Beneficial Effects of N‐Acetyl Cysteine in the Different Equilibration Times on Post‐Thawed Rooster Sperm Quality

Reproduction in Domestic Animals, Journal Year: 2025, Volume and Issue: 60(3)

Published: March 1, 2025

Cryopreservation is the best method for preserving rooster sperm, especially in declining indigenous breeds. Cryopreserved semen significantly compromised due to equilibration time, cold shock and oxidative stress encountered during freezing-thawing process. To improve quality fertility of thawed semen, it essential protect sperm cells from peroxidative damage. This study assessed effects N-acetyl cysteine (NAC), an antioxidant supplement, on functional parameters after pre-freezing periods 2 4 h. Samples were collected 10 male Ross 308 broiler breeders diluted with Beltsville extenders containing different concentrations NAC (0, 0.1, 1 mM/mL) equilibrium h before freezing. Our findings showed that NAC-0.1 NAC-1 groups increased total progressive motility (59.85 ± 3.73, 59.67 42.85 2.64 42.80 2.64, respectively), viability, plasma membrane functionality (62.45 3.51, 62.36 56.81 3.51 56.82 3.56, respectively) compared control groups. Furthermore, NAC-0.1-2 NAC-1-2 demonstrated lowest levels apoptosis intracellular reactive oxygen species (ROS), as well highest mitochondrial potential comparison These indicate are effective maintaining a 2-h period.

Language: Английский

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Ellagic acid maintains post-thaw goat sperm quality via protecting mitochondrial function from ROS damage

Cryobiology, Journal Year: 2025, Volume and Issue: 119, P. 105231 - 105231

Published: March 24, 2025

Language: Английский

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Enhancing of Rabbit Sperm Cryopreservation with Antioxidants Mito-Tempo and Berberine

Antioxidants, Journal Year: 2024, Volume and Issue: 13(11), P. 1360 - 1360

Published: Nov. 6, 2024

Cryopreservation plays a critical role in animal breeding and the conservation of endangered species, but it often compromises sperm characteristics such as morphology, motility, viability due to oxidative stress. This study explores antioxidative effect Mito-Tempo (MT) Berberine (BER) enhance post-thaw quality rabbits. Pooled rabbit samples were supplemented with different concentrations (0.0, 0.5, 5, 10, 50 µmol/L) MT BER. Sperm motility was evaluated using computer-assisted semen analysis, while viability, apoptosis, reactive oxygen species (ROS) levels, acrosome integrity, mitochondrial function assessed through flow cytometry. The results revealed that at 5 10 µmol/L BER significantly improved total progressive activity, compared control group. Furthermore, enhanced both effectively reduced ROS levels apoptosis. is first demonstrate protective effects on during cryopreservation. By mitigating stress reducing these antioxidants markedly quality, positioning promising agents for improving cryosurvival.

Language: Английский

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Effects of different concentrations of N-acetylcysteine on the sperm quality, antioxidant enzyme activity, and antioxidant gene expression of cryopreserved goat semen

Theriogenology, Journal Year: 2024, Volume and Issue: 234, P. 101 - 109

Published: Dec. 10, 2024

Language: Английский

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Curcumin–loaded niosomal nanocarriers offer a promising approach to improve quality characteristics, apoptotic gene expression, and flow cytometry assessments of stallion spermatozoa after thawing

Cryobiology, Journal Year: 2024, Volume and Issue: 118, P. 105188 - 105188

Published: Dec. 29, 2024

Language: Английский

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Flow cytometry evaluation of Dalagh ram post-thaw semen incorporated with curcumin-loaded niosomal nanocarriers

Small Ruminant Research, Journal Year: 2024, Volume and Issue: 240, P. 107346 - 107346

Published: Aug. 28, 2024

Language: Английский

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Sulforaphane acts through the NFE2L2/AMPK signaling pathway to protect boar spermatozoa from cryoinjury by activating antioxidant defenses

Theriogenology, Journal Year: 2024, Volume and Issue: 230, P. 330 - 340

Published: Sept. 27, 2024

Language: Английский

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Niosomal nanocarriers loaded with artemisinin has potential to enhance the functional characteristics of cryo-preserved equine spermatozoa

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 272, P. 107653 - 107653

Published: Nov. 26, 2024

Language: Английский

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