Advanced Sperm Selection Techniques for Assisted Reproduction

Journal of Personalized Medicine, Journal Year: 2024, Volume and Issue: 14(7), P. 726 - 726

Published: July 4, 2024

Male infertility accounts for approximately 40% of cases. There are many causes male infertility, including environmental factors, age, lifestyle, infections, varicocele, and cancerous pathologies. Severe oligozoospermia, cryptozoospermia, azoospermia (obstructive non-obstructive) identified as severe factor once considered conditions sterility. Today, in vitro fertilization (IVF) techniques the only treatment strategy cases which new methodologies have been developed manipulation spermatozoa to achieve increase success rates. This review is an update techniques, particular sperm selection, emphasizing clinical case-specific methodology. The IVF process related diagnosis, appropriate choice treatment, effective preparation selection. In fact, selecting best guarantee optimal paternal heritage means increasing blastulation, implantation, ongoing pregnancy live birth rates, resulting greater techniques.

Language: Английский

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A Comparative Analysis of Gradient Centrifugation and Microfluidic Chips for Sperm Selection

IntechOpen eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 4, 2025

A variety of techniques have been employed in the field sperm selection, including methods based on density, morphological characteristics, motility membrane integrity, and surface charge. Among these techniques, density gradient centrifugation (DGC) method stands out as most prevalent widely utilized today. However, this technique involves steps that linked to oxidative stress subsequent cellular damage. Consequently, alternatives DGC are being continuously developed. The recently developed microfluidic chip has also implemented for selection intracytoplasmic injection (ICSI). presents certain disadvantages must be addressed. This chapter evaluates advantages from past present, with a particular focus devices.

Language: Английский

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Bioengineering Approaches for Male Infertility: From Microenvironmental Regeneration to in vitro Fertilization

Advances in experimental medicine and biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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Sperm Separation and Selection Techniques to Mitigate Sperm DNA Damage

Life, Journal Year: 2025, Volume and Issue: 15(2), P. 302 - 302

Published: Feb. 14, 2025

Semen preparation and sperm selection techniques exploit the morphological physiological characteristics of function, including motility, morphology, density, maturity, as reflected by their cell-surface charge expression hyaluronan receptors. The various methods employed have a common purpose mimicing within female reproductive tract and, thereby, increasing likelihood that oocytes will be fertilised spermatozoa with intact nuclear DNA normal genome. Indeed, paternal genome is relevant to embryonic activation blastocyst development, has fundamental impact upon successful implantation, ongoing pregnancy live birth. clinical use both well-established some more recently developed discussed in this comparative review separation from seminal plasma for insemination.

Language: Английский

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A Narrative Review on the Sperm Selection Methods in Assisted Reproductive Technology: Out with the New, the Old Is Better?

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(4), P. 1066 - 1066

Published: Feb. 7, 2025

Background: Male infertility, accounting for nearly half of infertility cases worldwide, has spurred significant research into its causes, diagnosis, and treatment strategies. Genetic abnormalities, social environmental exposures, lifestyle, further health conditions are key contributors. Methods: Essential to improving the outcomes ART is, among other things, selection high-quality sperm, which requires methods that assess sperm motility, morphology, DNA integrity, oxidative stress levels. Results: Traditional techniques such as semen analysis, swim-up, density gradient centrifugation (DGC) still widely used, but there is ongoing discussion regarding limitations in detecting damage stress. Advanced like magnetic-activated cell sorting (MACS) microfluidic have emerged more precise tools selecting with better genetic although they face challenges terms their standardization, cost, clinical adoption. Emerging technologies artificial intelligence (AI) Raman spectroscopy offer potential automated, accurate selection, minimizing human error variability. However, integration these practice validation through large-scale studies, including assessments long-term safety cost-effectiveness. Conclusions: Future should focus on refining techniques, tailoring them personalized approaches, addressing gaps evidence improve patient care.

Language: Английский

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Comparison of ICSI Outcomes Between Microfluidic and Conventional Sperm Selection Methods: A Systematic Review and Meta‐Analysis

Andrologia, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

In recent years, intracytoplasmic sperm injection (ICSI) has become the main selected option in more than half of assisted reproductive technologies (ARTs) cycles conducted each year. Selecting most competent cells with highest fertilization potential is crucial to improve clinical outcomes following ICSI procedure. Microfluidic (MF) chips have emerged as a new alternative conventional methods for selecting high‐quality spermatozoa minimal DNA fragmentation. However, due lack consensus about effects MF selection on outcomes, present study, we systematically reviewed and meta‐analyzed impact rate (FR), embryo quality (EQ), euploidy (ER), pregnancy (PR), live birth (LBR) ART procedures. A systematic comprehensive literature search PubMed, Scopus, Web Science databases was determine fertility compared methods. Meta‐analysis using random model used estimate pooled odds ratios (ORs) weighted mean differences (WMDs) various outcome measures. The identified 1714 records, which 21 articles were included review. significantly increased (OR = 1.04; 95% CI: 1.00–1.07; WMD 7.24, 6.93, 7.91) EQ 1.44; 1.30–1.60), while nonsignificantly increasing ER 1.20; 0.92–1.58), PR 1.09; 0.94–1.25), LBR 1.27; 0.99–1.62). Moreover, subgroup analyses revealed that resulted higher FR improved unexplained infertile male factor infertility patients. These results indicate couples history or may benefit from MF–based selection.

Language: Английский

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Development of a Thermotaxis and Rheotaxis Microfluidic Device for Motile Spermatozoa Sorting

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 258, P. 116353 - 116353

Published: April 30, 2024

Language: Английский

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