Synergistic effect of Hypoxic Conditioning and Cell‐Tethering Colloidal Gels enhanced Productivity of MSC Paracrine Factors and Accelerated Vessel Regeneration DOI Open Access
Myung Chul Lee, Jae Seo Lee, Seong-Soo Kim

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 9, 2024

Microporous hydrogels have been widely used for delivering therapeutic cells. However, several critical issues, such as the lack of control over harsh environment they are subjected to under pathological conditions and rapid egression cells from hydrogels, produced limited outcomes. To address these challenges, cell-tethering hypoxic conditioning colloidal containing mesenchymal stem (MSCs) introduced increase productivity paracrine factors locally in a long-term manner. Cell-tethering that composed tyramine-conjugated gelatin prevent egressing through on-cell oxidative phenolic crosslinks while providing mechanical stimulation interconnected microporous networks allow host-implant interactions. Oxygenating microparticles encapsulated microgels continuously generated oxygen 2 weeks with diffusion, resulting maintaining mild condition MSCs consumed severe hypoxia. Synergistically, local retention within hypoxic-conditioned mechanically robust significantly increased secretion various angiogenic cytokines chemokines. The oxygenating induced anti-inflammatory responses, reduced cellular apoptosis, promoted numerous large blood vessels vivo. Finally, mice injected MSC-tethered improved flow restoration muscle regeneration hindlimb ischemia (HLI) model.

Language: Английский

Advanced strategies in 3D bioprinting for vascular tissue engineering and disease modelling using smart bioinks DOI Creative Commons

Joeng Ju Kim,

Dong‐Woo Cho

Virtual and Physical Prototyping, Journal Year: 2024, Volume and Issue: 19(1)

Published: Aug. 30, 2024

Advanced three-dimensional (3D) bioprinting technology enables the precise production of complex vascular structures and biomimetic models, driving advancements in tissue engineering disease mechanism research. At core this is smart bioink, which suitable for fabricating models that can be vascularised to meet property requirements various tissues. Examples bioinks include decellularized extracellular matrix (dECM), photocrosslinkable, reversible, microgel-based biphasic (MB) bioinks, whose mechanical properties tuned through external stimuli. This tuning helps generate high-resolution complex-shaped networks essential cell survival functional maturation. review explores advanced 3D strategies using spatially controlled perfusable vitro emphasising reconstruction within bioprinted models. It also discusses challenges future prospects, suggesting could serve as alternatives traditional animal modelling drug screening.

Language: Английский

Citations

5
BDM editors pick the most memorable moments of 2024 DOI
Liang Ma, Jun Yin, Huayong Yang

et al.

Bio-Design and Manufacturing, Journal Year: 2025, Volume and Issue: 8(1), P. 166 - 168

Published: Jan. 1, 2025

Language: Английский

Citations

0
Bioxolography Using Diphenyliodonium Chloride and N‐Vinylpyrrolidone Enables Rapid High‐Resolution Volumetric 3D Printing of Spatially Encoded Living Matter DOI Creative Commons
Alexis Wolfel, Castro Johnbosco,

Annalise Anspach

et al.

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 26, 2025

Abstract Light‐based volumetric bioprinting enables fabrication of cubic centimeter‐sized living materials with micrometer resolution in minutes. Xolography is a light sheet‐based printing technology that offers unprecedented generation rates and print resolutions for hard plastics. However, the limited solubility reactivity current dual‐color photoinitiators (DCPIs) aqueous media have hindered their application high‐resolution matter. Here, we present novel three‐component formulation drastically improves photoreactivity thereby high‐resolution, rapid, cytocompatible Xolographic biofabrication intricately architected yet mechanically robust materials. To achieve this, various relevant additives are systematically explored, which revealed diphenyliodonium chloride N ‐vinylpyrrolidone strongly enhance D‐mediated photoreactivity, as confirmed by photo‐rheology. This gelatin methacryloyl‐based bioresins, producing >1 cm 3 constructs at ≈20 µm positive 125 negative within Multimaterial printing, molecular patterning, grayscale‐mediated mechanical patterning explored to programmably create intricate, biomimetic, concentration‐controlled architectures. We demonstrate Bioxolographic cell types, showing excellent viability, compatibility long‐term culture, ability nascent protein deposition. These results position Bioxolography transformative platform scalable, functional encoded chemical properties.

Language: Английский

Citations

0
Synergistic effect of Hypoxic Conditioning and Cell‐Tethering Colloidal Gels enhanced Productivity of MSC Paracrine Factors and Accelerated Vessel Regeneration DOI Open Access
Myung Chul Lee, Jae Seo Lee, Seong-Soo Kim

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 9, 2024

Microporous hydrogels have been widely used for delivering therapeutic cells. However, several critical issues, such as the lack of control over harsh environment they are subjected to under pathological conditions and rapid egression cells from hydrogels, produced limited outcomes. To address these challenges, cell-tethering hypoxic conditioning colloidal containing mesenchymal stem (MSCs) introduced increase productivity paracrine factors locally in a long-term manner. Cell-tethering that composed tyramine-conjugated gelatin prevent egressing through on-cell oxidative phenolic crosslinks while providing mechanical stimulation interconnected microporous networks allow host-implant interactions. Oxygenating microparticles encapsulated microgels continuously generated oxygen 2 weeks with diffusion, resulting maintaining mild condition MSCs consumed severe hypoxia. Synergistically, local retention within hypoxic-conditioned mechanically robust significantly increased secretion various angiogenic cytokines chemokines. The oxygenating induced anti-inflammatory responses, reduced cellular apoptosis, promoted numerous large blood vessels vivo. Finally, mice injected MSC-tethered improved flow restoration muscle regeneration hindlimb ischemia (HLI) model.

Language: Английский

Citations

1