Leaf spectroscopy reveals drought response variation inFagus sylvaticasaplings from across the species’ range DOI Creative Commons

Dave Kurath,

Sofia J. van Moorsel,

Jolanda Klaver

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 24, 2024

Abstract The common European beech ( F. sylvatica ) is sensitive to prolonged droughts, and its natural distribution expected shift with climate change. To persist in novel environments, young trees rely on the capacity express diverse response phenotypes. Several methods exist study drought effects their adaptive mechanisms, but these are usually destructive, challenging scale large sample numbers needed investigate biological variation. We conducted a garden experiment outdoors, under controlled watering conditions, 180 potted two-year-old saplings from 16 provenances across species’ range, representing three distinct genetic clusters. Drought stress was simulated by interrupting irrigation. measured leaf reflectance of visible short-wave infrared electromagnetic radiation. determined drought-induced changes biochemical structural traits derived spectral indices model optical properties assessed intraspecific diversity. quantified pigmentation, water balance, nitrogen, lignin, epicuticular wax, mass per area drought-treated different clusters, revealing differences likely responses drought. Iberian Peninsula showed signatures greater resistance, i.e., least trait change treatment. Stomatal conductance soil moisture were used assess severity. demonstrate that high-resolution, broad-range spectroscopy an effective non-destructive tool individual can characterize functional variation among trees.

Language: Английский

Smaller conifers are more resilient to drought DOI Creative Commons
J. Julio Camarero, Manuel Pizarro, David S. Gernandt

et al.

Agricultural and Forest Meteorology, Journal Year: 2024, Volume and Issue: 350, P. 109993 - 109993

Published: April 8, 2024

Warmer and drier climate conditions are negatively impacting conifers, which show different mechanisms to withstand drought stress including growth resilience. However, it is unclear traits make trees shrubs more resilient in terms of recovery. We addressed this question by measuring collecting from the literature several functional 19 conifers nine genera (Abies, Pinus, Pseudotsuga, Cedrus, Picea, Juniperus, Cupressus, Tetraclinis Taxus) three families (Pinaceae, Cupressaceae, Taxaceae) inhabiting sites contrasting Spain. These were correlated with resilience indices derived ring-width data (Rt, resistance; Rc, recovery; Rs, resilience) without accounting for their phylogenetic signal. Growth resistance (Rt) was related plant height (r = -0.52, p 0.04). (Rs) -0.93, < 0.001) leaf life span -0.51, 0.03), but positively thickness-to-span ratio 0.80, 0.001). relationship between Rs not significant (p 0.155) after signals. Lastly, recovery responsiveness climate, assessed as correlation water balance 0.66, 0.002). Height ratio, a surrogate xylem cavitation, linked post-drought subjected seasonal dry conditions.

Language: Английский

Citations

5
De novo transcriptome assembly and discovery of drought-responsive genes in white spruce (Picea glauca) DOI Creative Commons
Zoé Ribeyre, Claire Depardieu, Julien Prunier

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(1), P. e0316661 - e0316661

Published: Jan. 3, 2025

Forests face an escalating threat from the increasing frequency of extreme drought events driven by climate change. To address this challenge, it is crucial to understand how widely distributed species economic or ecological importance may respond stress. In study, we examined transcriptome white spruce ( Picea glauca (Moench) Voss) identify key genes and metabolic pathways involved in species’ response water We assembled a de novo transcriptome, performed differential gene expression analyses at four time points over 22 days during controlled stress experiment involving 2-year-old plants three genetically distinct clones, conducted enrichment analyses. The assembly analysis identified total 33,287 transcripts corresponding 18,934 annotated unique genes, including 4,425 that are uniquely responsive drought. Many had predicted functions associated with photosynthesis, cell wall organization, transport were down-regulated under conditions, while linked abscisic acid defense up-regulated. Our study highlights previously uncharacterized effect on lipid metabolism conifers significant changes several transcription factors, suggesting regulatory potentially acclimation. research represents fundamental step unraveling molecular mechanisms underlying short-term responses seedlings. addition, provides valuable source new genetic data could contribute selection strategies aimed enhancing resistance resilience changing climates.

Language: Английский

Citations

0
Leaf Spectroscopy Reveals Drought Response Variation in Fagus sylvatica Saplings From Across the Species' Range DOI Creative Commons

Dave Kurath,

Sofia J. van Moorsel,

Jolanda Klaver

et al.

Journal of Geophysical Research Biogeosciences, Journal Year: 2025, Volume and Issue: 130(2)

Published: Feb. 1, 2025

Abstract The common European beech ( Fagus sylvatica ), sensitive to prolonged drought, is expected shift its distribution with climate change. To persist in novel environments, young trees rely on the capacity express diverse response phenotypes. Several methods exist study drought effects and their adaptive mechanisms, but these are usually destructive challenging for large sample numbers needed investigate biological variation. We conducted a garden experiment outdoors, under controlled watering conditions, 180 potted 2‐year‐old saplings from 16 provenances across species' range, representing three distinct genetic clusters. Drought stress was simulated by interrupting irrigation stomatal conductance soil moisture were used assess severity. measured leaf reflectance of visible short‐wave infrared electromagnetic radiation determine drought‐induced changes biochemical structural traits derived spectral indices model optical properties. quantified pigmentation, water balance, nitrogen, lignin, epicuticular wax, mass per area drought‐treated saplings, revealing differences likely responses drought. F. Iberian Peninsula showed signatures greater resistance, that is, least change spectrally related pigments content. demonstrate high‐resolution spectroscopy an effective non‐destructive tool individual can characterize functional intraspecific variation among trees. Next, this approach should be scaled up canopy‐level or airborne support assessments forests.

Language: Английский

Citations

0
Remote sensing reveals inter‐ and intraspecific variation in riparian cottonwood (Populus spp) response to drought DOI Creative Commons
Megan Seeley, Benjamin C. Wiebe, Catherine A. Gehring

et al.

Journal of Ecology, Journal Year: 2025, Volume and Issue: unknown

Published: May 16, 2025

Abstract Understanding how vegetation responds to drought is fundamental for understanding the broader implications of climate change on foundation tree species that support high biodiversity. Leveraging remote sensing technology provides a unique vantage point explore these responses across and within species. We investigated interspecific two Populus ( P . fremontii , angustifolia ) their naturally occurring hybrids using leaf‐level visible through shortwave infrared (VSWIR; 400–2500 nm) reflectance. As F 1 backcross with either species, resulting in range genotypes, we heretofore refer collectively as ‘cross types’. additionally explored intraspecific variation P. response at leaf canopy levels reflectance data thermal unmanned aerial vehicle (UAV) imagery. employed several analyses assess genotype‐by‐environment (G × E) interactions concerning drought, including principal component analysis, vector machine spectral similarity index. Five key findings emerged: (1) Spectra all three cross types shifted significantly drought. The magnitude reaction norms can be ranked from hybrids> > suggesting differential drought; (2) Spectral space among constricted under indicating spectral—and phenotypic—convergence; (3) Experimentally, populations cool regions had different than warm regions, source population mean annual temperature driving direction VSWIR (4) UAV imagery revealed watered, warm‐adapted maintained lower temperatures retained more leaves cool‐adapted populations, but differences retention decreased when droughted. (5) These are consistent patterns local adaptation stress, demonstrating ability spectra detect ecological evolutionary function environments. Synthesis. Leaf‐level spectroscopy canopy‐level captured inter‐ water stress cottonwoods, which widely distributed arid This study demonstrates potential monitor predict impacts scales varying landscapes.

Language: Английский

Citations

0
Biological outliers: essential elements to understand the causes and consequences of reductions in maximum photochemical efficiency of PSII in plants DOI Creative Commons

Clara Julián,

Sabina Villadangos, Laia Jené

et al.

Planta, Journal Year: 2024, Volume and Issue: 260(1)

Published: June 19, 2024

Abstract Main conclusion By studying Cistus albidus shrubs in their natural habitat, we show that biological outliers can help us to understand the causes and consequences of maximum photochemical efficiency decreases plants, thus reinforcing importance integrating these often-neglected data into scientific practice. Outliers are individuals with exceptional traits often excluded analysis. However, this may result very important mistakes not accurately capturing true trajectory population, thereby limiting our understanding a given process. Here, studied role using semi-deciduous shrub C. growing Mediterranean-type ecosystem. We assessed interindividual variability winter, spring summer PSII population under Mediterranean conditions. A strong correlation was observed between ( F v / m ratio) leaf water desiccation. While did any damage at organ level during reductions ratio were associated mortality summer. all plants could recover after rainfalls, an increased organism level, despite extreme deficit temperatures exceeding 40ºC conclude that, once methodological excluded, only must be from analysis, but focusing on them is crucial plants.

Language: Английский

Citations

1
De novo transcriptome assembly and discovery of drought-responsive genes in eastern white spruce (Picea glauca) DOI Creative Commons
Zoé Ribeyre, Claire Depardieu, Julien Prunier

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 13, 2024

Abstract Background Forests face an escalating threat from the increasing frequency of extreme drought events driven by climate change. To address this challenge, it is crucial to understand how widely distributed species economic or ecological importance may respond stress. Here, we used RNA-sequencing investigate transcriptome responses at levels water stress in white spruce (Picea glauca (Moench) Voss), across North America. We began generating a assembly emphasizing short-term different developmental stages. also analyzed differential gene expression four time points over 22 days controlled experiment involving 2-year-old plants and three genetically unrelated clones. Results De novo analysis revealed total 33,287 transcripts (18,934 annotated unique genes), with 4,425 drought-responsive genes. Many that had predicted functions associated photosynthesis, cell wall organization, transport were down-regulated under conditions, while linked abscisic acid response defense up-regulated. Our study highlights previously uncharacterized effect on lipid metabolism genes conifers significant changes several transcription factors, suggesting regulatory potentially acclimation. Conclusion research represents fundamental step unraveling molecular mechanisms underlying seedlings. In addition, provides valuable source new genetic data could contribute selection strategies aimed enhancing resistance resilience changing climates.

Language: Английский

Citations

0
Multi-year drought strengthens positive and negative functional diversity effects on tree growth response DOI Creative Commons
Hernán Serrano‐León, Haben Blondeel,

Paula Glenz

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 21, 2024

Abstract Mixed-species forests are proposed as strategy to increase the resistance and resilience of drought stress. However, evidence suggest that increasing tree species richness does not consistently enhance growth responses drought. Moreover, diversity effects under unprecedented multiyear droughts remain uncertain, calling for a better understanding underlying processes. Here, we used network planted experiments investigate how drought-induced individual trees influenced by neighborhood functional traits focal species. We analyzed cores (948 across 16 species) from nine Europe featuring gradients (1–6 species), which experienced severe in recent years. Radial response was quantified tree-ring biomass increment using X-ray computed tomography. applied hydraulic trait-based models analyze single-year all sites site-specific during consecutive years six function diversity. The large variability partially explained species’ safety margin (representing tolerance) intensity, but independent or trait on strengthened were site-specific, with contrasting direction (both positive negative). This indicated opposing pathways events, possibly resulting competitive release greater water consumption diverse mixtures. conclude may differ considerably Our study highlights need consider approaches (specifically, traits) scale processes understand multifaceted mixtures prolonged

Language: Английский

Citations

0
Leaf spectroscopy reveals drought response variation inFagus sylvaticasaplings from across the species’ range DOI Creative Commons

Dave Kurath,

Sofia J. van Moorsel,

Jolanda Klaver

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 24, 2024

Abstract The common European beech ( F. sylvatica ) is sensitive to prolonged droughts, and its natural distribution expected shift with climate change. To persist in novel environments, young trees rely on the capacity express diverse response phenotypes. Several methods exist study drought effects their adaptive mechanisms, but these are usually destructive, challenging scale large sample numbers needed investigate biological variation. We conducted a garden experiment outdoors, under controlled watering conditions, 180 potted two-year-old saplings from 16 provenances across species’ range, representing three distinct genetic clusters. Drought stress was simulated by interrupting irrigation. measured leaf reflectance of visible short-wave infrared electromagnetic radiation. determined drought-induced changes biochemical structural traits derived spectral indices model optical properties assessed intraspecific diversity. quantified pigmentation, water balance, nitrogen, lignin, epicuticular wax, mass per area drought-treated different clusters, revealing differences likely responses drought. Iberian Peninsula showed signatures greater resistance, i.e., least trait change treatment. Stomatal conductance soil moisture were used assess severity. demonstrate that high-resolution, broad-range spectroscopy an effective non-destructive tool individual can characterize functional variation among trees.

Language: Английский

Citations

0