Development of a dry powder insufflation device with application in in vitro cell-based assays in the context of respiratory delivery DOI Creative Commons
Jorge F. Pontes, Hermı́nio P. Diogo, Eusébio Conceição

et al.

European Journal of Pharmaceutical Sciences, Journal Year: 2024, Volume and Issue: 197, P. 106775 - 106775

Published: April 20, 2024

Research on pharmaceutical dry powders has been increasing worldwide, along with increased therapeutic strategies for an application through the pulmonary or nasal routes. In vitro methodologies and tests that mimic respiratory environment process of inhalation itself are, thus, essential. The literature frequently reports cell-based in assays involve testing suspension. This experimental setting is not adequate, as both lung cavity are devoid abundant liquid. However, devices permit powder insufflation over cells culture either scarce technically complex expensive, which feasible early stages research. this context, work proposes development a device allows delivery onto cell surfaces, thus simulating more appropriately. Subsequently, quartz crystal microbalance (QCM) was used to establish technique enabling determination deposition profiles. Additionally, viability (A549) after using developed performed. all, prototype designed developed, 3D printing methods its production. It allowed homogenous dispersion insufflated petri dish QCM crystal, detailed study how disperse supports. device, already protected by patent, still requires further improvement, especially regarding method weighing efficiency process, being addressed. impact air locust bean gum (LBG)-based microparticles revealed absence cytotoxic effect, roughly above 70% always determined.

Language: Английский

Enhancing glioblastoma therapy via intranasal administration of highly potent cell-penetrating peptide decorated nanoparticles DOI

Jain Koo,

Yuseon Shin,

Hyewon Jeon

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 378, P. 997 - 1012

Published: Jan. 5, 2025

Language: Английский

Citations

1
Cancer cell membrane-decorated hybrid liposomes for treating metastatic breast cancer based on enhanced cancer immunotherapy DOI

Junyeong Lee,

Woo Tak Lee,

Xuan Thien Le

et al.

Journal of Pharmaceutical Investigation, Journal Year: 2024, Volume and Issue: 54(4), P. 453 - 465

Published: Jan. 31, 2024

Language: Английский

Citations

5
Engineering a self-healing grafted chitosan–sodium alginate based hydrogel with potential keratinocyte cell migration property and inhibitory effect against fluconazole resistance Candida albicans biofilm DOI
Vinit Raj, Chaitany Jayprakash Raorane,

Divya Shastri

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 261, P. 129774 - 129774

Published: Jan. 28, 2024

Language: Английский

Citations

4
A thermosensitive chitosan hydrogel: An attempt for the nasal delivery of dimethyl fumarate DOI Creative Commons

Noelia Nieto González,

Giovanna Rassu, Massimo Cossu

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 278, P. 134908 - 134908

Published: Aug. 23, 2024

Dimethyl fumarate (DMF) is a drug that orally administered for the treatment of relapsing-remitting multiple sclerosis. However, DMF causes gastrointestinal side effects and flushing in 43 % patients, which significantly contributes to discontinuation. To reduce increase patient compliance, aim this study was develop thermosensitive chitosan/glycerophosphate hydrogel nasal administration DMF. A binary system with hydroxypropyl-β-cyclodextrin (HP-β-CD) made included precursor solution. The solution (drug content, stability, thermogelling properties, viscosity), resulting (mucoadhesion, vitro permeation) were characterized. HP-β-CD able interact improve its water solubility. leader solution, G1 loaded approximately 92 DMF, remained stable 21 days. formed 2–1 min; it had pH 6.8 ± 0.06 caused no significant change osmolality simulated medium. showed good mucoadhesive properties released permeated controlled manner. As result, potential new approach exploit intranasal treating

Language: Английский

Citations

4
Development of a dry powder insufflation device with application in in vitro cell-based assays in the context of respiratory delivery DOI Creative Commons
Jorge F. Pontes, Hermı́nio P. Diogo, Eusébio Conceição

et al.

European Journal of Pharmaceutical Sciences, Journal Year: 2024, Volume and Issue: 197, P. 106775 - 106775

Published: April 20, 2024

Research on pharmaceutical dry powders has been increasing worldwide, along with increased therapeutic strategies for an application through the pulmonary or nasal routes. In vitro methodologies and tests that mimic respiratory environment process of inhalation itself are, thus, essential. The literature frequently reports cell-based in assays involve testing suspension. This experimental setting is not adequate, as both lung cavity are devoid abundant liquid. However, devices permit powder insufflation over cells culture either scarce technically complex expensive, which feasible early stages research. this context, work proposes development a device allows delivery onto cell surfaces, thus simulating more appropriately. Subsequently, quartz crystal microbalance (QCM) was used to establish technique enabling determination deposition profiles. Additionally, viability (A549) after using developed performed. all, prototype designed developed, 3D printing methods its production. It allowed homogenous dispersion insufflated petri dish QCM crystal, detailed study how disperse supports. device, already protected by patent, still requires further improvement, especially regarding method weighing efficiency process, being addressed. impact air locust bean gum (LBG)-based microparticles revealed absence cytotoxic effect, roughly above 70% always determined.

Language: Английский

Citations

1