Development and validation of PCR marker array for molecular selection towards spring, vernalization-independent and winter, vernalization-responsive ecotypes of white lupin (Lupinus albus L.)

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 21, 2025

Abstract White lupin ( Lupinus albus L.) is an ancient grain legume that still undergoing improvement of domestication traits, including vernalization-responsiveness, providing frost tolerance and preventing winter flowering in autumn-sowing agriculture, vernalization-independence, conferring drought escape by rapid spring-sowing. A recent genome-wide association study highlighted several loci significantly associated with the most contrasting phenotypes, deletions promoter FLOWERING LOCUS T homolog, LalbFTc1 , some DArT-seq/silicoDArT loci. The present aimed to develop validate a versatile PCR marker array enabling molecular selection spring- winter-type white ecotypes. Candidate DArT-seq silicoDArT were transformed into cleaved amplified polymorphic sequence (CAPS) or derived CAPS markers. Developed markers, together those previously published for INDELs quantitative trait from linkage maps, implemented screening germplasm panel subjected 2-year phenotyping phenology traits. Three DArT-seq, two seven INDEL markers positively validated, constituting convenient PCR-based assay accurate reselection towards early thermoneutrality late as well tracking high genetic phenotypic diversity within landraces, revealed study.

Language: Английский

Molecular dissection of the genetic architecture of phenology underlying Lupinus hispanicus early flowering and adaptation to winter- or spring sowing

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: May 2, 2025

Spanish lupin, Lupinus hispanicus Boiss. et Reut. is an untapped grain legume species characterized by moderate frost resistance, tolerance to poor soil and waterlogging, high yield stability, remarkable seed protein content. It has been recognized as a good candidate for domestication broaden the palette of crop diversity. One key characteristics that need be precisely addressed during vernalization responsiveness flowering, which advantageous in autumn sowing due improved tolerance, whilst undesired spring it delays flowering. Ahead L. breeding, present work, we aimed recognize existing phenotypic variability flowering time response evaluate genetic architecture early late phenology DArT-seq genotyping genome-wide association study (GWAS) world germplasm collection species. Controlled environment phenotyping revealed significant correlations with population structure. yielded 23 728 highly polymorphic markers distributed extensively across all 26 chromosomes. GWAS identified number significantly associated or without pre-sowing vernalization, including those overlapping two major quantitative trait loci reported previously white lupin Microsynteny-based analysis content genome regions carrying highlighted several genes from photoperiodic pathways. To summarize, resources autumn- spring-sown cultivation provided tools marker-assisted selection towards required phenology.

Language: Английский

Stability of Early Maturing Soybean Genotypes in Poland

Agriculture, Journal Year: 2024, Volume and Issue: 14(12), P. 2202 - 2202

Published: Dec. 2, 2024

Poland is one of the northernmost countries in world where soybean breeding actively conducted. A large collection genotypes was analyzed three locations during 2022–2023 growing seasons. The objective this research to determine stability yield, as well protein and fat content seeds 280 genotypes. phenotypic correlations across environments were assessed using Spearman coefficient. statistical analysis provided additive main effects multiplicative interaction model. weighted average absolute scores. factor differentiating results between variation weather conditions. variety Karok, which ranks among top 10% genotype selection indices for yield thousand-seed weight, recommended based on studies conducted within Post-Registration Variety Testing System voivodeships Poland. Among most stable genotypes, terms traits paper, are many registered currently grown varieties such Obelix Pompei, Coraline, Kapral, ES Mentor, NS Mercury, Pepita, Pollux, Silesia, Heihe 45. development new should be factors by breeders.

Language: Английский