Optimized Effects of Fisetin and Hydroxychloroquine on ER Stress and Autophagy in Nonalcoholic Fatty Pancreas Disease in Mice DOI Creative Commons

Mahboobe Sattari,

Amin Karimpour, Maryam Akhavan Taheri

et al.

Journal of Diabetes Research, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Background: Fat accumulation in the pancreas, known as nonalcoholic fatty pancreatic disease (NAFPD), is associated with obesity and may lead to prediabetes Type 2 diabetes. Reducing endoplasmic reticulum stress enhancing autophagy could offer therapeutic benefits. This study examines effects of fisetin (FSN) hydroxychloroquine (HCQ) on NAFPD. Method: Forty‐eight Male C57BL/6 J mice were assigned a standard chow diet (SCD) or high‐fat (HFD) for 16 weeks. The HFD group was divided into five subgroups; each contains eight mice: HFD, + V (vehicle), FSN, HCQ, FSN HCQ. given daily at 80 mg/kg, HCQ injected IP 50 mg/kg twice weekly more 8 Insulin resistance assessed through OGTT HOMA‐IR. Histological analysis tissue conducted, protein mRNA levels molecules ER using PCR immunoblotting techniques. Result: significantly reduced weight gain, adipocyte accumulation, insulin caused by obese mice, combination two compounds producing even pronounced effects. Additionally, increased expression UPR markers ATF4 CHOP, response that further intensified In contrast, attenuated regulating GRP78 levels. Furthermore, resulted significant decrease LC3II/LC3I ratio an p62 due p‐AMPK Following treatment these alterations reversed, leading decreased mTOR such ATG5 Beclin1. Conclusion: Our reveals effectively combat HFD‐induced NAFPD, improving sensitivity addressing fat deposition linked metabolic syndrome. While cause stress, offers protective effects, supporting their combined use better outcomes.

Language: Английский

Prunin: An Emerging Anticancer Flavonoid DOI Open Access
Juie Nahushkumar Rana, Sohail Mumtaz

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2678 - 2678

Published: March 16, 2025

Despite the substantial advances in cancer therapies, developing safe and effective treatment methodologies is critical. Natural (plant-derived compounds), such as flavonoids, might be crucial a methodology without toxicity toward healthy tissues. Prunin flavonoid with potential to used biomedical applications. has yet undergo thorough scientific research, its precise molecular mechanisms of action remain largely unexplored. This review summarizes therapeutic prunin for first time, focusing on underlying an anticancer compound. gained significant attention due antioxidant, anti-inflammatory, effects. aims unlock how functions at level exert effects, primarily modulating key cellular pathways. Furthermore, we have discussed prunin’s adjunctive therapy conventional treatments, highlighting ability strengthen responses while decreasing drug resistance. Moreover, discussion probes into innovative delivery methods, particularly nanoformulations, that address bioavailability, solubility, stability limitations optimize application. By providing comprehensive analysis properties, this stimulate further exploration using agent, thereby progressing development targeted, selective, safe, methods.

Language: Английский

Citations

0
Optimized Effects of Fisetin and Hydroxychloroquine on ER Stress and Autophagy in Nonalcoholic Fatty Pancreas Disease in Mice DOI Creative Commons

Mahboobe Sattari,

Amin Karimpour, Maryam Akhavan Taheri

et al.

Journal of Diabetes Research, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Background: Fat accumulation in the pancreas, known as nonalcoholic fatty pancreatic disease (NAFPD), is associated with obesity and may lead to prediabetes Type 2 diabetes. Reducing endoplasmic reticulum stress enhancing autophagy could offer therapeutic benefits. This study examines effects of fisetin (FSN) hydroxychloroquine (HCQ) on NAFPD. Method: Forty‐eight Male C57BL/6 J mice were assigned a standard chow diet (SCD) or high‐fat (HFD) for 16 weeks. The HFD group was divided into five subgroups; each contains eight mice: HFD, + V (vehicle), FSN, HCQ, FSN HCQ. given daily at 80 mg/kg, HCQ injected IP 50 mg/kg twice weekly more 8 Insulin resistance assessed through OGTT HOMA‐IR. Histological analysis tissue conducted, protein mRNA levels molecules ER using PCR immunoblotting techniques. Result: significantly reduced weight gain, adipocyte accumulation, insulin caused by obese mice, combination two compounds producing even pronounced effects. Additionally, increased expression UPR markers ATF4 CHOP, response that further intensified In contrast, attenuated regulating GRP78 levels. Furthermore, resulted significant decrease LC3II/LC3I ratio an p62 due p‐AMPK Following treatment these alterations reversed, leading decreased mTOR such ATG5 Beclin1. Conclusion: Our reveals effectively combat HFD‐induced NAFPD, improving sensitivity addressing fat deposition linked metabolic syndrome. While cause stress, offers protective effects, supporting their combined use better outcomes.

Language: Английский

Citations

0