Lighting the Way to Seizure Cessation: Transcranial Optogenetic Therapies to Stop Seizures in Mouse Models DOI Open Access
Gordon F. Buchanan

Epiliepsy currents/Epilepsy currents, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

Suppression of epileptic seizures by transcranial activation K+-selective channelrhodopsin Duan X, Zhang C, Wu Y, Ju J, Xu Z, Li Ohdah S, Constantin OM, Pan Lu Wang Chen Gee CE, Nagel G, Hou S-T, Gao and Song K. 2025. Nature Commun . 16(1):559. PMID: 39780018. Optogenetics is a valuable tool for studying the mechanisms neurological diseases now being developed therapeutic applications. In rodents macaques, improved channelrhodopsins have been applied to achieve optogenetic stimulation. While photoexcitation neurons has achieved, noninvasive inhibition treating hyperexcitability-induced disorders remained elusive. There critical need effective inhibitory tools that are highly light-sensitive capable suppressing neuronal activity in deep brain tissue. this study, we sensitive moderately K + -selective (HcKCR1-hs) molecular engineering recently discovered Hyphochytrium catenoides kalium (potassium) 1. Transcranial HcKCR1-hs significantly prolongs time first seizure, increases survival, decreases seizure several status epilepticus mouse models. Our approach neural hyperactivity may be adapted cell type-specific neuromodulation both basic preclinical settings.

Language: Английский

Effects of Polydatin on Pentylenetetrazol-Induced Seizures in Zebrafish Larvae DOI Creative Commons
Fernanda Barros de Miranda, Lúcia Emanueli Schimith,

Dennis Guilherme da Costa Silva

et al.

Future Pharmacology, Journal Year: 2025, Volume and Issue: 5(2), P. 22 - 22

Published: May 15, 2025

Background/Objectives: Epilepsy is a common neurological condition characterized by the occurrence of seizure. It affects around 50 million individuals worldwide, and despite large quantity anti-seizure medications available, 30% epileptic patients still suffer from seizures. Therefore, it necessary to find new therapeutic options. Interestingly, polydatin has shown promising effects on epilepsy treatment due its antioxidant anti-inflammatory properties. Thus, this study aimed evaluate (200, 300, 400 µM) pentylenetetrazol (PTZ)-induced seizure model in wild-type zebrafish (Danio rerio) larvae. Methods: Seizure-like behavior, cell death, reactive species (RS) production, lipid peroxidation were analyzed. Results: Pre-treatment with at 200 300 µM did not have significant impact behavior animals exposed PTZ. Diazepam decreased increased latency achieve each stage. Exposure PTZ swimming activity, effect was suppressed diazepam but polydatin. exposure RS which significantly attenuated DMSO. Cell death changed when compared experimental groups. Conclusions: Only positive control (diazepam) showed effects. we failed observe any using model. However, cannot rule out other models different protocols.

Language: Английский

Citations

0
Lighting the Way to Seizure Cessation: Transcranial Optogenetic Therapies to Stop Seizures in Mouse Models DOI Open Access
Gordon F. Buchanan

Epiliepsy currents/Epilepsy currents, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

Suppression of epileptic seizures by transcranial activation K+-selective channelrhodopsin Duan X, Zhang C, Wu Y, Ju J, Xu Z, Li Ohdah S, Constantin OM, Pan Lu Wang Chen Gee CE, Nagel G, Hou S-T, Gao and Song K. 2025. Nature Commun . 16(1):559. PMID: 39780018. Optogenetics is a valuable tool for studying the mechanisms neurological diseases now being developed therapeutic applications. In rodents macaques, improved channelrhodopsins have been applied to achieve optogenetic stimulation. While photoexcitation neurons has achieved, noninvasive inhibition treating hyperexcitability-induced disorders remained elusive. There critical need effective inhibitory tools that are highly light-sensitive capable suppressing neuronal activity in deep brain tissue. this study, we sensitive moderately K + -selective (HcKCR1-hs) molecular engineering recently discovered Hyphochytrium catenoides kalium (potassium) 1. Transcranial HcKCR1-hs significantly prolongs time first seizure, increases survival, decreases seizure several status epilepticus mouse models. Our approach neural hyperactivity may be adapted cell type-specific neuromodulation both basic preclinical settings.

Language: Английский

Citations

0