Chicken C4BPM facilitates replication of H5N1 and H9N2 Avian Influenza Viruses by inhibiting the production of type I interferons

Poultry Science, Journal Year: 2025, Volume and Issue: 104(3), P. 104868 - 104868

Published: Jan. 31, 2025

Avian influenza viruses (AIVs) are potential pandemic of global concern, posing a major threat to both the poultry industry and human health. Host factors play key role in replication AIVs, while function complement component 4 binding protein, membrane (C4BPM) this process is still unclear. This research reports that C4BPM promotes H5N1 H9N2 AIVs by inhibiting type I interferons (IFNs). infection up-regulate expression chicken embryo fibroblast cells (DF-1). To verify knockout (C4BPM-KO) stable overexpressing (C4BPM-overexpressing) DF-1 were generated using lentivirus-mediated CRISPR/Cas9 gene editing technology molecular cloning strategies. Replication promoted C4BPM-overexpressing with inhibited IFNs (IFN-α, IFN-β), protein kinase R (PKR), cholesterol 25-hydroxylase (CH25H), interferon regulatory factor (IRF) 3, mitochondrial antiviral signaling (MAVS) compared cells. However, C4BPM-KO increased above related anti-viral factors. Further results showed did not affect viral adsorption, while, promote entry AIVs. These expand range host regulating suggest promising target for agent.

Language: Английский

Insights into Medication-Induced Osteonecrosis of the Jaw Through the Application of Salivary Proteomics and Bioinformatics

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12405 - 12405

Published: Nov. 19, 2024

Long-term treatment with bisphosphonates is accompanied by an increased risk of medication-related osteonecrosis the jaw (MRONJ). Currently, no clinically useful biomarkers for predictive diagnosis MRONJ are available. To investigate potential key proteins involved in pathogenesis MRONJ, a proteomic LC-MS/MS analysis saliva was performed. Differentially expressed (DEPs) were analyzed using BiNGO, ClueGO, cytoHubba, MCODE, KEGG, and ReactomeFI software packages Cytoscape platforms. In total, 1545 DEPs identified, including 43 up- 11 down-regulated 1.5-fold cut-off value

Language: Английский