Complex In Vitro Model Characterization for Context of Use in Toxicologic Pathology: Use Cases by Collaborative Teams of Biologists, Bioengineers, and Pathologists

Toxicologic Pathology, Journal Year: 2024, Volume and Issue: 52(2-3), P. 123 - 137

Published: Feb. 1, 2024

Complex in vitro models (CIVMs) offer the potential to increase clinical relevance of preclinical efficacy and toxicity assessments reduce reliance on animals drug development. The European Society Toxicologic Pathology (ESTP) for (STP) are collaborating highlight role pathologists development use CIVM. Pathologists trained comparative animal medicine which enhances their understanding mechanisms human diseases, thus allowing them bridge between humans. This skill set is important CIVM development, validation, data interpretation. Ideally, diverse teams scientists, including engineers, biologists, pathologists, others, should collaboratively develop characterize novel CIVM, collectively assess precise cases (context use). Implementing a morphological evaluation be essential this process. requires robust histological technique workflows, image analysis techniques, needs correlation with translational biomarkers. In review, we demonstrate how such tissue technologies analytics support safety evaluations. We encourage scientific community explore similar options projects engage health authorities benefit-risk assessment.

Language: Английский

---

Modeling Spinal Cord Injury in a Dish with Hyperosmotic Stress: Population-Specific Effects and the Modulatory Role of Mesenchymal Stromal Cell Secretome

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3298 - 3298

Published: April 2, 2025

Innovations in spinal cord injury (SCI) models are crucial for developing effective therapies. This study introduces a novel vitro SCI model using cultures of primary mixed cells from rat pups, featuring key cell types. offers distinct advantages terms feasibility, reproducibility, and cost-effectiveness, requiring only basic culture equipment. Following hyperosmotic stress via sorbitol treatment, the recapitulated pathophysiological hallmarks, with 65% reduction viability gradual death over 48 h, making it ideal evaluating neuroprotective agents. Notably, human adipose tissue stem (hASC) secretome provided significant protection: preserved metabolic viability, reduced β amyloid precursor protein (β-APP) expression surviving neurons, modulated shift astrocytic morphotype. A transcriptomic profile effect hASC treatment showed functional enrichments related to proliferation cycle progression pathways. In addition supporting use as therapy SCI, this is first hyperosmolar stressor recapitulate aspects pathophysiology. Thereby, can be used promising platform therapeutic agents targeting neuroprotection neuroregeneration, offering outputs death, neuronal stress, protection, well induction glial reactivity.

Language: Английский

---

Seeing beyond Labels: Optical Technologies Reshaping Cell Culture Monitoring in Biomedicine

ACS Applied Engineering Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 4, 2024

Language: Английский

---