biological fluids after being injected into the bloodstream. The interactions between NPs and plasma proteins at nano-bio interface affect their biopharmaceutical properties distribution in organ tissues due to protein corona (PrC) composition, turn, modification of resulting targeting capability. Moreover, lipid polymer NPs, interface, composition PrC relative adsorption abundance specific proteins. To investigate this latter aspect, we synthesized characterized different liposomal formulations (LFs) lipids polymer-conjugated molar ratios, having sizes, size distributions surface charges. various designed LFs was evaluated

Language: Английский

---

Metabolomics study of APETx2 post-conditioning on myocardial ischemia-reperfusion injury

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 6, 2024

Acid-sensing ion channels are activated during myocardial ischemia and implicated in the mechanism of ischemia-reperfusion injury (MIRI). channel 3 (ASIC3), most pH-sensitive member ASIC family, is highly expressed tissues. However, role ASIC3 MIRI its precise effects on metabolome remain unclear. These unknowns might be related to cardioprotective observed with APETx2 post-conditioning. Rat hearts subjected Langendorff perfusion were randomly assigned normal (Nor) group, ischemia/reperfusion (I/R) blockade (AP) group. group AP treated ASIC3-specific inhibitor (630 nM). Molecular morphological changes elucidate MIRI. Bioinformatics analyses identified differential metabolites pathways associated post-conditioning stabilized hemodynamics isolated rat heart model It also reduced infarct size, mitigated mitochondrial damage at ultrastructural level, improved markers oxidative stress. Further more, we that phosphatidylcholine, phosphatidylethanolamine, citric acid, cyanidin 5-O-beta-D-glucoside, L-aspartic acid decreased after The levels these partially restored by primarily involved autophagy endogenous cannabinoid signaling pathways. potentially a key player may improve through specific metabolic changes. This study provides valuable data for future research mechanisms underlying

Language: Английский

---