Preparative Biochemistry & Biotechnology,
Journal Year:
2025,
Volume and Issue:
unknown, P. 1 - 10
Published: June 2, 2025
This
paper
discusses
the
green
synthesis
of
a
ZnO-NiO
nanocomposite
using
an
extract
citrus
sinensis
peel
as
capping
and
reducing
agent.
The
synthesized
was
characterized
by
X-ray
Diffraction
(XRD),
Energy
Dispersive
Spectroscopy
(EDX),
Scanning
Electron
Microscopy
(SEM),
Fourier
Transform
Infrared
(FTIR),
Ultraviolet-Visible
(UV-Vis)
techniques.
XRD
is
explored
for
crystallinity,
crystalline
size
found
to
be
36
nm
nanocomposite.
EDX
utilized
purity,
FTIR
used
establish
composition
nanocomposites.
SEM
revealed
that
nanocomposites
have
spherical-like
surface
morphology.
bandgap
3.08
eV
from
reflectance
data.
By
calculating
Zone
inhibition
(ZOI)
agar
well
diffusion
method,
antibacterial
activity
(ABA)
investigated
against
three
Gram-positive
strains
five
Gram-negative
bacterial
strains.
ZOI
values
different
ranges
3
±
0.5
mm
16
75
µL
concentration.
findings
in
present
work
demonstrate
with
increase
concentration
nanocomposite,
their
also
increases,
suggesting
its
merit
showing
broad
applicability
various
uses
medicine.
World Journal of Microbiology and Biotechnology,
Journal Year:
2025,
Volume and Issue:
41(5)
Published: May 1, 2025
Abstract
Fungi
serve
as
efficient
biocatalysts
for
the
eco-friendly
synthesis
of
metal
nanoparticles,
yielding
stable
and
bioactive
nanomaterials.
In
this
study,
silver
nanoparticles
were
synthesized
(AgNPs)
using
marine-derived
fungus
Fusarium
equiseti
characterized
them
with
various
analytical
methods.
UV–Vis
spectroscopy
detected
a
surface
plasmon
resonance
peak
at
420
nm,
confirming
AgNP
formation,
while
X-ray
diffraction
(XRD)
verified
their
crystalline
structure.
Scanning
electron
microscopy
(SEM)
transmission
(TEM)
revealed
spherical
averaging
50
nm.
FTIR
analysis
confirmed
that
fungal
metabolites
cap
stabilize
AgNPs.
We
optimized
extracellular
biosynthesis
30°C,
pH
8,
2
mM
AgNO₃
over
72
h.
The
marine-adapted
F.
was
selected
its
robust
metabolic
capacity
enzyme
secretion,
enhancing
nanoparticle
stability
bioactivity.
Biological
assessments
showed
these
AgNPs
outperformed
in
antimicrobial
activity,
minimum
inhibitory
concentrations
(MICs)
6.5
µg/mL
against
Staphylococcus
aureus
7.5
Escherichia
coli
,
plus
antifungal
effects
on
Candida
albicans
solani
.
They
also
displayed
strong
antioxidant
activity
(IC₅₀
=
56.98
µg/mL)
cytotoxicity
MCF-7
breast
cancer
cells
24.38
µg/mL).
These
enhanced
likely
stem
from
acting
natural
capping
agents,
minimizing
aggregation
boosting
bioavailability
biological
interactions.
Molecular
docking
studies
reinforced
results,
revealing
binding
to
microbial
cell
wall
proteins,
human
apoptotic
regulator
Bcl-2
(an
anticancer
target),
peroxiredoxin-5
(PRDX5).
This
green
method
provides
sustainable,
non-toxic
alternative
conventional
chemical
approaches,
avoiding
hazardous
reagents
delivering
stable,
multifunctional
Future
vivo
validation
biocompatibility
are
planned
explore
clinical
pharmaceutical
potential
-derived
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