Construction and Validation of CRISPR/Cas Vectors for Editing the PDS Gene in Banana (Musa spp.) DOI Creative Commons
Marcelly Santana Mascarenhas, Fernanda dos Santos Nascimento, Luana Maria Pacheco Schittino

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(12), P. 14422 - 14437

Published: Dec. 20, 2024

Bananas and plantains are important staple food crops affected by biotic abiotic stresses. The gene editing technique via Clustered Regularly Interspaced Short Palindromic Repeats associated with the Cas protein (CRISPR/Cas) has been used as an tool for development of cultivars high tolerance to This study sought develop a protocol construction vectors knockout. Here we use phytoene desaturase (PDS) case in Prata-Anã banana nonhomologous end junction (NHEJ) method. PDS is key carotenoid production pathway plants its knockout leads easily visualized phenotypes such dwarfism albinism plants. Agrobacterium-mediated transformation delivered CRISPR/Cas9 constructs containing gRNAs were inserted into embryogenic cell suspension cultures. first provide effective method/protocol constructing vectors, demonstrating potential Brazilian variety. constitutive (CaMV 35S) root-specific successfully assembled confirmed transformed Agrobacterium DNA extraction PCR. specificity protocols makes it possible CRISPR-Cas9 enhanced tolerance/resistance major factors.

Language: Английский

CRISPR–Cas applications in agriculture and plant research DOI
Aytug Tuncel, Changtian Pan,

Joshua S. Clem

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Language: Английский

Citations

1
Rice Promoter Editing: An Efficient Genetic Improvement Strategy DOI Creative Commons
Bowen Wu,

Hangfei Luo,

Zhongbo Chen

et al.

Rice, Journal Year: 2024, Volume and Issue: 17(1)

Published: Aug. 30, 2024

Gene expression levels in rice (Oryza sativa L.) and other plant species are determined by the promoters, which directly control phenotypic characteristics. As essential components of genes, promoters regulate intensity, location, timing gene expression. They contain numerous regulatory elements serve as binding sites for proteins that modulate transcription, including transcription factors RNA polymerases. Genome editing can alter promoter sequences, thereby precisely modifying patterns specific ultimately affecting morphology, quality, resistance rice. This paper summarizes research on conducted recent years, focusing improvements yield, heading date, disease resistance. It is expected to inform application encourage further development crop genetic improvement with promote.

Language: Английский

Citations

3
Editing of upstream regulatory elements advances plant gene silencing DOI Open Access
Yuying Li, Pengcheng Wei

New Phytologist, Journal Year: 2024, Volume and Issue: 243(6), P. 2052 - 2054

Published: June 19, 2024

This article is a Commentary on Shen et al . (2024); 243 : 2501–2511

Language: Английский

Citations

0
Construction and Validation of CRISPR/Cas Vectors for Editing the PDS Gene in Banana (Musa spp.) DOI Creative Commons
Marcelly Santana Mascarenhas, Fernanda dos Santos Nascimento, Luana Maria Pacheco Schittino

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(12), P. 14422 - 14437

Published: Dec. 20, 2024

Bananas and plantains are important staple food crops affected by biotic abiotic stresses. The gene editing technique via Clustered Regularly Interspaced Short Palindromic Repeats associated with the Cas protein (CRISPR/Cas) has been used as an tool for development of cultivars high tolerance to This study sought develop a protocol construction vectors knockout. Here we use phytoene desaturase (PDS) case in Prata-Anã banana nonhomologous end junction (NHEJ) method. PDS is key carotenoid production pathway plants its knockout leads easily visualized phenotypes such dwarfism albinism plants. Agrobacterium-mediated transformation delivered CRISPR/Cas9 constructs containing gRNAs were inserted into embryogenic cell suspension cultures. first provide effective method/protocol constructing vectors, demonstrating potential Brazilian variety. constitutive (CaMV 35S) root-specific successfully assembled confirmed transformed Agrobacterium DNA extraction PCR. specificity protocols makes it possible CRISPR-Cas9 enhanced tolerance/resistance major factors.

Language: Английский

Citations

0