Photoregulation of the biosynthetic activity of fungus Inonotus obliquus using colloidal solutions of biogenic metal nanoparticles and low-intensity laser radiation

Bioengineered, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 28, 2025

This article presents new data on the integrated use of colloidal solutions nanoparticles and low-intensity laser radiation biosynthetic activity medicinal mushroom Inonotus obliquus in vitro. Traditional mycological methods, biogenic metals, unique photobiological methods have also been used. It was found that all metals used increased growth characteristics I. (55-60%), while irradiation fungal inoculum with light a medium reduced mycelia by 12.3-35.4%. Silver (AgNPs) nutrient suppressed biosynthesis extracellular polysaccharides, whereas same synthesis intracellular polysaccharides 9.7 times. Magnesium (MgNPs) iron (FeNPs) inhibited mycelial mass obliquus. At time, MgNPs, contrary, induced sharp increase amount culture liquid (20 times). Treatment caused an intensification flavonoids melanin pigments (25-140%). The results obtained suggest possibility complex Fe, Ag, Mg as environmentally friendly factors for regulating biotechnology cultivating valuable

Language: Английский

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Influence of low-intensity artificial light on the fatty acid profile of the biotechnologically important culinary mushroom Pleurotus eryngii in vitro

BMC Biotechnology, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 24, 2025

The problem of searching for environmentally friendly regulators the biosynthetic activity edible and medicinal mushrooms is crucial creating highly effective biotechnologies. One such regulator light. This study aimed to compare evaluate fatty acid profile fat quality indices lipids from mycelial mass Pleurotus eryngii under various light-emitting diode (LED) laser light irradiation regimes. To determine effect artificial on P. eryngii, an lighting system based LED matrices with wavelengths 470 nm (blue), 530 (green), 650 (red), as well argon gas a coherent visible source at 488 nm, was used. For all experimental variants, energy density surface samples set same, providing dose 240 mJ/cm2. Irradiation carried continuously. Twenty-seven acids were identified in studied samples, including nine saturated (SFAs), eight monounsaturated (MUFAs) ten polyunsaturated (PUFAs). control sample (without irradiation) contained lowest number (fourteen). With modes, decrease amount SFA formation new MUFA PUFA chain length C20-C24, which absent control, observed. Blue stimulated synthesis significant amounts α-linolenic (C18:3ω-3). ratios ΣPUFA/ΣSFA, ΣPUFA/ΣMUFA ΣPUFAω-6/ω-3 mycelium irradiated blue within optimal values human diet. selected photoactivation modes using low-intensity different spectral composition coherence may have potential biotechnology submerged cultivation obtain improved profile, can be considered useful lipids.

Language: Английский

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PHOTOREGULATION OF THE BIOSYNTHETIC ACTIVITY OF LARICIFOMES OFFICINALIS USING COLLOIDAL SOLUTIONS OF METAL NANOPARTICLES AND LASER IRRADIATION

Biotechnologia Acta, Journal Year: 2024, Volume and Issue: 17(3), P. 66 - 77

Published: June 28, 2024

The aim of the work was to study influence biogenic metal nanoparticles on growth characteristics and biosynthetic activity fungus Laricifomes officinalis, as well effects photocatalytic NPs after exposure low-intensity laser radiation under deep cultivation conditions. Material Methods. Traditional mycological methods, colloidal solutions metals, unique photobiological methods were used. Results. Treatment inoculum with all used metals ncreased L. officinalis by 31–54%, while irradiation fungal light in a medium reduced mycelium 14.4–22.6%. All suppressed biosynthesis extracellular polysaccharides, whereas treatment FeNPs MgNPs stimulated synthesis endopolysaccharides. At same time, presence AgNPs increased amount endopolysaccharides, slightly inhibited their synthesis. affected total phenolic content (TPC) mycelial mass. highest TPC values ethanol extracts 97.31Ѓ}3.7 mg GAEs/g dry Conclusions. research results gave ground consider (AgNPs, FeNPs, MgNPs) promising regulators biotechnology its cultivation.

Language: Английский

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Effects of Blue Light on Fruiting Body Production and Ergothioneine Content During Sawdust Cultivation of Shiitake ( Lentinula edodes )

Mycobiology, Journal Year: 2024, Volume and Issue: 52(6), P. 439 - 445

Published: Nov. 1, 2024

The cultivation period of shiitake (Lentinula edodes) is approximately 120-150 d, which longer than that other edible mushrooms. development fruiting bodies in affected by light exposure. In this study, we investigated the effects blue on production and ergothioneine contents Fruiting body (yield) medium irradiated with increased both cultivars (L808, 555 ± 60 g/3 kg medium; Sanbackhyang, 1090 106 medium). Additionally, antioxidative activities, content L808 (about 2.8 mg/g) was higher Sanbackhyang 1.2 mg/g). These results suggest effective shortening increasing during cultivation.

Language: Английский

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