Hyperglycemia‐Enhanced Neutrophil Extracellular Traps Drive Mucosal Immunopathology at the Oral Barrier DOI Creative Commons
Qian Wang, Weimin Lin, Kexin Lei

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 5, 2024

Abstract Type 2 diabetes (T2D) is a risk factor for mucosal homeostasis and enhances the susceptibility to inflammation, in which neutrophils have been increasingly appreciated their role. Here, barrier disruption inflammation are observed at oral mucosa (gingiva) of T2D patients mice. It demonstrated that infiltrate gingival mice expel obvious neutrophil extracellular traps (NETs), while removal NETs alleviates barrier. Mechanistically, released dependent metabolic reprogramming. Under hyperglycemic condition, elevate both glucose incorporation glycolysis via increased expression GLUT1. Moreover, significantly levels local lesions patients, associated with clinical disease severity. This work elucidates causative link between hyperglycemia immunopathology, mediated by altered immuno‐metabolic axis neutrophil, thereby suggesting potential therapeutic strategy.

Language: Английский

Recent advances of hydrogels as smart dressings for diabetic wounds DOI
Xu Wang, Yuhan Yang, Weifeng Zhao

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(5), P. 1126 - 1148

Published: Jan. 1, 2024

This review highlights the latest progress of smart hydrogel dressings for bandaging, monitoring and therapy diabetic wounds.

Language: Английский

Citations

18
Endogenous/exogenous stimuli‐responsive smart hydrogels for diabetic wound healing DOI Creative Commons
Saadullah Khattak, Ihsan Ullah, Muhammad Sohail

et al.

Aggregate, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 6, 2024

Abstract Diabetes significantly impairs the body's wound‐healing capabilities, leading to chronic, infection‐prone wounds. These wounds are characterized by hyperglycemia, inflammation, hypoxia, variable pH levels, increased matrix metalloproteinase activity, oxidative stress, and bacterial colonization. complex conditions complicate effective wound management, prompting development of advanced diabetic care strategies that exploit specific characteristics such as acidic pH, high glucose stress trigger controlled drug release, thereby enhancing therapeutic effects dressings. Among solutions, hydrogels emerge promising due their stimuli‐responsive nature, making them highly for managing these The latest advancements in mono/multi‐stimuli‐responsive smart showcase superiority potential healthcare materials, highlighted relevant case studies. However, traditional dressings fall short meeting nuanced needs wounds, adjustable adhesion, easy removal, real‐time status monitoring, dynamic release adjustment according wound's conditions. Responsive represent a significant leap forward proficient sensing responding environment, offering more targeted approach treatment. This review highlights recent dressing, delivery, emphasizing role improving healing. It addresses ongoing challenges future directions, aiming guide clinical adoption.

Language: Английский

Citations

9
The Potential of DC Immunotherapy in Improving Proteinuria in Type 2 Diabetes Mellitus DOI Open Access
Jonny Jonny, Enda Cindylosa Sitepu,

I Nyoman Ehrich Lister

et al.

Published: July 10, 2024

Diabetic Kidney Disease (DKD) is a common complication of Type 2 Diabetes Mellitus and major risk factor for end-stage kidney disease. The immune system plays significant role in the pathogenesis DKD, involving infiltration cells kidney, upregulation in-flammatory mediators, downregulation anti-inflammatory disturbance ad-hesion molecules growth factors. Dendritic are professional antigen-presenting acting as bridge connecting innate adaptive responses. DC also capable modulating inflammation. Autologous dendritic can be made by vitro differentiation peripheral blood monocytes then utilized cell-based therapy. Cell-based therapy DKD currently focused on utilizing properties stem cells. However, research suggests unsatisfactory results due to several limitations. This article highlights possibility using through its controlling

Language: Английский

Citations

6
Single cell autofluorescence imaging reveals immediate metabolic shifts of neutrophils with activation across biological systems DOI Open Access
Rupsa Datta, Veronika Miskolci, Gina M. Gallego-López

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 29, 2024

Neutrophils, the most abundant leukocytes in human peripheral circulation, are crucial for innate immune response. They typically quiescent but rapidly activate response to infection and inflammation, performing diverse functions such as oxidative burst, phagocytosis, NETosis, which require significant metabolic adaptation. Deeper insights into changes will help identify regulation of neutrophil health diseases. Due their short lifespan associated technical challenges, processes neutrophils not completely understood. This study uses optical imaging (OMI), entails redox ratio fluorescence lifetime microscopy intrinsic coenzymes NAD(P)H FAD assess state single neutrophils. Primary were imaged

Language: Английский

Citations

5
Single-cell analysis of age-related changes in leukocytes of diabetic mouse hindpaws DOI Creative Commons
James M. Nichols,

Hoang Vu Pham,

Eric Lee

et al.

Cellular and Molecular Life Sciences, Journal Year: 2024, Volume and Issue: 81(1)

Published: March 19, 2024

Abstract Complications associated with Type 1 and 2 diabetes, such as diabetic peripheral neuropathy foot ulcers, are a growing health-care concern. In addition, this concern increases patients age due to their increased susceptibility complications. To address problem, it is important understand fluctuations in physiology which lead pathological changes the metabolic disturbances of diabetes. Our study explores dysregulation immune cell populations hindpaws healthy mice at 12 21 weeks using single-cell RNA sequencing provide insight into disruptions occurring distal limb during chronic 21-week-old Lepr db/db mice, were seen mast cells/basophils, dermal γδ T cells, heterogeneous innate lymphoid cells. macrophages represented largest cluster cells showed greatest increase genes immune-specific pathways. Sub-clustering revealed bias toward angiogenic Lyve1 + MHCII lo corresponded an on histology. results show that immunological function phenotype multiple types shift not only disturbance, but also duration disease, may explain pathologies more

Language: Английский

Citations

4
The impact of aging on neutrophil functions and the contribution to periodontitis DOI Creative Commons
Zi Wang,

Anish Saxena,

Wenbo Yan

et al.

International Journal of Oral Science, Journal Year: 2025, Volume and Issue: 17(1)

Published: Jan. 16, 2025

Language: Английский

Citations

0
Exploring neutrophils as therapeutic targets in cardiometabolic diseases DOI
Mattia Albiero, Andrea Baragetti

Trends in Pharmacological Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Delayed Immune Response Upon Injury in Diabetic Wounds Impedes Healing DOI Creative Commons
Priyanka Dhanraj, Kiara Boodhoo, Marí van de Vyver

et al.

Immunity Inflammation and Disease, Journal Year: 2025, Volume and Issue: 13(2)

Published: Jan. 31, 2025

Chronic wounds are a severe complication of diabetes. Dysregulated inflammatory signalling is thought to underly the poor healing outcomes. Yet, there little information available on acute response following injury and its impact healing. Using murine full thickness excisional wound model, current study therefore assessed expression pro-inflammatory pro-resolving lipid mediators during early stages post in diabetic compared timeframe for transitioning through phases Tissue eicosanoid (LTB4, PGE2, TxA2, MaR1, RvE1, RvD1, PD) MMP-9 levels were at 6 h wounding using ELISAs. Wound closure, dynamics (histology), cellular infiltration MPO, TNF-α (IHC) h, day2, day7 wounding. Eicosanoid did not differ between groups (LTB4 24-125 pg/mL, PGE2 63-177 TxA2 529-1184 MaR1 365-2052 RvE1 43-1157 RvD1 1.5-69 PD1 11.5-4.9 ng/mL). An inverse relationship (p < 0.05) eicosanoids however only evident wounds. Diminished (x5 fold) coincided with significant delay (pro-inflammatory cytokine) MPO (neutrophil marker). A difference (C 1.8 ± 0.6; DM 0.7 0.1 MFI) 4.9 1.9; 0.9 0.4 was observed as wounding, histology parameters supporting notion that onset delayed These observations imply immune cells unresponsive initial tissue.

Language: Английский

Citations

0
Predicting 90-day risk of urinary tract infections following urostomy in bladder cancer patients using machine learning and explainability DOI Creative Commons
Qi Zhao, Mengyao Liu, Kuang‐Hong Gao

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 25, 2025

This research aims to design and validate a machine learning model predict the probability of urinary tract infections within 90 days post-urostomy in bladder cancer patients. Clinical follow-up information from 317 patients who had urostomy procedures at First Affiliated Hospital Shanxi Medical University (May 2018–May 2024) were analyzed. The dataset partitioned into training testing sets, feature selection was executed via Least Absolute Shrinkage Selection Operator regression technique. Seven algorithms employed: Logistic Regression, K-Nearest Neighbors, LightGBM, Random Forest, XGBoost, Support Vector Machine, Multi-Layer Perceptron. Performance metrics for assessed using multiple evaluation indicators, including AUC, accuracy, sensitivity, specificity, Positive Predictive Value, Negative F1 score. SHapley Additive exPlanations Local Interpretable Model-agnostic Explanations applied interpretability. UTIs occurred 22.08% after urostomy. predictive pinpointed eight important clinical features. Among developed models, SVM demonstrated best overall performance with AUC (0.835), accuracy (0.825), precision (0.583), recall (0.778), score (0.667). model, designed assess risk patients, has been deployed online healthcare professionals at: https://zqmodel.shinyapps.io/shinydashboard_model/ .

Language: Английский

Citations

0
XueBiJing injection reduced mortality in sepsis patients with diabetes DOI Creative Commons
Yan Liu,

Hengheng Dai,

Yixuan Li

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 27, 2025

Sepsis patients with diabetes are at a high clinical risk. It is well reported that XueBiJing injection has good benefit in sepsis individuals. However, there no relevant report about the efficacy and safety of XBJ comorbid diabetes. Data two large randomized controlled trials (XBJ-SAP (ChiCTR-TRC-13003534) EXIT-SEP (NCT0323874)) were combined, post hoc analyses performed. further divided into XBJ-treated group placebo based on inclusion exclusion criteria. The primary (28-day mortality) secondary outcomes (mortality ICU post-randomization hospital, acute physiology, chronic health evaluation II (APACHE II) score sequential organ failure assessment (SOFA) score) compared between treatment groups status baseline. Moreover, occurrence adverse events (AEs) was also assessed. At study baseline, total 378 (227 men [60.0%] 151 women [40.0%]; mean [SD] age, 60.3 [11.1] years) considered to have diabetes, which 177 received 201 administration. Among these mortality 28 days significantly lower than (29 173 [16.8%] vs. 56 198 [28.3%], P = 0.01), absolute risk difference 11.5% (95% CI, 3.1%-19.9%). Furthermore, overall incidence when used (24.4% [42 172 patients] 27.7% [54 195 patients]. present underscores pivotal role modulating immune response among suffering from mellitus, exploring positive effects mellitus. those consistent trial findings, demonstrating efficacious suggesting need for special precautions. ChiCTR-TRC-13003534 NCT0323874.

Language: Английский

Citations

0