Enhanced TaqMan qPCR Assay for Phytophthora cinnamomi Detection and Quantification

Forest Pathology, Journal Year: 2025, Volume and Issue: 55(2)

Published: March 12, 2025

ABSTRACT Phytophthora cinnamomi is one of the world's most invasive plant pathogens, requiring accurate detection in both and soil samples for effective disease management. This study compares existing qPCR assays with newly developed targeting internal transcribed spacer (ITS) gene improved detection. Our findings revealed that few current primers reliably distinguish P. from closely related species, even those did showed inadequate sensitivity. To address this, we designed novel a species‐specific probe ITS region, achieving consistent amplification limit 10 fg. The new assay successfully detected artificial symptomatic samples, ensuring rapid precise identification. optimised detects quantifies soil, providing superior sensitivity specificity over methods supporting more pathogen

Language: Английский

Similarities and Differences Among Factors Affecting Complex Declines of Quercus spp., Olea europea, and Actinidia chinensis

Horticulturae, Journal Year: 2025, Volume and Issue: 11(3), P. 325 - 325

Published: March 16, 2025

The decline of perennial plant species, including oak, olive, and kiwifruit, is a phenomenon currently observed in many areas the world. In this review, such species are chosen precisely because, despite differences their botany, native distribution, current utilization, they all affected by significant global or local declines. An analysis main common causes involved could be useful for better understanding phenomenon. Quercus impacted “Chronic Oak Decline” (COD), “Sudden (SOD), “Acute (AOD). Italy, olive groves severely damaged “Olive Quick Decline Syndrome”, whereas kiwifruit orchards struck “Kiwifruit Vine Syndrome” (KVDS). Among abiotic inciting stressors, drought, warmer temperatures, waterlogging, within climate change scenario, declines described herein as well dysbiosis. involvement some aggressive phytopathogens another feature these Oomycetes contribute to COD, SOD, KVDS; Xylella fastidiosa subsp. pauca Botryosphaeriaceae affect enterobacteria AOD, representing decisive contributing factors. These quite complex, comprehensive approach required dissect facets involved. A altered host–microbial community relationships can lead more tailored managing Maintaining tree resilience Earth remains primary goal achieve preserving both natural ecosystems profitable crops.

Language: Английский

Genome data for 25 isolates of the prevalent forest and nursery pathogen, Phytophthora cinnamomi, from New Zealand.

Data in Brief, Journal Year: 2025, Volume and Issue: unknown, P. 111655 - 111655

Published: May 1, 2025

Language: Английский