Effectiveness of a Cannabinoids Supplement on Sleep and Mood in Adults With Subthreshold Insomnia: A Randomized Double‐Blind Placebo‐Controlled Crossover Pilot Trial

Health Science Reports, Journal Year: 2025, Volume and Issue: 8(2)

Published: Feb. 1, 2025

Conduct a pilot randomized double-blind placebo-controlled crossover trial for adults with subthreshold insomnia symptoms to examine the effectiveness of cannabinoids supplement on sleep quality and health outcomes. Adults (N = 20, Mage 47.40) were either Cannabinoids Supplement (CS) or Placebo Condition (PC) 10 days. The CS was an oral soft gel that contained 3 mg Δ9-tetrahydrocannabinol, 6 cannabinol, cannabidiol, 90 proprietary food-grade terpene blend. Following 2-week washout, they completed alternate condition. following validated questionnaires collected at baseline each condition: Insomnia Severity Index, Pittsburgh Sleep Quality Bergen Scale, Profile Mood States (POMS), Perceived Stress Pain Questionnaire. Trait Anxiety Inventory, Flinders Daytime Fatigue, Health-related Life Scale. Clinical registry number ISRCTN 15022302. When compared PC, had significantly improved quality/efficiency, symptoms, health-related life, p < 0.05. Nonsignificant improvements PC found POMS mood subscales tension, anger, fatigue, depression, vigor, as well anxiety. Esteem subscale from Baseline Post PC. Both Vigor baseline. CS. No adverse events reported. This cannabinoid-based formulation well-tolerated may improve adults' quality/efficiency life. Larger controlled trials are encouraged longer-term effects this in variety populations environments.

Language: Английский

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Structure and Dynamics of Cannabinoid Binding to the GABA_A Receptor

Proteins Structure Function and Bioinformatics, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

ABSTRACT Research on medical cannabis is progressing, with several cannabinoids emerging as promising compounds for clinical use. The available evidence suggests that may modulate the glycine receptor (GlyR) and GABA A receptor, which are part of pentameric ligand‐gated ion channels (pLGICs) superfamily facilitate chemical communication in nervous system. In a previous study, we employed molecular dynamics (MD) simulations to elucidate GlyR/Δ 9 ‐tetrahydrocannabinol (THC) complex successfully identified representative binding mode. Given structural similarity between GlyR R, similar strategy investigate R‐cannabinoid interactions. We initially assessed mode THC R‐α1β2γ2 at equivalent site GlyR—that is, its two α‐subunits—as well impact this channel's dimensions. Our results indicate, first, modes R exhibit comparable characteristics and, second, function potentiator activity due significant opening channel pore. Additionally, aimed reduce overall computational cost associated exploring modes. To end, developed validated simplified model comprising single‐monomer system cannabinoid studies. This proved be accurate cost‐effective, accelerating silico screening process allowing study through docking MD simulations. Moreover, analysis different cannabigerol (CBG) cannabichromene (CBC) could act ligands unexplored avenues research.

Language: Английский

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Optimization and Evaluation of Cannabis-Based Magistral Formulations: A Path to Personalized Therapy

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(1), P. 73 - 73

Published: Jan. 9, 2025

The official implementation of pharmaceutical-grade cannabis raw materials for medicinal use has permitted doctors to prescribe and pharmacists prepare cannabis-based formulations. objective the pharmaceutical development manufacturing process optimization work was propose a suppository formulation containing doses 25 mg 50 tetra-hydrocannabinol (∆-9-THC) as an alternative existing inhalable or orally administered could be used rectal vaginal administration, thereby providing dosage control in treatment endometriosis other conditions involving pain. In this study, two substrates from suppositories with standardized Cannabis extractum normatum (CEX) were used: cocoa butter Witepsol® H15. long-term stability CEX investigated over period up 24 months. concentrations ∆-9-THC, cannabidiol (CBD), cannabinol (CBN) determined using HPLC method. Furthermore, water content extract, ethanol residue, microbiological purity determined. properties CEX-incorporated suppositories, namely uniformity, hardness, softening time, total deformation disintegration release profile CBD, CBN, evaluated order develop optimal preparation procedures pharmacists. Following 24-month study on CEX, no significant alterations component observed beyond specified requirements. hardness based H15 found longer higher, respectively, than those formulated butter. vitro studies demonstrated that prepared exhibited superior ∆-9-THC compared We suggest making prescription drugs pharmacy setting form medical marijuana will receive better drug by choosing substrate.

Language: Английский

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Preparation and characterization of a certified reference material of toxic elements in cannabis leaves

Analytical and Bioanalytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Abstract The quantification of harmful elements in cannabis is a relevant analytical task that requires metrological tools to ensure the reliability measurement results. This work reports preparation and characterization certified reference material (CRM) for toxic elements—arsenic (As), cadmium (Cd), lead (Pb)—in pulverized, lyophilized leaf tissue. To prepare CRM INM-040-1, portion dried ground vegetal was spiked with at mass fraction values close levels interest regulations this kind matrix: 0.34 mg/kg arsenic, cadmium, 0.66 lead. were quantified by inductively coupled plasma spectrometry (ICP-MS) combination graphite furnace atomic absorption spectroscopy (GF-AAS, lead) or hydride generation (HG-AAS, arsenic). calibration done gravimetric standard addition ICP-MS GF-AAS, while bracketing used HG-AAS. Furthermore, internal correction during measurements. methods validated demonstrate their fitness purpose. variables (particle size, drying treatment, spiking conditions) studied improve homogeneity CRM. relative uncertainties ranging from 4.2 6.9%. uncertainty contributions considered elements’ measurements, between-methods bias, (in)homogeneity production batch, (in)stability under transport storage conditions. new constitutes useful tool laboratories assessing harmlessness materials, promoting humans’ safety regulatory compliance within medicinal industry. Graphical

Language: Английский

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A 13-week oral toxicity and toxicokinetic study of cannabidiol in Sprague Dawley rats with a 4-week recovery period

Drug and Chemical Toxicology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 14

Published: May 13, 2025

Cannabidiol (CBD) is widely marketed as a health and wellness product. However, evidence for its effectiveness safety remains limited. This study assessed the toxicity toxicokinetic profile of CBD in Sprague Dawley rats over 13 weeks at low (5 mg/kg/day), mid (15 high (150 mg/kg/day) doses, followed by 4-week recovery period. Toxicokinetic analyses revealed no marked sex differences systemic exposure to or metabolite 6-OH-CBD; however, female had slightly higher metabolites 7-OH-CBD 7-COOH-CBD. Accumulation was observed following repeated oral administration CBD. No CBD-related effects on mortality, clinical observations, ophthalmoscopy were during study. Higher food consumption treated with dose group; this did not correlate statistically significant increase body weight. A fold serum alanine aminotransferase (∼1.4-fold 1.5-fold) group, which determined be reversible. Histopathological showed hepatocyte hypertrophy, but effect accompanied inflammatory changes other microscopic lesions resolved Hypertrophy pars distalis cells pituitary gland cortical cell vacuolation adrenal glands adaptive reversible The no-observed level considered lower than lowest tested adverse highest mg/kg/day).

Language: Английский

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