Characterizing the Ultraviolet (UV) Screening Ability of L-5-Sulfanylhistidine Derivatives on Human Dermal Fibroblasts

Marine Drugs, Journal Year: 2025, Volume and Issue: 23(2), P. 57 - 57

Published: Jan. 24, 2025

Using sunscreens is one of the most widespread measures to protect human skin from sun ultraviolet radiation (UVR) damage. However, several studies have highlighted toxicity certain inorganic and organic UV filters used in for marine environment health. An alternative strategy may involve use natural products origin counteract UVR-mediated Ovothiols are sulfur-containing amino acids produced by invertebrates, microalgae, bacteria, endowed with unique antioxidant UV-absorption properties. This study aimed evaluate protective effect synthetic L-5-sulfanyl histidine derivatives, inspired ovothiols, on dermal fibroblasts (HDFs) upon UVA exposure. By using a custom-made experimental set-up assess screening ability, we measured levels cytosolic mitochondrial reactive oxygen species (ROS), as well cell viability apoptosis HDFs, presence tested compounds, after exposure, flow cytometry assays specific fluorescent probes. The results show that derivatives display capacity prevent loss viability, production ROS induced exposure subsequent apoptosis. Overall, this sheds light potential applications marine-inspired developing eco-safe UVR protection.

Language: Английский

Integrating Transcriptomics and Metabolomics to Comprehensively Analyze Phytohormone Regulatory Mechanisms in Rhododendron chrysanthum Pall. Under UV-B Radiation

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1545 - 1545

Published: Feb. 12, 2025

In order to fully elucidate the roles and systems of phytohormones in UV-B radiation (UV-B) leaves Rhododendron chrysanthum Pall. (R. chrysanthum), we conducted a comprehensive analysis how R. protects itself against using transcriptomic metabolomic data. Transcript metabolite profiles were generated by combination deep sequencing LC-MS/MS (liquid chromatography-tandem mass spectrometry), respectively. Combined with physiological biochemical assays, studied compound accumulation, biosynthesis expression signaling genes seven hormones effects on plant photosynthesis. The findings indicate that during leaf defense UV-B, photosynthesis declined, photosynthetic system was impaired concentration salicylic acid (SA) increased, whereas contents cytokinin (CK), abscisic (ABA), ethylene, auxin, jasmonic (JA) gibberellins (GAs) continued decrease. Finally, correlation tests between hormone content analyzed, closely related resistance identified pathways. These results will expand our understanding hormonal regulatory mechanisms at same time lay foundation for adversity stress.

Language: Английский

Interactive effects of elevated atmospheric CO2 and UV-B radiation: A multi-level study on marine diatom Skeletonema pseudocostatum

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 291, P. 117879 - 117879

Published: Feb. 1, 2025

Climate change as a result of increases in greenhouse gas emissions, such CO2, is causing significant alteration global environmental conditions, including ocean acidification (OA). Although the depletion ozone layer has reduced, penetration ultraviolet-B (UVB) radiation into oceans still remains an factor that may potentially enhance effects OA on biota. Improved understanding complex interactions between multiple stressors, UV-B and increased CO2 levels, thus important for safeguarding ecosystems developing effective conservation management strategies. A 72 h experiment was carried out to investigate combined UVB irradiance (0.5 W m-2) varying levels (350, 500, 1000 ppm) diatom Skeletonema pseudocostatum. The study aimed characterize potential at different biological organization, ROS formation, lipid peroxidation (LPO), photosynthesis, pigments, oxidative phosphorylation (OXPHOS) growth. findings indicate exposure elevated (500 alone resulted total carotenoid content growth S. pseudocostatum, but did not significantly impact photosystem efficiency, stress, OXPHOS. Sole induced inhibited photosynthesis OXPHOS processes, suppressed However, when co-exposed with synergistic impacts were observed reactive oxygen species (ROS), growth, while carotenoids reduced antagonistic manner. putative pathway proposed initial effort these stressors under future marine scenarios involving CO2.

Language: Английский