Target Enzymes of Origanum majorana and Rosmarinus officinalis Essential Oils in Black Cutworm (Agrotis ipsilon): In Vitro and In Silico Studies

Insects, Journal Year: 2024, Volume and Issue: 15(7), P. 483 - 483

Published: June 28, 2024

In this study, in vitro and silico approaches were employed to assess the toxicity of marjoram (Origanum majorana) rosemary (Rosmarinus officinalis) essential oils (EOs) A. ipsilon larvae. The study determined activities ATPases larvae after treatment with LC20 LC70 each EO. α-esterase glutathione-S-transferase (GST) also LC10 LC30 Furthermore, molecular docking was determine binding affinity terpinene-4-ol α-pinene, major constituents O. majorana, R. officinalis EOs, respectively, compared co-crystallized ligand α-esterase, diethyl hydrogen phosphate (DPF). Toxicity assays revealed that majorana EO more toxic than at 96 h post-treatment. However, latter significantly inhibited activity Na+-K+ pump almost all intervals. same concentrations Mg2+/Ca2+-ATPase Ca2+ contrast, showed a variable effect on across different time On other hand, both EOs varied effects GST over time. Molecular energy scores −4.51 −4.29 kcal/mol for score −4.67 PDF. Our demonstrated tested ipsilon, suggesting their potential efficacy as insecticides.

Language: Английский

---

Monitoring and Detection of Insecticide Resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae): Evidence for Field-Evolved Resistance in Egypt

Insects, Journal Year: 2024, Volume and Issue: 15(9), P. 705 - 705

Published: Sept. 16, 2024

Spodoptera frugiperda (J.E. Smith) (Noctuidae: Lepidoptera) is a notable insect pest that invades major cereal crops, causing significant damage and loss. Resistances of 2nd instar larvae two Egyptian field populations S. frugiperda, collected from the Fayoum Giza governments, were measured against eight insecticides, including traditional insecticides (profenofos cypermethrin), bio-insecticides (emamectin benzoate, spinosad, Bacillus thuringiensis), growth regulators (IGRs) (lufenuron, diflubenzuron, methoxyfenozide). In addition, synergistic effects three synergists (Piperonyl butoxide (PBO), diethyl maleate (DEM), triphenyl phosphate (TPP) assessed, activities detoxification enzymes (acetylcholine esterase (AChE), cytochrome P-450 (CYP-450), carboxylesterase (CarE), glutathione-s-transferase (GST) also determined. Resistance surveillance revealed population showed moderate resistance to cypermethrin (RR = 5.75-fold), followed by spinosad 2.62-fold), lufenuron (2.01-fold). On other hand, exhibited only 3.65-fold). Our results emamectin benzoate was most effective insecticide, with an LC50 value 0.003 mg/L for 0.001 population, compared susceptible strain (0.005 mg/L). Among biological thuringiensis least toxic insecticide all tested strains. Synergism assays indicated DEM TPP had effect on (SR 8.00-fold both), PBO 5.71-fold) alone. The assay GST activity significantly (p < 0.05) increased in strains strain. However, no changes observed among CYP-450, CarE, or AChE. findings this study provide substantial insights into tracking managing development Egypt.

Language: Английский

---

Toxicity, Biochemical Impact, and Bioinformatics Analysis of Jujube Oil on Culex pipiens Larvae

Journal of Experimental Zoology Part A Ecological and Integrative Physiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Culex pipiens (Cx. pipiens) are ubiquitous vectors of public health pathogens responsible for numerous human diseases. Consequently, their management is imperative. So, this study aimed to assess the susceptibility third instar larvae Cx. distinct concentrations jujube oil, LC50 and LC90 were determined. Also, developmental duration immatures, percentage emerged adults, physiological responses at sublethal concentration recorded. GC-MS analysis was carried out characterize fractions oil. Additionally, bioinformatics utilized. The results showed that susceptible with larval mortality escalating in correlation increased oil exposure time. Sublethal significantly prolonged from 11 days control 15 treated. Conversely, adults decreased 88.89% 17.78%in data also indicated treatment activities acetylcholinesterase, glutathione S transferase, arginine kinase, cytochrome P450, ecdysone. titers catalase 3-hydroxykynurenine increased. identified 9-Octadecenoic acid, methyl ester, (E)-, Octadecanoic ester Hexadecanoic as predominant components. Bioinformatics assessments ecdysone, juvenile hormone, tryptophan oxygenase lowest binding affinity scores. In sum, supports potential an effective alternative botanical agent larvae.

Language: Английский

---

In vitro and Silico Exploration of the Insecticidal Properties of Lavandula multifida L. Essential Oil and its Binary Combinations with Cyantraniliprole and Emamectin Benzoate on Spodoptera frugiperda (Lepidoptera: Noctuidae)

Crop Protection, Journal Year: 2024, Volume and Issue: unknown, P. 106969 - 106969

Published: Oct. 1, 2024

Language: Английский

---

Bazı Lamiaceae Uçucu Yağlarının Tenebrio molitor Larvaları Üzerinde İnsektisidal Etkileri ve Larvaların Ana Yaşına Bağlı Duyarlılıkların Belirlenmesi

Akademik Ziraat Dergisi, Journal Year: 2024, Volume and Issue: 13(2), P. 287 - 297

Published: Dec. 30, 2024

Lamiaceae familyasına ait 6 farklı bitki uçucu yağının T. molitor larvalarına karşı öldürücü etkilerini araştırmak, en yüksek etkiye sahip yağlar ile popülasyonun yaş gruplarından meydana gelen larvalar üzerinde ölüm oranı farklılıklarını tespit etmek amaçlanmıştır. Materyal Yöntem: Melisa (Melissa officinalis), Kekik (Origanum minutiflorum), Nane (Mentha piperita), Adaçayı (Salvia Fesleğen (Ocimum basilicum), Lavanta (Lavandula intermedia)’ dan elde edilen yağların 3 dozu (5 ml, 3ml, 1ml L-1) larvaları daldırma ve kalıntı yöntemi test edilmiştir. İstatistiki olarak oranına yağın ml L-1 dozu, ana dönemlerinden getirdiği larvaların son dönemleri metoduyla denenmiştir. Bulgular: O. minutiflorum, M. piperita, yağlarının 5 officinalis dozları %100 ölüme sebep etkili yağ belirlenmiştir. Ayrıca bu üç anne bireylerin dönemlerinde ortaya çıkan 4 grup larva ‘in olduğu oranları sırasıyla; %86.6, %100, %73.3 minutiflorum sadece ilk grupta %64.4, piperita ise 4. %48.9 olup diğer gruplarda edilmemiştir. Bu çalışma sonucu canlılığını sürdüren tüm deneme karakterlerinde ikinci nesil oluşumu gözlenmiştir. Tüm öldürücülük etkisini doz özelinde gösteren tekrar geç dönem uygulanarak LC50 değeri,1.96 LC90 değeri 3,09 Sonuç: sahiptir. Denemede kullanılan düşük maliyetli daha dozlarıyla yapılacak yeni araştırmalara ihtiyaç duyulmaktadır. annelerin oranlarındaki farklılıklar, kurulacak olan denemelerde dişi kriterlerine dikkat edilmesi gerekliliğini göstermektedir.

---