Cited by The Promise and Challenges of Bioprinting in Tissue Engineering

Recent Advances in Three-Dimensional In Vitro Models for Studies of Liver Fibrosis DOI

Kyun Yoo, Gyeongmin Kim, Jeong Sang Son

et al.

Tissue Engineering and Regenerative Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: May 13, 2025

Language: Английский

Citations

The preparation, modification and hepatoprotective activity of chitooligosaccharides: A review DOI

Peng Liu, Wanchao Chen, Di Wu

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 277, P. 134489 - 134489

Published: Aug. 5, 2024

Language: Английский

Citations

Bioinspired Bioinks for the Fabrication of Chemomechanically Relevant Standalone Disease Models of Hepatic Steatosis DOI

G Guagliano, Cristina Volpini, Lorenzo Sardelli

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(14)

Published: Feb. 7, 2024

Hepatotoxicity-related issues are poorly predicted during preclinical experimentation, as its relevance is limited by the inadequacy to screen all non-physiological subclasses of population. These pitfalls can be solved implementing complex in vitro models hepatic physiology and pathologies phase. To produce these platforms, extrusion-based bioprinting focused on, since it allows manufacture tridimensional cell-laden constructs with controlled geometries, a high-throughput manner. Different bioinks, whose formulation tailored mimic chemomechanical environment steatosis, most prevalent disorder worldwide, proposed. Internally crosslinked alginate hydrogels chosen structural components inks. Their viscoelastic properties (G' = 512-730 Pa G″ 94-276 Pa, depending on frequency) tuned those steatotic liver tissue. Porcine ECM introduced relevant biochemical cue. Sodium oleate added recall accumulation lipids Downstream analyses 14-layered bioprinted structures cultured for 10 days reveal establishment steatotic-like features (intracellular lipid vesicles, viability decrease up ≈50%) without needing external conditionings. The presented bioinks thus suitable fabricate steatosis implemented experimental frame.

Language: Английский

Citations

Dynamic, IPSC-derived hepatic tissue tri-culture system for the evaluation of liver physiology in vitro DOI

Benedikt Scheidecker, Stéphane Poulain, Masahiro Sugimoto

et al.

Biofabrication, Journal Year: 2024, Volume and Issue: 16(2), P. 025037 - 025037

Published: March 6, 2024

Abstract Availability of hepatic tissue for the investigation metabolic processes is severely limited. While primary hepatocytes or animal models are widely used in pharmacological applications, a change methodology towards more sustainable and ethical assays highly desirable. Stem cell derived cells generally regarded as viable alternative above model systems, if current limitations functionality maturation can be overcome. By combining microfluidic organ-on-a-chip technology with individually differentiated, multicellular fractions, we aim to improve overall hepatocyte-like cells, well evaluate cellular composition interactions non-parenchymal populations formation mature liver tissue. Utilizing multi-omic approach, show improved profiles maintained dynamic microenvironment compared standard culture setups without continuous perfusion. In order resulting tissue, employ single sequencing distinguish formed subpopulations spatial localization. input was strictly defined based on established differentiation protocols parenchyma, endothelial stellate shown comprise complex mixture epithelial fractions specific local enrichment phenotypes along microchannel. Following this importance passive, paracrine developmental formation. Using such crucial first step develop stem cell-derived vitro systems that compare functionally currently toxicological applications.

Language: Английский

Citations

Six-gene prognostic signature for non-alcoholic fatty liver disease susceptibility using machine learning DOI

Xiang Zhang,

Chunzi Zhou,

Jingwen Hu

et al.

Medicine, Journal Year: 2024, Volume and Issue: 103(19), P. e38076 - e38076

Published: May 10, 2024

Background: nonalcoholic fatty liver disease (NAFLD) is a common affecting the global population and its impact on human health will continue to increase. Genetic susceptibility an important factor influencing onset progression, there lack of reliable methods predict normal populations NAFLD using appropriate genes. Methods: RNA sequencing data relating was analyzed “limma” package within R software. Differentially expressed genes were obtained through preliminary intersection screening. Core by establishing comparing 4 machine learning models, then prediction model for constructed. The effectiveness evaluated, applicability reliability verified. Finally, we conducted further gene correlation analysis, analysis biological function immune infiltration. Results: By algorithms, identified SVM as optimal model, with first 6 (CD247, S100A9, CSF3R, DIP2C, OXCT 2 PRAMEF16) predictive nomogram found have good effectiveness. Six genes’ receiver operating characteristic curves (ROC) suggest essential role in pathogenesis, they exhibit high value. Further immunology demonstrated that these closely connected various cells pathways. Conclusion: This study has successfully constructed advanced based diagnostic markers NAFLD, while also providing insights potential targeted therapies.

Language: Английский

Citations

Biocompatible and nondegradable microcapsules using an ethylamine-bridged EGCG dimer for successful therapeutic cell transplantation DOI

Seonmi Jang, Jae Bin Lee, Chaerim Yoo

et al.

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 373, P. 520 - 532

Published: July 26, 2024

Language: Английский

Citations

A review of current state-of-the-art materiobiology and technological approaches for liver tissue engineering DOI

Shadil Ibrahim Wani, Tanveer Ahmad Mir, Makoto Nakamura

et al.

Bioprinting, Journal Year: 2024, Volume and Issue: 42, P. e00355 - e00355

Published: Aug. 24, 2024

Language: Английский

Citations

Effect of Hydrodynamic Shear Stress on Algal Cell Fate in 3D Extrusion Bioprinting DOI

Md Shihab Shakur, Emily Lazarus, Can Wang

et al.

Advanced Engineering Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 13, 2024

The 3D bioprinting of aquatic photosynthetic organisms holds potential for applications in biosensing, wastewater treatment, and biofuel production. While algae cells can be immobilized bioprinted cell‐friendly matrices, there is a knowledge gap regarding the thresholds hydrodynamic shear stress that affect cells’ functionality viability during bioprinting. This study examines effect on fate Chlamydomonas reinhardtii cells. Computational fluid dynamics models based Navier–Stokes equations are developed to numerically predict stresses experienced by extrusion. Parallelly, cell culture experiments conducted evaluate functionality, growth rates, within constructs. By correlating simulation results, causal link between nozzle function has been characterized. findings highlight significantly impacted process factors. Notably, more sensitive than viability. Functional impairments occur at maximum around 5 kPa, while remains unaffected. Beyond 14 both decline irreversibly. results emphasize importance assessing after bioprinting, rather just

Language: Английский

Citations

The Promise and Challenges of Bioprinting in Tissue Engineering DOI

Ryan Martin,

Daeha Joung

Micromachines, Journal Year: 2024, Volume and Issue: 15(12), P. 1529 - 1529

Published: Dec. 23, 2024

Organ transplantation, biomimetic organ models, and the restoration of damaged or eviscerated tissues have been key goals in surgical medical research since their inception [...]

Language: Английский

Citations