Application of CRISPR-Cas System in HPV Detection Using Biosensor Devices and POC Technologies DOI Creative Commons
Chang He, Yongqi Li,

Jinkuan Liu

et al.

BME Frontiers, Journal Year: 2025, Volume and Issue: 6

Published: Jan. 1, 2025

Human papillomavirus (HPV) is the most common virus for genital tract infections. Cervical cancer ranks as fourth prevalent globally, with over 99% of cases in women attributed to HPV infection. This infection continues pose an ongoing threat public health. Therefore, development rapid, high-throughput, and sensitive detection platforms important, especially regions limited access advanced medical resources. CRISPR-based biosensors, a promising new method nucleic acid detection, are now rapidly widely used basic applied research have received much attention recent years diagnosis treatment. In this review, we discuss mechanisms functions CRISPR-Cas system, focusing on its applications diagnostics. The review covers CRISPR technologies such CRISPR-Cas9, CRISPR-Cas12, CRISPR-Cas13, along amplification methods, signal output systems, point-of-care testing (POCT) strategies. comprehensive overview highlights versatility potential detection. We also numerous biosensors developed since introduction detect HPV. Finally, some challenges faced by system.

Language: Английский

Development of a Single-Tube RPA/CRISPR-Cas12a Detection Platform for Monkeypox Virus DOI
Shan Liu, Yang Yang, Xue Li

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 278, P. 117221 - 117221

Published: Jan. 30, 2025

Language: Английский

Citations

0
Application of CRISPR-Cas System in HPV Detection Using Biosensor Devices and POC Technologies DOI Creative Commons
Chang He, Yongqi Li,

Jinkuan Liu

et al.

BME Frontiers, Journal Year: 2025, Volume and Issue: 6

Published: Jan. 1, 2025

Human papillomavirus (HPV) is the most common virus for genital tract infections. Cervical cancer ranks as fourth prevalent globally, with over 99% of cases in women attributed to HPV infection. This infection continues pose an ongoing threat public health. Therefore, development rapid, high-throughput, and sensitive detection platforms important, especially regions limited access advanced medical resources. CRISPR-based biosensors, a promising new method nucleic acid detection, are now rapidly widely used basic applied research have received much attention recent years diagnosis treatment. In this review, we discuss mechanisms functions CRISPR-Cas system, focusing on its applications diagnostics. The review covers CRISPR technologies such CRISPR-Cas9, CRISPR-Cas12, CRISPR-Cas13, along amplification methods, signal output systems, point-of-care testing (POCT) strategies. comprehensive overview highlights versatility potential detection. We also numerous biosensors developed since introduction detect HPV. Finally, some challenges faced by system.

Language: Английский

Citations

0