Deregulation in adult IgA vasculitis skin as the basis for the discovery of novel serum biomarkers DOI Creative Commons

Matija Bajželj,

Matjaž Hladnik,

Rok Blagus

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 12, 2023

Abstract Introduction Immunoglobulin A vasculitis (IgAV) in adults has a variable disease course, with patients often developing gastrointestinal and renal involvement thus contributing to higher mortality. Due understudied molecular mechanisms IgAV currently used biomarkers for visceral are largely lacking. Our aim was search potential serum based on the skin transcriptomic signature. Methods RNA sequencing analysis conducted biopsies collected from 6 treatment-naïve (3 only 3 involvement) healthy controls (HC) get insight into deregulated processes at level. 15 analytes were selected measured transcriptome (adiponectin, lipopolysaccharide binding protein (LBP), matrix metalloproteinase-1 (MMP1), C-C motif chemokine ligand (CCL) 19, kallikrein-5, CCL3, leptin, C-X-C (CXCL) 5, osteopontin, interleukin (IL)-15, CXCL10, angiopoietin-like 4 (ANGPTL4), SERPIN A12/vaspin, IL-18 fatty acid-binding (FABP4)) sera of 59 22 HC. Machine learning assess ability predict its organ involvement. Results Based gene expression levels skin, we able differentiate between HC using principal component (PCA) sample-to-sample distance matrix. Differential revealed 49 differentially expressed genes (DEGs) all patient’s vs Patients had more DEGs than (507 46 DEGs) as compared HC, suggesting different signatures. Major dysregulated lipid metabolism, acute inflammatory response, extracellular (ECM)-related processes. 11 affected (osteopontin, LBP, ANGPTL4, IL-15, FABP4, CCL19, MMP1) significantly (p-adj < 0.05) Prediction models utilizing showed high predicting adult IgAV. Conclusion Skin data deregulations metabolism reflected also analyte measurements. among others, could serve biomarker complications, while adiponectin CXCL10 indicate

Language: Английский

Deregulation in adult IgA vasculitis skin as the basis for the discovery of novel serum biomarkers DOI Creative Commons

Matija Bajželj,

Matjaž Hladnik,

Rok Blagus

et al.

Arthritis Research & Therapy, Journal Year: 2024, Volume and Issue: 26(1)

Published: April 12, 2024

Abstract Introduction Immunoglobulin A vasculitis (IgAV) in adults has a variable disease course, with patients often developing gastrointestinal and renal involvement thus contributing to higher mortality. Due understudied molecular mechanisms IgAV currently used biomarkers for visceral are largely lacking. Our aim was search potential serum based on the skin transcriptomic signature. Methods RNA sequencing analysis conducted biopsies collected from 6 treatment-naïve (3 only 3 involvement) healthy controls (HC) get insight into deregulated processes at level. 15 analytes were selected measured transcriptome (adiponectin, lipopolysaccharide binding protein (LBP), matrix metalloproteinase-1 (MMP1), C-C motif chemokine ligand (CCL) 19, kallikrein-5, CCL3, leptin, C-X-C (CXCL) 5, osteopontin, interleukin (IL)-15, CXCL10, angiopoietin-like 4 (ANGPTL4), SERPIN A12/vaspin, IL-18 fatty acid-binding (FABP4)) sera of 59 22 HC. Machine learning assess ability predict its organ involvement. Results Based gene expression levels skin, we able differentiate between HC using principal component (PCA) sample-to-sample distance matrix. Differential revealed 49 differentially expressed genes (DEGs) all patient’s vs. Patients had more DEGs than (507 46 DEGs) as compared HC, suggesting different signatures. Major dysregulated lipid metabolism, acute inflammatory response, extracellular (ECM)-related processes. 11 affected (osteopontin, LBP, ANGPTL4, IL-15, FABP4, CCL19, MMP1) significantly (p-adj < 0.05) Prediction models utilizing showed high predicting adult IgAV. Conclusion Skin data deregulations metabolism reflected also analyte measurements. among others, could serve biomarker complications, while adiponectin CXCL10 indicate

Language: Английский

Citations

4
The Role of CCL18 in Rheumatoid Arthritis Diseases DOI

Tan Yuebin

IgMin Research, Journal Year: 2025, Volume and Issue: 3(1), P. 021 - 026

Published: Jan. 1, 2025

Rheumatoid Arthritis (RA) is a chronic auto-immune disease that mostly occurs in joint space with remarkably painful swelling. RA detail pathologies remain unclear, but several C-C motif chemokines factors and Interleukin have been identified as associated correlated disease-associate activities (such CCL2, CCL18, IL-6). Among these factors, increasing evidence indicates CCL18 could be an appropriate drug target, it highly disease-associated (DAS28) (elevated level of both patient serum local synovial fluids). High-concentration fluids has reported to facilitate pro-inflammation factor production, finally resulting swelling space. In vivo studies showed significant efficiency anti-TNF-a treatment (CCl18 suppression). Most current approved drugs target TNF, IL-6, or other do not directly indirectly. From this perspective, believed new for therapeutic development.

Language: Английский

Citations

0
Lipocalin 2 inhibits the expressions of interleukin-8 and macrophage inflammatory protein-1α in human neutrophil-like cells DOI
Rie Kido, Yuka Hiroshima, Jun‐ichi Kido

et al.

Journal of Oral Biosciences, Journal Year: 2025, Volume and Issue: unknown, P. 100624 - 100624

Published: Feb. 1, 2025

Language: Английский

Citations

0
Staphylococcus Aureus Tames Nociceptive Neurons to Suppress Synovial Macrophage Responses for Sustained Infection in Septic Arthritis DOI Creative Commons
Xinyu Fang, Yang Chen,

Haiqi Ding

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Abstract The interaction between the nervous system and immune during chronic bacterial infection remains unclear. Here, it is reported that Staphylococcus aureus ( S. ) induces calcitonin gene‐related peptide (CGRP) secretion from intra‐articular transient receptor potential cation channel subfamily V member 1 positive (TRPV1 + nociceptive nerves through its pore‐forming toxin (PFT) α‐hemolysin. released CGRP then inhibits production of chemotactic cytokines by CX3CR1 tissue‐resident synovial lining macrophages via activity modifying protein (RAMP1) receptors at onset septic arthritis. During subsequent course infection, continuous release triggered pain has a lasting effect on antimicrobial capabilities macrophages, thereby promoting survival joint damage. This evidence suggests critical role for neuroimmune regulation in aureus‐ induced antagonism may reduce destruction, thus providing new option treating bone infections.

Language: Английский

Citations

0
Plasma CCL3 predicts adverse heart failure outcomes in patients with arrhythmogenic cardiomyopathy DOI Creative Commons
Hao Cui, Songren Shu, Ningning Zhang

et al.

BMC Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 13, 2025

Fibro-fatty replacement of the myocardium plays a key role in pathogenesis arrhythmogenic cardiomyopathy (ACM) and may be associated with progressive heart failure (HF). We aimed to investigate characteristic fibro-fatty tissues ACM patients plasma chemokines levels according HF burden. The expression level markers for brown, beige, white fat was determined using quantitative real-time polymerase chain reaction. Lipidomics analysis (n = 10 normal control [NC]; n 24 patients) conducted LC-MS. Single-cell RNA sequencing 2 NC; 6 used compare immune environment myocardium. Immunostaining enzyme-linked immunosorbent assay were examine CCL3 samples, respectively. beige (TBX1 TMEM26) brown (TNFRSF9) higher compared NC. revealed significant increased saturated triglycerides (TGs) obvious accumulation proinflammatory macrophages high positively correlated progression ACM. Plasma significantly healthy volunteer. A total 102 have been followed median 7.8 years, indicating that could successfully predict incidence transplantation (HTx)/death (hazard ratio 3.122 [95% confidence interval, 1.556-6.264]). ROC curve AUC value reached 0.814 0.756 HTx/death. TGs might promote patients. are useful predicting HF-related adverse events ACM, but requiring further validation larger independent cohorts.

Language: Английский

Citations

0
3,4-Dihydroxybenzoic acid methyl ester from Vespa velutina auraria Smith against rheumatoid arthritis via modulating the apoptosis and inflammation through NF-κB pathway DOI
Yi-Hao Che,

Chao-He Liu,

Xiu-Qin Pang

et al.

Inflammopharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: May 31, 2025

Language: Английский

Citations

0
Beyond CAR-T: The rise of CAR-NK cell therapy in asthma immunotherapy DOI Creative Commons

Mohadeseh Mohammad Taheri,

Fatemeh Javan,

Mohadeseh Poudineh

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Aug. 5, 2024

Asthma poses a major public health burden. While existing asthma drugs manage symptoms for many, some patients remain resistant. The lack of cure, especially severe asthma, compels exploration novel therapies. Cancer immunotherapy successes with CAR-T cells suggest its potential treatment. Researchers are exploring various approaches allergic diseases including membrane-bound IgE, IL-5, PD-L2, and CTLA-4 Dectin-1 fungal asthma. NK offer several advantages over T CAR-based immunotherapy. They key benefits: (1) HLA compatibility, meaning they can be used in wider range without the need matching tissue types. (2) Minimal side effects (CRS GVHD) due to their limited persistence cytokine profile. (3) Scalability "off-the-shelf" production from sources. Several strategies have been introduced that highlight superiority challenges CAR-NK cell therapy treatment IL-10, IFN-γ, ADCC, perforin-granzyme, FASL, KIR, NCRs (NKP46), DAP, DNAM-1, TGF-β, TNF-α, CCL, NKG2A, TF, EGFR. Furthermore, we advocate incorporating AI CAR design optimization CRISPR-Cas9 gene editing technology precise manipulation generate highly effective constructs. This review will delve into evolution designs, explore pre-clinical clinical studies therapies analyze optimize function, conduct comparative analysis respective challenges, finally present established designs promising

Language: Английский

Citations

3
Antiarthritic activity of Physalis peruviana fruit extract via inhibition of inflammatory mediators: Integrated in vitro, in vivo and in silico study DOI
Reda Sayed Mohammed, Faten M. Ibrahim, Radwa H. El‐Akad

et al.

Journal of Ethnopharmacology, Journal Year: 2023, Volume and Issue: 321, P. 117502 - 117502

Published: Nov. 27, 2023

Language: Английский

Citations

6
Gut microbiota was highly related to the immune status in chronic obstructive pulmonary disease patients DOI Creative Commons
Mei Wang, Jun Song,

Huizhen Yang

et al.

Aging, Journal Year: 2024, Volume and Issue: 16(4), P. 3241 - 3256

Published: Feb. 12, 2024

This study aimed to explore the profile of gut microbiota and immunological state in COPD patients. 80 fecal blood samples were collected from 40 patients healthy controls (HC) analyzed with 16s-rRNA gene sequencing immunofactor omics analysis investigate immunologic factors (IFs). The linear discriminant (LDA) effect size (LefSe) was used determine biomarker’s taxa. random forest LASSO regression executed screen IFs develop an IFscore model. correlation between IFs, along diversity microbiota, evaluated Spearman analysis. α β showed that composition distribution group differed HC group. 7 differential taxa at phylum level 17 genus found. LefSe screened out 5 32 (up-regulated 27 down-regulated IFs) identified two groups, (CCL3, CXCL9, CCL7, IL2, IL4) construct revealed 29 highly related enriched 16 pathways. Furthermore, relationship very close. individuals. Gut immune status

Language: Английский

Citations

2
Single-cell transcriptome analysis of macrophage subpopulations contributing to chemotherapy resistance in ovarian cancer DOI Creative Commons
Xiaolin Zhong, Fei Zhang,

Hongyang Xiao

et al.

Immunobiology, Journal Year: 2024, Volume and Issue: 229(5), P. 152811 - 152811

Published: May 18, 2024

Ovarian cancer, a fatal gynecological malignancy, is primarily managed through surgery and chemotherapy. However, significant challenge arises as patients frequently experience relapse due to chemotherapy resistance. This study delves into the complex functions underlying mechanisms of macrophages in resistance ovarian cancer.

Language: Английский

Citations

2