CRISPR-Cas13a-powered electrochemical biosensor for the detection of the L452R mutation in clinical samples of SARS-CoV-2 variants

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: April 29, 2023

Since the end of 2019, a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has deprived numerous lives worldwide, called COVID-19. Up to date, omicron is latest variant concern, and BA.5 replacing BA.2 become main subtype rampaging worldwide. These subtypes harbor an L452R mutation, which increases their transmissibility among vaccinated people. Current methods for identifying SARS-CoV-2 variants are mainly based on polymerase chain reaction (PCR) followed gene sequencing, making time-consuming processes expensive instrumentation indispensable. In this study, we developed rapid ultrasensitive electrochemical biosensor achieve goals high sensitivity, ability distinguishing variants, direct detection RNAs from viruses simultaneously. We used electrodes made MXene-AuNP (gold nanoparticle) composites improved sensitivity CRISPR/Cas13a system specificity in detecting single-base mutation clinical samples. Our will be excellent supplement RT-qPCR method enabling early diagnosis quick distinguishment Omicron more potential that might arise future.

Language: Английский

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Stimuli-Responsive Gene Delivery Nanocarriers for Cancer Therapy

Nano-Micro Letters, Journal Year: 2023, Volume and Issue: 15(1)

Published: Feb. 8, 2023

Gene therapy provides a promising approach in treating cancers with high efficacy and selectivity few adverse effects. Currently, the development of functional vectors safety effectiveness is intense focus for improving delivery nucleic acid drugs gene therapy. For this purpose, stimuli-responsive nanocarriers displayed strong potential overall efficiencies reducing effects via effective protection, prolonged blood circulation, specific tumor accumulation, controlled release profile drugs. Besides, synergistic could be achieved when combined other therapeutic regimens. This review summarizes recent advances various delivery. Particularly, responding to endogenous stimuli including pH, reactive oxygen species, glutathione, enzyme, etc., exogenous light, thermo, ultrasound, magnetic field, are introduced. Finally, future challenges prospects toward clinical translation well discussed. The major objective present biomedical cancer provide guidance developing novel nanoplatforms that clinically applicable.

Language: Английский

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CRISPR/Cas Systems‐Inspired Nano/Biosensors for Detecting Infectious Viruses and Pathogenic Bacteria

Small Methods, Journal Year: 2022, Volume and Issue: 6(10)

Published: Sept. 16, 2022

Abstract Infectious pathogens cause severe human illnesses and great deaths per year worldwide. Rapid, sensitive, accurate detection of is importance for preventing infectious diseases caused by optimizing medical healthcare systems. Inspired a microbial defense system (i.e., CRISPR/ CRISPR‐associated proteins (Cas) system, an adaptive immune protecting microorganisms from being attacked invading species), many new biosensors have been successfully developed widely applied in the viruses pathogenic bacteria. Moreover, advanced nanotechnologies also integrated into these to improve their stability, sensitivity, accuracy. In this review, recent advance CRISPR/Cas systems‐based nano/biosensors applications bacteria are comprehensively reviewed. First all, categories working principles systems establishing simply introduced. Then, design construction discussed. end, attentions focused on Impressively, remaining opportunities challenges further development system‐based promising proposed.

Language: Английский

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CRISPR-powered optothermal nanotweezers: Diverse bio-nanoparticle manipulation and single nucleotide identification

Light Science & Applications, Journal Year: 2023, Volume and Issue: 12(1)

Published: Nov. 16, 2023

Optothermal nanotweezers have emerged as an innovative optical manipulation technique in the past decade, which revolutionized classical by efficiently capturing a broader range of nanoparticles. However, optothermal temperature field was merely employed for in-situ nanoparticles, its potential identifying bio-nanoparticles remains largely untapped. Hence, based on synergistic effect and CRIPSR-based bio-detection, we developed CRISPR-powered (CRONT). Specifically, harnessing diffusiophoresis thermo-osmotic flows near substrate upon excitation, successfully trapped enriched DNA functionalized gold CRISPR-associated proteins, well strands. Remarkably, built scheme enhancing CRISPR-based single-nucleotide polymorphism (SNP) detection at single molecule level, while also introducing novel CRISPR methodology observing nucleotide cleavage. Therefore, this approach has endowed tweezers with identification ability aqueous solution unattainable before. With high specificity feasibility bio-nanoparticle identification, CRONT will become universal tool point-of-care diagnosis, biophotonics, bio-nanotechnology.

Language: Английский

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Recent Advances in CRISPR-Cas Technologies for Synthetic Biology

The Journal of Microbiology, Journal Year: 2023, Volume and Issue: 61(1), P. 13 - 36

Published: Jan. 1, 2023

Language: Английский

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CRISPR/Cas12a-Assisted isothermal amplification for rapid and specific diagnosis of respiratory virus on an microfluidic platform

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 237, P. 115523 - 115523

Published: July 9, 2023

Language: Английский

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A universal CRISPR/Cas12a-powered intelligent point-of-care testing platform for multiple small molecules in the healthcare, environment, and food

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 225, P. 115102 - 115102

Published: Jan. 25, 2023

Language: Английский

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CRISPR/Cas and Argonaute-Based Biosensors for Pathogen Detection

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(10), P. 3623 - 3642

Published: Oct. 11, 2023

Over the past few decades, pathogens have posed a threat to human security, and rapid identification of should be one ideal methods prevent major public health security outbreaks. Therefore, there is an urgent need for highly sensitive specific approaches identify quantify pathogens. Clustered Regularly Interspaced Short Palindromic Repeats CRISPR/Cas systems Argonaute (Ago) belong Microbial Defense Systems (MDS). The guided, programmable, targeted activation nucleases by both them leading way new generation detection. We compare these two in terms similarities differences. In addition, we discuss future challenges prospects development biosensors, especially electrochemical biosensors. This review expected afford researchers entering this multidisciplinary field useful guidance provide inspiration more innovative biosensors

Language: Английский

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Electrochemical nucleic acid sensors: Competent pathways for mobile molecular diagnostics

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 237, P. 115407 - 115407

Published: May 27, 2023

Language: Английский

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Non-canonical CRISPR/Cas12a-based technology: A novel horizon for biosensing in nucleic acid detection

Talanta, Journal Year: 2024, Volume and Issue: 271, P. 125663 - 125663

Published: Jan. 16, 2024

Language: Английский

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