The Inevitable Fate of Tetranychus urticae on Tomato Plants Treated with Entomopathogenic Fungi and Spinosad

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(2), P. 138 - 138

Published: Feb. 12, 2025

Tetranychus urticae (Acari: Tetranychidae) is a pervasive and damaging mite pest of tomato crops, leading to important economic losses globally. This study evaluated the acaricidal efficacy spinosad, alone in combination with Beauveria bassiana (Bb) WG-21 Metarhizium robertsii (Mr) WG-04, laboratory (application leaf discs) greenhouse plants), considering mortality establishment, respectively. The treatments Bb or Mr WG-04 spinosad achieved 100% T. nymphs within 2 days on discs, while individual applications each control agent resulted lower mortalities, ranging between 62.91 86.25% after 3 days. paired treatment + killed all exposed adults 5 d, that same results 7 d. However, ≥77.08% In greenhouse, deterred presence (adults, immatures, eggs) either surface leaves, suppressed populations only adaxial surface. These findings indicate combined tested EPF especially plants under conditions can provide substantially enhanced life stages compared applied alone.

Language: Английский

Biocontrol of Cassida vittata Vill. (Coleoptera: Chrysomelidae) in Sugar Beet Crops Using Streptomyces sp. Strains

Microbiology Research, Journal Year: 2025, Volume and Issue: 16(3), P. 57 - 57

Published: Feb. 28, 2025

Cassida vittata Vill. is a major pest of sugar beet crops worldwide. This study evaluated the efficacy Streptomyces sp. strains E23-2, E23-9, E23-3, and E25-12 in managing this under both laboratory bioassays (26 ± 2 °C, 60 10% RH, 12 h photoperiod) field conditions. In bioassays, insecticidal repellent activities were assessed using topical leaf dip methods. The activity test involved five concentrations bacterial suspensions (102 to 1010 cfu. mL−1 (Colony-Forming Units per milliliter)) against larvae adults. Only E23-2 E23-9 at 101⁰ 10⁸ mL−1, exhibiting highest activity, used for bioassay. Carbosulfan 0.25 g/L served as positive control. Results indicated E23-2’s high C. vittata, with lowest LC50 values: 323.5 (larvae) 5.1 × 103 (adults) contact, 1.9 3.1 104 method. LT50 values 3 days adults contact method supported efficacy. displayed notable repellency (RI = 84.9% 48 h). trials, Henderson–Tilton adjusted rates revealed substantial reductions 88.6% 85.9% (adults), aligning closely Carbosulfan’s Enzymatic analysis underscored versatile biocontrol attributes providing insights targeted management strategies. Field conditions, notably temperature, can influence establishment EP bacteria. Further studies are imperative comprehensive understanding these influencing factors.

Language: Английский

Effect of Rare, Locally Isolated Entomopathogenic Fungi on the Survival of Bactrocera oleae Pupae in Laboratory Soil Conditions

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 811 - 811

Published: April 2, 2025

Greece’s olive oil production is significantly affected by the fruit fly Bactrocera oleae (Diptera: Tephritidae), and its presence perceived when it too late to act for damage recovery. In this work, some unexplored entomopathogenic fungi (EPFs) were studied their efficacy on pupae in soil samples. Olive grove samples collected evaluate effect of EPFs natural environment. The parameters that analyzed performance B. included adult survival time, pupa hatch mycelium dead adults. was highlighted present adults after treating with fungal isolates substrate. results showed substrate, external growth observed A. contaminans, keveii, flavus P. lilacinum, T. annesophieae (100%). Remarkably, lowest male proportion non-soil substrates (0.41–0.42) first keveii (0.36), citreosulfuratum (0.41) soil-only substrate contrast control treatment (0.5 both substrates). Given high infestation caused flies Greece, study emphasize use incorporating certain EPF-based biopesticides into integrated pest management (IPM) programs.

Language: Английский