Identification and amplification of candidate genes for virus defence in common ash (Fraxinus excelsior L.) DOI Creative Commons
Zoltán A. Köbölkuti, Marius Rehanek, Susanne von Bargen

et al.

Journal of Plant Diseases and Protection, Journal Year: 2024, Volume and Issue: 132(1)

Published: Dec. 16, 2024

Abstract Across various European countries, Fraxinus excelsior L. is facing a decline caused by Hymenoscyphus fraxineus . The underlying mechanisms of the disease are still insufficiently understood in detail. Observations suggest that, addition to main pathogen, viral infections may also contribute ash trees. We described homologues F. genome formerly annotated virus resistance genes model species and tested applicability primers on these gene fragments. Further, prediction cis-acting regulatory elements transcription factor binding sites was accomplished, determine exact location promoters genome. After annotation filtering low-complexity regions, for use with genomic DNA were designed sequences that showed high degree similarity equality length translated regions regions. From newly primers, those most favourable primer selection parameters selected eight infected—four symptomatic four asymptomatic—samples. amplified products analysed agarose gel electrophoresis. all producing one-band amplicons, 36 pairs amplifying translated, promoter selected. Our study first step SNP discovery marker development test. further could be useful individuals infections, providing route more thorough understanding dieback–virus interaction.

Language: Английский

Identification and amplification of candidate genes for virus defence in common ash (Fraxinus excelsior L.) DOI Creative Commons
Zoltán A. Köbölkuti, Marius Rehanek, Susanne von Bargen

et al.

Journal of Plant Diseases and Protection, Journal Year: 2024, Volume and Issue: 132(1)

Published: Dec. 16, 2024

Abstract Across various European countries, Fraxinus excelsior L. is facing a decline caused by Hymenoscyphus fraxineus . The underlying mechanisms of the disease are still insufficiently understood in detail. Observations suggest that, addition to main pathogen, viral infections may also contribute ash trees. We described homologues F. genome formerly annotated virus resistance genes model species and tested applicability primers on these gene fragments. Further, prediction cis-acting regulatory elements transcription factor binding sites was accomplished, determine exact location promoters genome. After annotation filtering low-complexity regions, for use with genomic DNA were designed sequences that showed high degree similarity equality length translated regions regions. From newly primers, those most favourable primer selection parameters selected eight infected—four symptomatic four asymptomatic—samples. amplified products analysed agarose gel electrophoresis. all producing one-band amplicons, 36 pairs amplifying translated, promoter selected. Our study first step SNP discovery marker development test. further could be useful individuals infections, providing route more thorough understanding dieback–virus interaction.

Language: Английский

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