The Promotion of Dark Septate Endophytes on the Performance and Active Ingredients Accumulation of Dendranthema morifolium Under Cd Stress

Agronomy, Journal Year: 2025, Volume and Issue: 15(1), P. 208 - 208

Published: Jan. 16, 2025

Dark septate endophytes (DSE) may facilitate plant growth and stress tolerance in stressful ecosystems. However, little is known about the response of medicinal plants to DSE, especially under heavy metal stress. This study aimed investigate how DSE affects Dendranthema morifolium cadmium (Cd) In this investigation, sterile non-sterile inoculations were carried out evaluate effect three strains on D. stressed with Cd. For root, DSE15 or inoculation resulted enhanced root biomass, volume, Cd content roots, indoleacetic acid (IAA) levels DSE7 significantly roots at 1 5 mg Cd/kg soil. Regarding impact stems leaves, conditions, effectively regulated shoot height, chlorophyll level, superoxide dismutase (SOD) content. Under positively influenced biomass while had no significant them when subjected effects flowers increased flower stress, reduced transfer coefficient Importantly, soil, promoted 1, 5-dicaffeoylquinic by 18.29% 21.70%. The interaction between soil factors revealed that species organic carbon available nitrogen content, These results contribute a better understanding DSE-plant interactions habitats contaminated metals demonstrate potential utility for cultivating plants.

Language: Английский

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Jet-Breaking Extrusion of Alginate–Chitosan Capsules for Encapsulation of Plant Growth–Promoting Extremophilic Fungi

Microorganisms, Journal Year: 2025, Volume and Issue: 13(5), P. 1123 - 1123

Published: May 14, 2025

Drought and metal pollution severely impact plant growth. Root-associated extremophilic fungi can improve performance, their encapsulation improves protection effectiveness. This study optimized the conditions for an fungus with growth-promoting traits using alginate–chitosan capsules. An endophytic was isolated from roots of Neltuma chilensis Atacama Desert identified via internal transcribed spacer (ITS) sequencing. Its traits, including exopolysaccharide, ammonium, siderophore, indole acetic acid production phosphorus solubilization, were evaluated. Freeze-dried Penicillium nalgiovense encapsulated jet-breaking extrusion, capsule morphology fungal survival assessed scanning electron microscope (SEM), confocal laser microscopy (CLSM), viability tests. Using Taguchi’s design, optimal sphericity (0.914 ± 0.002) mean size (3.232 0.087 mm) achieved 1% chitosan, a 5 cm distance to gelation bath, 40 Hz vibration frequency. CLSM analysis confirmed presence chitosan outer layer, revealing capsule’s coating material encapsulating P. nalgiovense. The remained viable across disinfection times, demonstrating effective gradual release. These findings emphasize need precise parameter control in encapsulation, providing basis developing robust bioinoculants support resilience extreme environments.

Language: Английский

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Evaluating Fungal Metal Tolerance Using MALDI‐TOF MS: A Rapid Alternative to Conventional MIC Methods

Journal of Basic Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: May 25, 2025

ABSTRACT Metal contamination represents a critical environmental challenge, adversely impacting ecosystems and human health. Microorganisms, including fungi, have developed diverse mechanisms to tolerate resist metal‐induced stress, making them valuables for bioremediation. This study evaluates the metal tolerance of Absidia glauca , Penicillium bilaiae Trichoderma viridescens using minimum inhibitory concentration (MIC) assay alternative profile change (MPCC) approach via MALDI‐TOF MS. MIC revealed species‐specific tolerances copper, zinc, cadmium. A. showed highest copper cadmium (75 9 mg L −1 ), producing dry biomass 0.03 0.04 g, respectively. While P. exhibited zinc ) 0.06 g. MS provided rapid proteomic information on fungal responses metals, showing changes in protein as increased. We performed comparative analysis between values obtained MPCC, giving positive correlation results both techniques Cu, Zn, Cd ( r = 1.00; 0.87 0.99 respectively, p < 0.05). In conclusion, has proven be an effective method analyzing exposure, providing more detailed molecular insights than traditional assays. Future studies should investigate underlying resistance, particularly focus regulation specific proteins.

Language: Английский

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Diversity and Functional Roles of Root-Associated Endophytic Fungi in Two Dominant Pioneer Trees Reclaimed from a Metal Mine Slag Heap in Southwest China

Microorganisms, Journal Year: 2024, Volume and Issue: 12(10), P. 2067 - 2067

Published: Oct. 15, 2024

The utilization of fast-growing, economically valuable woody plants with strong stress resistance, such as poplar and willow, to revegetate severely metal-contaminated mine tailings not only offers a productive profitable use abandoned polluted soil resources but also facilitates the phytoremediation these soils. This study examines diversity functional roles endophytic fungi naturally colonizing roots an artificially established

Language: Английский

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