Evaluation of male contraceptive efficacy of Caesalpinia pulcherrima (L.) sw. in human and rat: an ex-vivo study

Systems Biology in Reproductive Medicine, Journal Year: 2025, Volume and Issue: 71(1), P. 43 - 53

Published: Feb. 19, 2025

The study focused on the spermicidal and anti-androgenic effects of aqueous-ethanolic (60:40) extract Caesalpinia pulcherrima leaves (AEECPL) in human rat samples from viewpoint its contraceptive efficacy through ex-vivo study. Six fertile adult males were selected randomly for semen collection. Parallelly sperm collected by epididymal washing six rats. Testes, epididymis, liver dissected Biological divided into control, 1, 2, 4 mg/ml AEECPL exposed groups. Relevant spermiological, steroidogenic enzymes, oxidative stress, metabolic toxicity sensors evaluated. All spermiological decreased significantly dose duration-dependent manners, number comet positive spermatozoa increased dose-dependent mode groups against control both rat. Activities Δ5,3β-hydroxysteroid dehydrogenase (HSD), 17β-HSD testis, kinetics superoxide dismutase testis epididymis along with elevation level thiobarbituric acid reactive substances glutamate oxaloacetate transaminase, pyruvate phosphatase, alkaline phosphatase above mentioned tissues showed no significant difference among groups, indicating non-toxic reproductive tissues. results presenting prominent preventing potentiality said optimal effect was noted at 2 dose. In-depth investigations are required in-vivo studies animal model to know genomic action execution male activity.

Language: Английский

Guhan Yangsheng Jing Mitigates Oxidative Stress and Ferroptosis to Improve Reproductive Damage in Diabetic Male Rats

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119746 - 119746

Published: April 1, 2025

Language: Английский