DNA isolation in bark beetles: reliability of extraction methods and application in downstream molecular procedures DOI Creative Commons
Leonel Stazione, María Victoria Lantschner, Juan C. Corley

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 9, 2024

Abstract Molecular tools are increasingly used in entomology for several applications such as taxonomy, genetics, ecology and evolution. For these studies, DNA extraction from individual insects is a crucial step, since the yield quality vary depending on methods used. Finding an ideal balance between particularly difficult to reach constraints must be considered upon selecting final protocol. Bark beetles (Coleoptera: Curculionidae, Scolytinae) diverse group of forest which molecular studies at level important, given that many species invasive may become pests. However, bark not easy, their small size complex molecules nature conforming exoskeleton present digestive tract. Here, we carried out comparative analysis performance five beetle species: Hylurgus ligniperda, Hylastes ater, Orthotomicus erosus, laricis Cyrtogenius luteus . By assessing efficiency two different protocols, our aim was establish best species-specific method population-level studies. Our results showed method’s whole mainly depends translates into quantity variance. We also noted traditional better PCR smallest whereas commercial kit performed larger beetles. provides evidence no single isolation best, each particular requires optimization.

Language: Английский

Reconstructing the invasion pathways of the bark beetles Orthotomicus erosus and Cyrtogenius luteus (Coleoptera, Curculionidae, Scolytinae) in South America DOI
Leonel Stazione, Carolina Soliani, Anthony I. Cognato

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Abstract Global trade and the spread of non-native host plants are driving introduction forest insects to new regions, often causing substantial ecological economic damage in forests. Understanding invasion pathways is essential tracking preventing these introductions. This study investigates two pine bark beetle species (Orthotomicus erosus Cyrtogenius luteus) South America using mitochondrial DNA analysis. We collected samples from native ranges (Europe/Asia) invaded areas (Argentina/Uruguay) understand genetic diversity likely routes. For O. erosus, results revealed main clusters its range, with American populations closely related western European populations. The presence shared haplotypes between Argentina Uruguay timeline, suggest a single event, through first. C. luteus, one widespread haplotype was found across all populations, indicating China as possible source, though multiple introductions may have occurred given unique America. Both show similar patterns Uruguay, suggesting connected facilitated by extensive plantations regional trade. highlights importance understanding for implementing effective biosecurity measures, particularly expected increase pest invasions over coming decades.

Language: Английский

Citations

0
DNA isolation in bark beetles: reliability of extraction methods and application in downstream molecular procedures DOI Creative Commons
Leonel Stazione, María Victoria Lantschner, Juan C. Corley

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 9, 2024

Abstract Molecular tools are increasingly used in entomology for several applications such as taxonomy, genetics, ecology and evolution. For these studies, DNA extraction from individual insects is a crucial step, since the yield quality vary depending on methods used. Finding an ideal balance between particularly difficult to reach constraints must be considered upon selecting final protocol. Bark beetles (Coleoptera: Curculionidae, Scolytinae) diverse group of forest which molecular studies at level important, given that many species invasive may become pests. However, bark not easy, their small size complex molecules nature conforming exoskeleton present digestive tract. Here, we carried out comparative analysis performance five beetle species: Hylurgus ligniperda, Hylastes ater, Orthotomicus erosus, laricis Cyrtogenius luteus . By assessing efficiency two different protocols, our aim was establish best species-specific method population-level studies. Our results showed method’s whole mainly depends translates into quantity variance. We also noted traditional better PCR smallest whereas commercial kit performed larger beetles. provides evidence no single isolation best, each particular requires optimization.

Language: Английский

Citations

1