Insulin for oral bone tissue engineering: a review on innovations in targeted insulin-loaded nanocarrier scaffold DOI

Jiyaur Rahaman,

Dhrubojyoti Mukherjee

Journal of drug targeting, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 31

Published: Dec. 21, 2024

The occurrence of oral bone tissue degeneration and defects by osteoporosis, tooth extraction, obesity, trauma, periodontitis are major challenges for clinicians. Traditional regeneration methods often come with limitations such as donor site morbidity, limitation special shape, inflammation, resorption the implanted bone. treatment oriented biomimetic materials has achieved significant attention recently. In engineering arena, insulin gained considerable among all known biomaterials osteogenesis angiogenesis. It also exhibits osteogenic angiogenic properties interacting receptors on osteoblasts. Insulin influences remodelling both directly indirectly. acts through PI3K/Akt MAPK signalling pathways indirectly modulating RANK/RANKL/OPG pathway, which helps reduce resorption. current review reports role in cavity form scaffolds nanomaterials. Different delivery systems, utilising nanomaterials functionalised polymeric have been explored regeneration. put forward a theoretical basis future research composite

Language: Английский

Pegylated Graphene Oxide For 4′‐Fluorouridine Delivery: An Ab Initio Approach to Antiviral Therapy DOI Creative Commons
Oluwasegun Chijioke Adekoya, Gbolahan Joseph Adekoya, Wanjun Liu

et al.

Advanced Theory and Simulations, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 26, 2024

Abstract Effective antiviral drug delivery systems, such as for 4′‐Fluorouridine (4′‐FlU), are crucial addressing viral infections like COVID‐19. This study used ab‐initio analysis to examine interactions between 4′‐FlU and graphene oxide (GO)‐based carriers, with without polyethylene glycol (PEG) functionalization, across various physiological conditions. Non‐PEGylated GO showed strong gas‐phase binding (−103.36 kcal mol −1 ), supporting systemic stability, while PEGylation reduced aqueous‐phase (−33.72 enhancing biocompatibility circulation. Adsorption energies were significant in acidic alkaline environments, intensifying (−118.42 ). Charge transfer dynamics, influenced by pH PEGylation, revealed enhanced stability under conditions, suitable tumor microenvironments. Hydrogen bonding stabilized GO‐drug complexes, ensuring prolonged release. PEGylated excelled especially delivery, confirmed miscibility studies controlled release kinetics. Thermodynamic quantum chemical analyses highlighted environmental factors optimizing reactivity. GO/PEG‐4′‐FlU is ideal localized tumors, GO/4′‐FlU supports requiring broader dispersion. research lays the groundwork nanotechnology‐based strategies design of adaptable systems both applications.

Language: Английский

Citations

4
When conventional approach in toxicity assays falls short for nanomedicines: a case study with nanoemulsions DOI Creative Commons
Ines Nikolić, Jelena Đoković, Dóra Méhn

et al.

Drug Delivery and Translational Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Abstract The aim of this study was to assess the critical quality attributes parenteral nanoemulsion formulations by measuring several physicochemical parameters and linking them their in vitro performance, illustrating how simplistic routinely used approaches are insufficient for understanding a potential nanomedicine. Physicochemical characterization should encompass size distribution through at least two orthogonal techniques, such as dynamic light scattering (DLS) electron microscopy, with added value from analytical ultracentrifugation. In toxicity assessment performed using three different assays determine mitochondrial activity (WST-1), membrane integrity (lactate dehydrogenase release (LDH) assay), cell viability (propidium iodide (PI) staining). Special focus placed on estimating appropriate incubation times relevant results biological investigations. All had an average diameter around 100 nm. Conclusions regarding safety were assay-dependent: LDH PI-based showed good correlation, while WST-1 assay indicated that non-PEGylated formulation altered more significantly compared PEGylated ones. underlined selection cytotoxicity be based possible mechanism cellular perturbation. Alternatively, aspects tested. Additionally, there is need well-designed controls overcome nanoparticle effects avoid potentially false high results, which demonstrated. Combining orthogonal, standardized manner initial step reliable preclinical nanomedicines suggested. This represents key aspect new methodologies nanomedicine characterization. Graphical abstract

Language: Английский

Citations

0
Optimal size and PEG coating of gold nanoparticles for prolonged blood circulation: a statistical analysis of published data DOI Creative Commons
Dmitry Nevozhay,

Ronald A. Rauch,

Zhongya Wang

et al.

Nanoscale Advances, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

A statistical meta-analysis of gold nanoparticles reveals non-linear interactions. The optimal design (diameter ≤ 40 nm and PEG ≥ 5 kDa) maximizes blood circulation, providing evidence-based guidelines for enhanced nanoparticle in biomedicine.

Language: Английский

Citations

0
Biodistribution of 89Zr-Radiolabeled Nanoassemblies for Monoclonal Antibody Delivery Revealed through In Vivo PET Imaging DOI Creative Commons
Ana M. López-Estévez, Amaia Carrascal-Miniño, Dolores Torres

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Despite the outstanding performance of monoclonal antibodies (mAbs) in clinic, their full potential has been hindered due to inability cross cell membranes and therefore reach intracellular targets. The use nanotechnology deliver mAbs domains highlighted as a strategy with high potential. Working toward this goal, we have recently developed validated palmitoyl hyaluronate (HAC16)-based nanoassemblies (HANAs), novel technology for delivery Kirsten Rat Sarcoma Virus (KRAS)-mutated tumors, one most prevalent challenging oncoprotein. success, pharmacokinetics biodistribution these vehicles are still unknown chemical complexity, challenge common large proportion drug nanomedicines. To support further development clinical translation, present an efficient radiolabeling approach positron emitter zirconium-89 (89Zr) vivo evaluation HANAs by whole-body PET imaging. Additionally, assessed impact PEGylation size modulation on profile using 89Zr-radiolabeled PEGylated non-PEGylated HANAs. Our imaging results demonstrated that significantly modify 89Zr-mAb. Furthermore, established can be conveniently modulated introducing PEG polymers surface, facilitating customization cancer applications. This versatile provides facile complex nanoformulations loaded mAbs, quantitative manner sensitivity.

Language: Английский

Citations

0
Innovative microfluidic model for investigating the intestinal mucus barrier: numerical and experimental perspectives DOI Creative Commons
Mohammad Valibeknejad, Reza Alizadeh, S. Majid Abdoli

et al.

Drug Delivery and Translational Research, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

The intestinal mucus layer serves as a critical first line of defense against external agents, functioning barrier to the absorption drugs, food, and pathogens. While numerous in vitro studies have explored role preventing particle penetration, effects flowing luminal material, dislodging because induced shear rate by lumen material interfacial phenomena remain poorly understood. This study introduces microfluidic approach simulate interaction between layer. successfully measures both penetration into dislodgement material. A biosimilar model (BSM) Hank's Balanced Salt Solution (HBSS) were employed mimics human fluid, respectively. To investigate effect viscosity on pattern, two variants used: BSM-1, representing low-viscosity model, BSM-2, high-viscosity model. velocity fields extracted tracking fluorescent particles. results revealed significant differences BSM-1 attributed their rheological properties. These findings further confirmed through an assessment viscoelastic properties BSM models. utilized COMSOL Multiphysics for numerical simulations, predicting experimental outcomes solving fluid flow equations. Physicochemical characterizations HBSS performed link with including sweep tests, application power-law viscosity, measurements density wettability. proposes platform examining low- offer valuable insights barrier's response stress. validated physicochemical provide foundation future rates more complex geometries diverse conditions.

Language: Английский

Citations

0
Nanotechnology-based drug delivery for breast cancer treatment: Current applications and future directions DOI Creative Commons
Md Abdus Samad, Iftikhar Ahmad, Torki A. Zughaibi

et al.

European Journal of Medicinal Chemistry Reports, Journal Year: 2025, Volume and Issue: unknown, P. 100268 - 100268

Published: April 1, 2025

Language: Английский

Citations

0
PEGylation-Dependent Cell Uptake of Lipid Nanoparticles Revealed by Spatiotemporal Correlation Spectroscopy DOI
Luca Digiacomo, Serena Renzi,

Andrea Pirrottina

et al.

ACS Pharmacology & Translational Science, Journal Year: 2024, Volume and Issue: 7(10), P. 3004 - 3010

Published: Sept. 5, 2024

Polyethylene glycol (PEG) is a common surface modification for lipid nanoparticles (LNPs) to improve their stability and in vivo circulation time. However, the impact of PEGylation on LNP cellular uptake remains poorly understood. To tackle this issue, we systematically compared plain PEGylated LNPs by combining dynamic light scattering, electrophoretic synchrotron small-angle X-ray scattering (SAXS) that unveils striking similarity size core structure but significant reduction charge. Upon administration human embryonic kidney (HEK 293) cells, were internalized through different endocytic routes, as revealed spatiotemporal correlation spectroscopy. An imaging-derived mean square displacement (iMSD) analysis shows exhibit significantly stronger preference caveolae-mediated endocytosis (CAV) clathrin-mediated (CME) pathways LNPs, with these latter being better tailored MCR-dependent internalization trafficking. This suggests PEG plays crucial role directing toward specific routes. Further studies should explore how PEG-mediated impacts intracellular trafficking ultimately translates therapeutic efficacy, guiding design next-generation delivery systems.

Language: Английский

Citations

2
Exploring Micelles and Nanospheres as Delivery Systems for Phenothiazine Derivatives in Cancer Therapy DOI Creative Commons
Katarzyna Jelonek, Monika Musiał‐Kulik, Małgorzata Pastusiak

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(12), P. 1597 - 1597

Published: Dec. 16, 2024

Objectives: Cancer remains one of the leading causes death worldwide, and thus, there is a need for development innovative more effective treatment strategies. The aim study was to evaluate two types nanoparticles—nanospheres micelles—obtained from PLA-based polymers discover their potential delivering four phenothiazine derivatives. Methods: morphology, drug-loading properties, cytocompatibility, hemolytic properties anticancer activity were analyzed. Results: micelles exhibited significantly higher release process cytotoxic against cancer cells compared nanospheres. containing 5-methyl-12H-quino[3,4-b][1,4]benzothiazinium chloride with an OH group as substituent in 10-position quinobenzothiazine ring showed highest content, most efficient drug release, lowest significant effect HeLa cells. Conclusions: conducted enabled delivery system new compound that choice carrier has crucial on its

Language: Английский

Citations

0
Insulin for oral bone tissue engineering: a review on innovations in targeted insulin-loaded nanocarrier scaffold DOI

Jiyaur Rahaman,

Dhrubojyoti Mukherjee

Journal of drug targeting, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 31

Published: Dec. 21, 2024

The occurrence of oral bone tissue degeneration and defects by osteoporosis, tooth extraction, obesity, trauma, periodontitis are major challenges for clinicians. Traditional regeneration methods often come with limitations such as donor site morbidity, limitation special shape, inflammation, resorption the implanted bone. treatment oriented biomimetic materials has achieved significant attention recently. In engineering arena, insulin gained considerable among all known biomaterials osteogenesis angiogenesis. It also exhibits osteogenic angiogenic properties interacting receptors on osteoblasts. Insulin influences remodelling both directly indirectly. acts through PI3K/Akt MAPK signalling pathways indirectly modulating RANK/RANKL/OPG pathway, which helps reduce resorption. current review reports role in cavity form scaffolds nanomaterials. Different delivery systems, utilising nanomaterials functionalised polymeric have been explored regeneration. put forward a theoretical basis future research composite

Language: Английский

Citations

0