Advances in RNA-Based Therapeutics: Challenges and Innovations in RNA Delivery Systems DOI Creative Commons
Yuxuan Liu, Yongkang Ou, Linlin Hou

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 47(1), P. 22 - 22

Published: Dec. 31, 2024

Nucleic acids, as carriers of genetic information, have found wide applications in both medical and research fields, including gene editing, disease diagnostics, drug development. Among various types nucleic RNA offers greater versatility compared to DNA due its single-stranded structure, ability directly encode proteins, high modifiability for targeted therapeutic regulatory applications. Despite promising potential biomedicine, RNA-based medicine still faces several challenges. Notably, one the most significant technical hurdles is achieving efficient delivery while minimizing immune responses. Various strategies been developed delivery, viral vectors, virus-like particles (VLPs), lipid nanoparticles (LNPs), extracellular vesicles (EVs). In this review, we explore these methods, highlight their advantages limitations, discuss recent advancements, providing insights future therapeutics.

Language: Английский

Integrated approaches to maximizing maize resistance to fall armyworm DOI Creative Commons
Sandra W. Woolfolk, Daniel Jeffers,

Leigh K. Hawkins

et al.

CABI Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

Abstract Fall armyworm (FAW) is a serious pest of maize causing major yield loss throughout the world. FAW management not easy primarily due to its ability migrate into areas lacking resistant cultivars, lack diapause, and evolving resistance control measures. Here, we discuss genetics both deployment genetic including historical breeding efforts, transgenic Bt traits, gene identification studies promote marker-assisted selection, various recent advancements accelerate improvement such as genome editing, genomic high throughput phenotyping, RNAi. can also be achieved using an integrated approach. Challenges IPM include climate change, complex cropping systems wide range hosts for FAW. We suggest that multinational cooperative public private research will needed create insect (IIRM) approach tying together crop, pest, predators; agronomic monitoring methods; changing environment. This give breeders time use advanced plant techniques deploy cultivars with stress resilience, stability grain quality.

Language: Английский

Citations

1

Identification of new HLA-A*0201-restricted cytotoxic T lymphocyte epitopes from LDHC in lung adenocarcinoma DOI Creative Commons
Ruifang Zhong, Xiaohong Guo,

Chuncai Wu

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 9, 2025

Lactate dehydrogenase C (LDHC) is a kind of cancer-testis antigen (CTA) that has been reported to be biomarker for diagnosis, efficacy evaluation, and recurrence monitoring lung adenocarcinoma (LUAD). This study aims assess the value LDHC in peptide-based vaccines LUAD immunotherapy. The recombinant protein was purified its effect on PC9 cells evaluated by wound healing assay, Transwell invasion, migration assay. Ten HLA-A2-restricted LDHC-derived peptides were predicted synthesized, affinity HLA-A2 molecule analyzed T2 binding assay docking. Enzyme-linked immunospot (ELISpot) LDH cytotoxicity performed determine interferon-γ (IFN-γ) release level tumor cell lysis ability peptide-induced specific cytotoxic T lymphocytes (CTLs). promoted invasion cells. Three P2 (LDHC170-180, FRYLIGEKLGV), P5 (LDHC116-124, IMKSIIPAI), P6 (LDHC172-180, YLIGEKLGV) had high at 50 μg/mL. elicited strongest IFN-γ-secreting lymphocyte (CTL) response exhibited potent against HLA-A2-positive with expression. may serve as targetable immunotherapy LUAD.

Language: Английский

Citations

0

Nanotechnology-Enhanced siRNA Delivery: Revolutionizing Cancer Therapy DOI

Donya Esmaeilpour,

Matineh Ghomi, Ehsan Nazarzadeh Zare‬

et al.

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: May 12, 2025

RNA interference (RNAi) has emerged as a transformative approach for cancer therapy, enabling precise gene silencing through small interfering (siRNA). However, the clinical application of siRNA-based treatments faces challenges such rapid degradation, inefficient cellular uptake, and immune system clearance. Nanotechnology-enhanced siRNA delivery revolutionized therapy by addressing these limitations, improving stability, tumor-specific targeting, therapeutic efficacy. Recent advancements in nanocarrier engineering have introduced innovative strategies to enhance safety precision therapies, offering new opportunities personalized medicine. This review highlights three key innovations nanotechnology-enhanced delivery: artificial intelligence (AI)-driven design, multifunctional nanoparticles combined strategies, biomimetic nanocarriers enhanced biocompatibility. AI-driven utilize machine learning algorithms optimize nanoparticle properties, drug release profiles minimizing off-target effects. Multifunctional integrate with chemotherapy, immunotherapy, or photothermal synergistic treatment approaches that outcomes reduce resistance. Biomimetic nanocarriers, including exosome-mimicking systems cell-membrane-coated nanoparticles, improve circulation time, evasion, targeted tumor delivery. These collectively precision, efficiency, therapies. The scope novelty lie their ability overcome primary barriers while paving way clinically viable solutions. provides comprehensive analysis latest developments fabrication, preclinical studies, assessments. By integrating multifunctionality, biomimicry, holds immense potential future therapy.

Language: Английский

Citations

0

Advances in RNA-Based Therapeutics: Challenges and Innovations in RNA Delivery Systems DOI Creative Commons
Yuxuan Liu, Yongkang Ou, Linlin Hou

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 47(1), P. 22 - 22

Published: Dec. 31, 2024

Nucleic acids, as carriers of genetic information, have found wide applications in both medical and research fields, including gene editing, disease diagnostics, drug development. Among various types nucleic RNA offers greater versatility compared to DNA due its single-stranded structure, ability directly encode proteins, high modifiability for targeted therapeutic regulatory applications. Despite promising potential biomedicine, RNA-based medicine still faces several challenges. Notably, one the most significant technical hurdles is achieving efficient delivery while minimizing immune responses. Various strategies been developed delivery, viral vectors, virus-like particles (VLPs), lipid nanoparticles (LNPs), extracellular vesicles (EVs). In this review, we explore these methods, highlight their advantages limitations, discuss recent advancements, providing insights future therapeutics.

Language: Английский

Citations

1